Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Sucrose Content of 1014 Soybean Accessions
2.2. Sucrose Content Analysis of Two F2:3 Populations
2.3. Genotyping and Linkage Map Construction
2.4. Quantitative Trait Locus (QTL) Analysis
2.5. Identification of Candidate Genes for Sucrose Content
2.6. mRNA Expression Analysis of Sucrose-Related Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. GOD/INV Analysis of the Sucrose Content
4.3. Population Development
4.4. DNA Extraction and SNP Genotyping
4.5. Genetic Mapping and QTL Analysis
4.6. RT-qPCR Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Genotype | Origin | Sucrose Content (%) | ||
---|---|---|---|---|
2020 | 2021 | |||
High sucrose | IT186230 | KOR | 6.44 ± 0.09 | 7.77 ± 0.03 |
IT195321 | COL | 6.34 ± 0.06 | 6.60 ± 0.07 | |
IT263138 | KOR | 7.37 ± 0.18 | 7.61 ± 0.01 | |
IT263276 | KOR | 6.97 ± 0.04 | 7.43 ± 0.09 | |
IT263286 | KOR | 7.27 ± 0.25 | 7.65 ± 0.00 | |
IT276521 | KOR | 6.60 ± 0.37 | 7.49 ± 0.06 | |
Low sucrose | IT025668 | KOR | 1.23 ± 0.00 | 1.71 ± 0.03 |
IT274054 | CSK † | 1.85 ± 0.11 | 2.18 ± 0.05 | |
Control | Ilmi | KOR | - | 7.39 ± 0.03 |
Populations | Parent | F2:3 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
P1 † | P2 | N | Min. | Max. | Mean. | CV | Skew. | Kurt. | h2 | |
IT186230 × IT025668 | 7.25 | 1.73 | 147 | 2.66 | 6.22 | 4.27 | 15.33 | 0.51 | 0.32 | 0.96 |
Ilmi × IT186230 | 4.73 | 7.25 | 188 | 3.79 | 7.59 | 6.21 | 12.39 | −0.60 | −0.14 | 0.95 |
QTL | Chr. | Interval | Physical Interval (Mb) | LOD | Peak or Flanking Marker | IT186230 Allele Effect (%) | IT025668 Allele Effect (%) | R2 | Additive Effect |
---|---|---|---|---|---|---|---|---|---|
qSUC6.1 | 6 | Gm06_790146–Gm06_2393421 | 0.7–2.3 | 5.12 | Gm06_1655912 | 4.3 | 3.5 | 13.4 | 0.39 |
QTL | Chr. | Interval | Physical Interval (Mb) | LOD | Peak or Flanking Marker | Ilmi Allele Effect (%) | IT186230 Allele Effect (%) | R2 | Additive Effect |
---|---|---|---|---|---|---|---|---|---|
qSUC11.1 | 11 | Gm11_17237725–Gm11_24186948 | 17.2–24.1 | 5.31 | Gm11_17808678 | 5.9 | 6.6 | 9.6 | –0.32 |
qSUC15.1 | 15 | Gm15_29706012–Gm15_40878937 | 29.7–40.8 | 13.6 | Gm15_33001719 | 6.8 | 5.7 | 27.6 | 0.56 |
qSUC17.1 | 17 | Gm17_12210561–Gm17_13127250 | 12.2–13.1 | 4.1 | Gm17_12842825 | 6.1 | 6.4 | 7.3 | –0.18 |
QTL | Gene ID | Physical Position (bp) | Annotation | Protein ID |
---|---|---|---|---|
qSUC6.1 | ||||
Glyma.06G011700 | Gm06:873613..878088 | Glucose-1-phosphate adenylyl transferase family protein | ||
Glyma.06G015000 | Gm06:1127434..1132895 | Tonoplast monosaccharide transporter 2 | ||
Glyma.06G015900 | Gm06:1187405..1190649 | Glyceraldehyde-3-phosphate dehydrogenase B subunit | ||
Glyma.06G018000 | Gm06:1359828..1368182 | 1,4-alpha-glucan-branching enzyme/starch-branching enzyme II | ||
Glyma.06G028800 | Gm06:2237126..2239413 | Fructose-2,6-bisphosphatase | ||
Glyma.06G030400 | Gm06:2390673..2396324 | Glucose-1-phosphate adenylyltransferase large subunit 2 | ||
Glyma.06G032500 | Gm06:2515199..2519958 | Glucose-6-phosphate isomerase | ||
qSUC11.1 | ||||
Glyma.11G148900 | Gm11:11489777..11495616 | Glycerol-3-phosphate dehydrogenase | ||
Glyma.11G150600 | Gm11:11808846..11810660 | Sugar-1-phosphate guanyl transferase | ||
qSUC15.1 | ||||
Glyma.15G210100 | Gm15:32175249..32210061 | Trehalose-6-phosphate synthase | ||
Glyma.15g210200 | Gm15:32217111..32218567 | Protein kinase superfamily protein | ||
Glyma.15G210400 | Gm15:32262290..32264065 | RAG1-activating protein 1 | SWEET41 | |
Glyma.15G211800 | Gm15:32835023..32838031 | RAG1-activating protein 1 | SWEET42 | |
Glyma.15G212600 | Gm15:33402757..33405141 | Glutathione S-transferase THETA 2 | ||
Glyma.15G221300 | Gm15:39951868..39954192 | UDP-glucosyl transferase 73B3 | ||
qSUC17.1 | ||||
Glyma.17G137200 | Gm17:11073919..11077576 | Nucleotide-sugar transporter family protein | ||
Glyma.17G137500 | Gm17:11097469..11100931 | UDP-glycosyltransferase superfamily protein | ||
Glyma.17G138700 | Gm17:11248118..11252772 | Trehalose-phosphate synthase | ||
Glyma.17G152300 | Gm17:12696012..12697117 | Triose-phosphate transporter family | ||
Glyma.17G152400 | Gm17:12704557..12706988 | UDP-galactose transporter |
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Kang, S.-H.; Shin, S.-Y.; Kang, B.H.; Chowdhury, S.; Lee, W.-H.; Kim, W.J.; Lee, J.-D.; Lee, S.; Choi, Y.-M.; Ha, B.-K. Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean. Plants 2024, 13, 2815. https://doi.org/10.3390/plants13192815
Kang S-H, Shin S-Y, Kang BH, Chowdhury S, Lee W-H, Kim WJ, Lee J-D, Lee S, Choi Y-M, Ha B-K. Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean. Plants. 2024; 13(19):2815. https://doi.org/10.3390/plants13192815
Chicago/Turabian StyleKang, Se-Hee, Seo-Young Shin, Byeong Hee Kang, Sreeparna Chowdhury, Won-Ho Lee, Woon Ji Kim, Jeong-Dong Lee, Sungwoo Lee, Yu-Mi Choi, and Bo-Keun Ha. 2024. "Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean" Plants 13, no. 19: 2815. https://doi.org/10.3390/plants13192815