Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation
Abstract
:1. Introduction
2. Results
2.1. Identification of Differentially Expressed Genes Using RNA Sequencing
2.2. Functional Annotation of Identified DEGs Induced by Salt Stress
2.3. The Expression Patterns of DEGs Identified in the Salt-Tolerant Mutant
2.4. Validation of Selected DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Salt Treatment
4.2. RNA Isolation, Short-Read Sequencing, and Mapping of Clean Reads
4.3. Identification and Functional Annotation Analysis of Differentially Expressed Genes (DEGs)
4.4. Reverse Transcription followed by Quantitative Polymerase Chain Reaction (RT-qPCR)
4.5. Quantification of Acetyl Bromide Soluble Lignin (ABSL)
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Tissues | Time Points | Number of DEGs | Total DEGs | |
---|---|---|---|---|
Upregulated | Downregulated | |||
Leaves | 0 h | 161 | 26 | 187 |
24 h | 30 | 30 | 60 | |
72 h | 33 | 16 | 49 | |
Roots | 0 h | 42 | 25 | 67 |
24 h | 4 | 51 | 55 | |
72 h | 36 | 12 | 48 |
Gene ID | Log2 (Fold Change) | Annotation | EC * Number | |||||
---|---|---|---|---|---|---|---|---|
Leaf | Root | |||||||
0 h | 24 h | 72 h | 0 h | 24 h | 72 h | |||
Glyma.02G234200.1 | 2.51 | −0.07 | 0.02 | 1.20 | 0.51 | −0.05 | Peroxidase superfamily protein | 1.11.1.7 |
Glyma.03G145600.1 | 4.90 | −0.69 | −0.32 | 0.19 | −0.60 | −0.38 | Peroxidase 2 | 1.11.1.7 |
Glyma.04G220600.1 | 3.78 | 0.07 | −1.72 | 0.50 | −0.31 | −0.38 | Peroxidase superfamily protein | 1.11.1.7 |
Glyma.05G231800.1 | 4.38 | −0.11 | 0.35 | −0.25 | −0.06 | 0.83 | Aldehyde dehydrogenase 2C4 | 1.2.1.68 |
Glyma.09G022300.1 | 2.30 | 1.17 | 0.39 | 0.12 | 0.38 | −0.21 | Peroxidase 2 | 1.11.1.7 |
Glyma.14G201800.1 | 2.22 | −0.30 | −0.03 | 0.30 | 0.22 | 0.27 | Peroxidase superfamily protein | 1.11.1.7 |
Glyma.19G091800.1 | 2.36 | 0.25 | −0.28 | 0.50 | 0.12 | −0.28 | Peroxidase superfamily protein | 1.11.1.7 |
Glyma.20G003500.1 | 2.59 | 0.23 | 0.61 | 0.26 | 0.04 | −0.01 | O-methyltransferase 1 | 2.1.1.68 |
Glyma.20G045800.1 | −1.54 | −2.93 | −1.62 | 0.63 | −1.19 | 0.66 | Spermidine hydroxycinnamoyl transferase | 2.3.1.133 |
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Kang, B.H.; Chowdhury, S.; Kang, S.-H.; Shin, S.-Y.; Lee, W.-H.; Lee, H.-S.; Ha, B.-K. Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation. Plants 2024, 13, 254. https://doi.org/10.3390/plants13020254
Kang BH, Chowdhury S, Kang S-H, Shin S-Y, Lee W-H, Lee H-S, Ha B-K. Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation. Plants. 2024; 13(2):254. https://doi.org/10.3390/plants13020254
Chicago/Turabian StyleKang, Byeong Hee, Sreeparna Chowdhury, Se-Hee Kang, Seo-Young Shin, Won-Ho Lee, Hyeon-Seok Lee, and Bo-Keun Ha. 2024. "Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation" Plants 13, no. 2: 254. https://doi.org/10.3390/plants13020254