The Small Auxin-Up RNA 50 (SAUR50) Gene from Ammopiptanthus nanus Negatively Regulates Drought Tolerance
Abstract
:1. Introduction
2. Results
2.1. Cloning and Analysis of AnSAUR50
2.2. Subcellular Localization of AnSAUR50
2.3. Identification of Transgenic Arabidopsis Lines
2.4. Expression of AnSAUR50 Inhibited Drought Tolerance of Transgenic Arabidopssis
2.5. Expression of AnSAUR50 Inhibited Root Length of Transgenic Lines under Drought Stress
2.6. Expression of AnSAUR50 Facilitated Stomatal Aperture of Transgenic Arabidopsis under Drought Stress
3. Discussion
4. Materials and Methods
4.1. Cloning and Bioinformatics Analysis of AnSAUR50
4.2. Identification of Subcellular Localization of AnSAUR50
4.3. Transformation and Screening of Arabidopsis thaliana
4.4. Identification of Target Genes by PCR and RT-PCR
4.5. Phenotyping
4.6. Measurement of Root Length
4.7. Measurement of Stomatal Aperture and Water Loss
4.8. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
- Sato, H.; Mizoi, J.; Shinozaki, K.; Yamaguchi-Shinozaki, K. Complex plant responses to drought and heat stress under climate change. Plant J. 2024, 117, 1873–1892. [Google Scholar] [CrossRef] [PubMed]
- Dietz, K.J.; Zorb, C.; Geilfus, C.M. Drought and crop yield. Plant Biol. 2021, 23, 881–893. [Google Scholar] [CrossRef] [PubMed]
- Gupta, A.; Rico-Medina, A.; Cano-Delgado, A. The physiology of plant responses to drought. Science 2020, 368, 266–269. [Google Scholar] [CrossRef] [PubMed]
- Gonzalez, E.M. Drought stress tolerance in plants. Int. J. Mol. Sci. 2023, 24, 6562. [Google Scholar] [CrossRef]
- Ozturk, M.; Unal, B.T.; Garcia-Caparros, P.; Khursheed, A.; Gul, A.; Hasanuzzaman, M. Osmoregulation and its actions during the drought stress in plants. Physiol. Plant 2021, 172, 1321–1335. [Google Scholar] [CrossRef]
- Razi, K.; Muneer, S. Drought stress-induced physiological mechanisms, signaling pathways and molecular response of chloroplasts in common vegetable crops. Crit. Rev. Biotechnol. 2021, 41, 669–691. [Google Scholar] [CrossRef]
- Gomes, G.L.B.; Scortecci, K.C. Auxin and its role in plant development: Structure, signalling, regulation and response mechanisms. Plant Biol. 2021, 23, 894–904. [Google Scholar] [CrossRef]
- Mishra, B.S.; Sharma, M.; Laxmi, A. Role of sugar and auxin crosstalk in plant growth and development. Physiol. Plant 2022, 174, e13546. [Google Scholar] [CrossRef]
- Jing, H.; Wilkinson, E.G.; Sageman-Furnas, K.; Strader, L.C. Auxin and abiotic stress responses. J. Exp. Bot. 2023, 74, 7000–7014. [Google Scholar] [CrossRef]
- Bao, D.; Chang, S.; Li, X.; Qi, Y. Advances in the study of auxin early response genes: Aux/IAA, GH3, and SAU. Crop J. 2024, 12, 964–978. [Google Scholar] [CrossRef]
- Li, Y.; Han, S.; Qi, Y. Advances in structure and function of auxin response factor in plants. J. Integr. Plant Biol. 2023, 65, 617–632. [Google Scholar] [CrossRef]
- Ren, H.; Gray, W.M. SAUR proteins as effectors of hormonal and environmental signals in plant growth. Mol. Plant 2015, 8, 1153–1164. [Google Scholar] [CrossRef] [PubMed]
- Wu, J.; Liu, S.; He, Y.; Guan, X.; Zhu, X.; Cheng, L.; Wang, J.; Lu, G. Genome-wide analysis of SAUR gene family in Solanaceae species. Gene 2012, 509, 38–50. [Google Scholar] [CrossRef]
- Xu, K.; Lou, Q.; Wang, D.; Li, T.; Chen, S.; Li, T.; Luo, L.; Chen, L. Overexpression of a novel small auxin-up RNA gene, OsSAUR11, enhances rice deep rootedness. BMC Plant Biol. 2023, 23, 319. [Google Scholar] [CrossRef] [PubMed]
- Zhao, Z.; Chen, T.; Yue, J.; Pu, N.; Liu, J.; Luo, L.; Huang, M.; Guo, T.; Xiao, W. Small Auxin Up RNA 56 (SAUR56) regulates heading date in rice. Mol. Breed. 2023, 43, 62. [Google Scholar] [CrossRef]
- Pereyra, M.E.; Rojas, C.C.; Jarrell, A.F.; Hovland, A.S.; Snipes, S.A.; Nagpal, P.; Alabadí, D.; Blázquez, M.A.; Gutiérrez, R.A.; Reed, J.W.; et al. PIF4 enhances the expression of SAUR genes to promote growth in response to nitrate. Proc. Natl. Acad. Sci. USA 2023, 120, e2304513120. [Google Scholar] [CrossRef] [PubMed]
- Huang, X.; Lu, Z.; Zhai, L.; Li, N.; Yan, H. The Small Auxin-Up RNA SAUR10 is involved in the promotion of seedling growth in Rice. Plants 2023, 12, 3880. [Google Scholar] [CrossRef]
- Tian, Z.; Wu, B.; Liu, J.; Zhang, L.; Wu, T.; Wang, Y.; Han, Z.; Zhang, X. Genetic variations in MdSAUR36 participate in the negative regulation of mesocarp cell division and fruit size in Malus species. Mol. Breed. 2024, 44, 1. [Google Scholar] [CrossRef]
- Chen, F.; Wang, R.; Wu, C.; Lin, M.; Yan, H.; Xiang, Y. SAUR8, a small auxin-up RNA gene in poplar, confers drought tolerance to transgenic Arabidopsis plants. Gene 2022, 837, 146692. [Google Scholar] [CrossRef]
- Qiu, T.; Qi, M.Y.; Ding, X.; Zheng, Y.; Zhou, T.; Chen, Y.; Han, N.; Zhu, M.; Bian, H.; Wang, J. The SAUR41 subfamily of SMALL AUXIN UP RNA genes is abscisic acid inducible to modulate cell expansion and salt tolerance in Arabidopsis thaliana seedlings. Ann. Bot. 2020, 125, 805–819. [Google Scholar] [CrossRef]
- Spartz, A.K.; Lor, V.S.; Ren, H.; Olszewski, N.E.; Miller, N.D.; Wu, G.; Spalding, E.P.; Gray, W.M. Constitutive expression of Arabidopsis Small Auxin up RNA19 (SAUR19) in tomato confers Auxin-independent hypocotyl elongation. Plant Physiol. 2017, 173, 1453–1462. [Google Scholar] [CrossRef] [PubMed]
- Spartz, A.K.; Ren, H.; Park, M.Y.; Grandt, K.N.; Lee, S.H.; Murphy, A.S.; Sussman, M.R.; Overvoorde, P.J.; Gray, W.M. SAUR inhibition of PP2C-D phosphatases activates plasma membrane H+-ATPases to promote cell expansion in Arabidopsis. Plant Cell 2014, 26, 2129–2142. [Google Scholar] [CrossRef]
- Liu, Y.; Xiao, L.; Chi, J.; Li, R.; Han, Y.; Cui, F.; Peng, Z.; Wan, S.; Li, G. Genome-wide identification and expression of SAUR gene family in peanut (Arachis hypogaea L.) and functional identification of AhSAUR3 in drought tolerance. BMC Plant Biol. 2022, 22, 178. [Google Scholar] [CrossRef]
- Chen, S.H. Ammopiptanthus Cheng f. A new genus of leguminosae from central Asia. J. Bot. USSR 1959, 44, 1381–1386. [Google Scholar]
- Clough, S.J.; Bent, A.F. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998, 16, 735–743. [Google Scholar] [CrossRef] [PubMed]
- McClure, B.A.; Guilfoyle, T. Characterization of a class of small auxin-inducible soybean polyadenylated RNAs. Plant Mol. Biol. 1987, 9, 611–623. [Google Scholar] [CrossRef] [PubMed]
- Ma, X.; Dai, S.; Qin, N.; Zhu, C.; Qin, J.; Li, J. Genome-wide identification and expression analysis of the SAUR gene family in foxtail millet (Setaria italica L.). BMC Plant Biol. 2023, 23, 31. [Google Scholar] [CrossRef] [PubMed]
- Chen, Y.; Ma, X.; Xue, X.; Liu, M.; Zhang, X.; Xiao, X.; Lai, C.; Zhang, Z.; Lai, Z.; Lin, Y. Genome-wide analysis of the SAUR gene family and function exploration of DlSAUR32 during early longan somatic embryogenesis. Plant Physiol. Biochem. 2023, 195, 362–374. [Google Scholar] [CrossRef]
- Zhang, Y.; Ye, T.; She, Z.; Huang, S.; Wang, L.; Aslam, M.; Qin, R.; Wang, X.; Qin, Y.; Niu, X. Small Auxin Up RNA (SAUR) gene family identification and functional genes exploration during the floral organ and fruit developmental stages in pineapple (Ananas comosus L.) and its response to salinity and drought stresses. Int. J. Biol. Macromol. 2023, 237, 124061. [Google Scholar] [CrossRef]
- Zhu, Q.; Zheng, H.; Hu, X.; Liu, Y.; Zheng, X.; Li, L.; Tang, M. Genome-Wide analysis of the SAUR gene family and its expression profiles in response to salt stress in Santalum album. Plants 2024, 13, 1286. [Google Scholar] [CrossRef]
- Chen, Y.; Hao, X.; Cao, J. Small auxin upregulated RNA (SAUR) gene family in maize: Identification, evolution, and its phylogenetic comparison with Arabidopsis, rice, and sorghum. J. Integr. Plant Biol. 2014, 56, 133–150. [Google Scholar] [CrossRef] [PubMed]
- Park, J.E.; Kim, Y.S.; Yoon, H.K.; Park, C.M. Functional characterization of a small auxin-up RNA gene in apical hook development in Arabidopsis. Plant Ence 2007, 172, 150–157. [Google Scholar] [CrossRef]
- Guo, Y.; Jiang, Q.; Hu, Z.; Sun, X.; Fan, S.; Zhang, H. Function of the auxin-responsive gene TaSAUR75 under salt and drought stress. Crop J. 2018, 6, 181–190. [Google Scholar] [CrossRef]
- He, Y.; Liu, Y.; Li, M.; Lamin-Samu, A.T.; Yang, D.; Yu, X.; Izhar, M.; Jan, I.; Ali, M.; Lu, G. The Arabidopsis SMALL AUXIN UP RNA32protein regulates ABA-mediated responses to drought stress. Front. Plant Sci. 2021, 12, 625493. [Google Scholar] [CrossRef] [PubMed]
- Chae, K.; Isaacs, C.G.; Reeves, P.H.; Maloney, G.S.; Muday, G.K.; Nagpal, P.; Reed, J.W. Arabidopsis SMALL AUXIN UP RNA63 promotes hypocotyl and stamen filament elongation. Plant J. 2012, 71, 684–697. [Google Scholar] [CrossRef]
- Kong, Y.; Zhu, Y.; Gao, C.; She, W.; Lin, W.; Chen, Y.; Han, N.; Bian, H.; Zhu, M.; Wang, J. Tissue-specific expression of small auxin up RNA41 differentially regulates cell expansion and root meristem patterning in Arabidopsis. Plant Cell Physiol. 2013, 54, 609–621. [Google Scholar] [CrossRef]
- Amtmann, A. Learning from evolution: Thellungiella generates new knowledge on essential and critical components of abiotic stress tolerance in plants. Mol. Plant 2009, 2, 3–12. [Google Scholar] [CrossRef]
- Karlova, R.; Boer, D.; Hayes, S.; Testerink, C. Root plasticity under abiotic stress. Plant Physiol. 2021, 187, 1057–1070. [Google Scholar] [CrossRef]
- Spartz, A.K.; Lee, S.H.; Wenger, J.P.; Gonzalez, N.; Itoh, H.; Inzé, D.; Peer, W.A.; Murphy, A.S.; Overvoorde, P.J.; Gray, W.M. The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion. Plant J. 2012, 70, 978–990. [Google Scholar] [CrossRef]
- Ding, L.; Guo, X.; Wang, K.; Pang, H.; Liu, Y.; Yang, Q.; Fu, F.; Li, W.; Yu, H. Genome-wide analysis of BES1/BZR1 transcription factors and their responses to osmotic stress in Ammopiptanthus nanus. J. For. Res. 2021, 26, 127–135. [Google Scholar] [CrossRef]
- Lu, F.Z.; Li, W.C.; Peng, Y.L.; Cao, Y.; Qu, J.T.; Sun, F.A.; Yang, Q.Q.; Lu, Y.L.; Zhang, X.H.; Zheng, L.J.; et al. ZmPP2C26 alternative splicing variants negatively regulate drought tolerance in maize. Front. Plant Sci. 2022, 13, 851531. [Google Scholar] [CrossRef] [PubMed]
- Yu, H.; Liu, B.; Yang, Q.; Yang, Q.; Li, W.; Fu, F. Maize ZmLAZ1-3 gene negatively regulates drought tolerance in transgenic Arabidopsis. BMC Plant Biol. 2024, 24, 246. [Google Scholar] [CrossRef] [PubMed]
- Lv, W.T.; Lin, B.; Zhang, M.; Hua, X.J. Proline accumulation is inhibitory to Arabidopsis seedlings during heat stress. Plant Physiol. 2011, 156, 1921–1933. [Google Scholar] [CrossRef]
- Liu, M.; Chen, J.; Guo, Z.; Lu, S. Differential responses of polyamines and antioxidants to drought in a centipedegrass mutant in comparison to its wild type plants. Front. Plant Sci. 2017, 8, 792. [Google Scholar] [CrossRef] [PubMed]
- Zhang, K.; Xia, X.; Zhang, Y.; Gan, S. An ABA−regulated and Golgi−localized protein phosphatase controls water loss during leaf senescence in Arabidopsis. Plant J. 2012, 69, 667–678. [Google Scholar] [CrossRef]
- Yoo, C.Y.; Pence, H.E.; Jin, J.B.; Miura, K.; Gosney, M.J.; Hasegawa, P.M.; Mickelbart, M.V. The Arabidopsis GTL1 transcription factor regulates water use efficiency and drought tolerance by modulating stomatal density via transrepression of SDD1. Plant Cell 2010, 22, 4128–4141. [Google Scholar] [CrossRef]
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Zhang, Y.; Li, Q.; Jiang, M.; Tian, H.; Khalid, M.H.B.; Wang, Y.; Yu, H. The Small Auxin-Up RNA 50 (SAUR50) Gene from Ammopiptanthus nanus Negatively Regulates Drought Tolerance. Plants 2024, 13, 2512. https://doi.org/10.3390/plants13172512
Zhang Y, Li Q, Jiang M, Tian H, Khalid MHB, Wang Y, Yu H. The Small Auxin-Up RNA 50 (SAUR50) Gene from Ammopiptanthus nanus Negatively Regulates Drought Tolerance. Plants. 2024; 13(17):2512. https://doi.org/10.3390/plants13172512
Chicago/Turabian StyleZhang, Yuanyuan, Qi Li, Mengyang Jiang, Hui Tian, Muhammad Hayder Bin Khalid, Yingge Wang, and Haoqiang Yu. 2024. "The Small Auxin-Up RNA 50 (SAUR50) Gene from Ammopiptanthus nanus Negatively Regulates Drought Tolerance" Plants 13, no. 17: 2512. https://doi.org/10.3390/plants13172512