Assisted Phytoremediation between Biochar and Crotalaria pumila to Phytostabilize Heavy Metals in Mine Tailings
Abstract
:1. Introduction
2. Results
2.1. Physicochemical Characterization and Heavy Metal Analysis of the Coconut Fiber Biochar
2.2. Bioaccumulation of Heavy Metals in Root and Leaf Tissue in C. pumila Individuals That Grow in Mine Tailing Substrate and Mine Tailing Substrate/Biochar
2.3. Metal Bioconcentration and Translocation Factors in Roots and Leaves in C. pumila Individuals Growing on Mine Tailing and Mine Tailing/Biochar Substrates
2.4. Effect of the Substrate (Mine Tailing and Mine Tailing/Biochar) on C. pumila Biomass
2.5. Effect of the Substrate (Mine Tailing and Mine Tailing/Biochar) on Chlorophyll a and b Content in C. pumila Individuals
2.6. Effect of the Substrate (Mine Tailing and Mine Tailing/Biochar) on Genetic Damage in C. pumila Individuals
3. Discussion
3.1. Influence of Biochar on Mine Tailing Substrate in the Bioaccumulation of Heavy Metals in C. pumila Individuals
3.2. Influence of Biochar on Bioconcentration and Translocation of Heavy Metals in C. pumila Individuals
3.3. Influence of Biochar on Mine Tailing Substrate on the Biomass of C. pumila
3.4. Influence of Biochar on the Mine Tailing Substrate on Chlorophyll a and b Contents and Genetic Damage in C. pumila
4. Materials and Methods
4.1. Study Sites
4.2. Study Species
4.3. Seed Collection, Germination, and Obtaining Seedlings
4.4. Biochar Production and Physicochemical Characterization
4.5. Determination of Plant Biomass
4.6. Analysis of Heavy Metal Concentration
4.7. Determination of Bioconcentration Factor (BCF) and Translocation Factor (TF)
4.8. Genetic Damage: Alkaline Gel Electrophoresis
4.9. Determination of Chlorophyll Content
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Concentration (mg kg−1) | |||||||
---|---|---|---|---|---|---|---|
Metals | Time (Days) | BCF (Root) | BCF (Leaf) | TF | TF | ||
Mine Tailing | Mine Tailing/Biochar | Mine Tailing | Mine Tailing/Biochar | Mine Tailing | Mine Tailing/Biochar | ||
Zinc | |||||||
25 | 1.15 | 0.37 | 0.18 | 0.14 | 0.16 | 0.37 | |
50 | 1.47 | 0.95 | 0.28 | 0.23 | 0.19 | 0.24 | |
75 | 1.63 | 0.85 | 0.54 | 0.21 | 0.33 | 0.25 | |
100 | 1.90 | 0.76 | 0.57 | 0.21 | 0.30 | 0.28 | |
Average ± SD | 1.54 ± 0.32 | 0.73 ± 0.26 | 0.39 ± 0.19 | 0.20 ± 0.04 | 0.25 ± 0.08 | 0.29 ± 0.06 | |
t-student | 24.049 *** | 12.213 *** | 3.917 *** | ||||
Cooper | |||||||
25 | 18.70 | 14.00 | 12.71 | 10.50 | 0.68 | 0.75 | |
50 | 26.67 | 18.00 | 14.71 | 11.88 | 0.55 | 0.66 | |
75 | 28.29 | 11.25 | 15.46 | 6.63 | 0.55 | 0.59 | |
100 | 26.30 | 9.25 | 13.84 | 5.13 | 0.53 | 0.55 | |
Average ± SD | 24.99 ± 4.28 | 13.13 ± 3.79 | 14.18 ± 1.18 | 8.53 ± 3.18 | 0.58 ± 0.07 | 0.63 ± 0.09 | |
t-student | 28.174 *** | 22.237 *** | 5.537 *** | ||||
Lead | |||||||
25 | 124.35 | 51.57 | 28.26 | 8.86 | 0.23 | 0.17 | |
50 | 157.20 | 59.71 | 58.09 | 11.71 | 0.37 | 0.20 | |
75 | 167.53 | 67.43 | 56.51 | 12.57 | 0.34 | 0.19 | |
100 | 140.28 | 44.00 | 55.22 | 8.14 | 0.39 | 0.19 | |
Average ± SD | 147.34 ± 19 | 55.68 ± 10.13 | 49.52 ± 14.22 | 10.32 ± 2.15 | 0.33 ± 0.07 | 0.18 ± 0.01 | |
t-student | 55.367 *** | 34.707 *** | 22.768 *** | ||||
Cadmium | |||||||
25 | 12.79 | 3.93 | 6.69 | 1.67 | 0.52 | 0.42 | |
50 | 15.90 | 3.57 | 6.10 | 1.31 | 0.38 | 0.37 | |
75 | 15.30 | 2.62 | 5.74 | 1.07 | 0.38 | 0.41 | |
100 | 14.58 | 1.79 | 3.23 | 0.00 | 0.22 | 0.00 | |
Average ± SD | 14.64 ± 1.35 | 2.98 ± 0.97 | 5.44 ± 1.53 | 1.01 ± 0.12 | 0.38 ± 0.12 | 0.30 ± 0.20 | |
t-student | 89.419 *** | 33.574 *** | 3.462 *** |
Predicted | |||||||||
---|---|---|---|---|---|---|---|---|---|
Type of Measurement | Metal | Sample []soil | Sample []plant | Adjusted BCF | Excluder | Indicator | Accumulator | Hyperaccumulator | Result |
Root | Pb | 6.972 | 910.27 | 370.39 | Hyperaccumulator | ||||
Cd | 8.365 | 12.62 | 42.18 | 0.810 | 3.000 | 16.155 | 61.547 | Accumulator | |
Cu | 8.023 | 211.75 | 74.76 | Hyperaccumulator | |||||
Zn | 428.114 | 815.54 | 39.42 | 1.675 | 6.201 | 33.395 | 127.230 | Accumulator | |
Leaves | Pb | 6.972 | 132.3 | 145.81 | Hyperaccumulator | ||||
Cd | 8.365 | 12.53 | 9.34 | 0.554 | 2.052 | 11.052 | 42.106 | Accumulator | |
Cu | 8.023 | 111.05 | 39.21 | Accumulator | |||||
Zn | 428.114 | 243.23 | 11.76 | 1.146 | 4.242 | 22.847 | 87.043 | Accumulator |
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Rosas-Ramírez, M.; Tovar-Sánchez, E.; Rodríguez-Solís, A.; Flores-Trujillo, K.; Castrejón-Godínez, M.L.; Mussali-Galante, P. Assisted Phytoremediation between Biochar and Crotalaria pumila to Phytostabilize Heavy Metals in Mine Tailings. Plants 2024, 13, 2516. https://doi.org/10.3390/plants13172516
Rosas-Ramírez M, Tovar-Sánchez E, Rodríguez-Solís A, Flores-Trujillo K, Castrejón-Godínez ML, Mussali-Galante P. Assisted Phytoremediation between Biochar and Crotalaria pumila to Phytostabilize Heavy Metals in Mine Tailings. Plants. 2024; 13(17):2516. https://doi.org/10.3390/plants13172516
Chicago/Turabian StyleRosas-Ramírez, Marcos, Efraín Tovar-Sánchez, Alexis Rodríguez-Solís, Karen Flores-Trujillo, María Luisa Castrejón-Godínez, and Patricia Mussali-Galante. 2024. "Assisted Phytoremediation between Biochar and Crotalaria pumila to Phytostabilize Heavy Metals in Mine Tailings" Plants 13, no. 17: 2516. https://doi.org/10.3390/plants13172516