Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth
Abstract
:1. Introduction
2. Results
2.1. Affinity Purification and Mass Spectrometry Analysis Experimental Design
2.2. Identification of Putative PiCDPK1 Substrates
2.3. Bioinformatic Analysis of Filtered Putative PiCDPK1 Substrates
2.4. Stable Over-Expression of NtGF14 in Tobacco Pollen Tubes
2.5. Stable Over-Expression of NtREN1 in Tobacco Pollen Tubes
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions and Pollen Tube Germination
4.2. Protein Extraction and Affinity Purification
4.3. Sample Preparation for Proteomics Analysis by Liquid Chromatography-Mass Spectrometry
4.4. Proteomics Analysis by Liquid Chromatography-Mass Spectrometry
4.5. Stable Over-Expression of NtGF14 and NtREN1 in Tobacco
4.6. Analysis of Transformed Pollen Tubes
4.7. Computational Analysis
4.8. Quantitative RT-PCR
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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N. tabacum ID | Arabidopsis Homolog | Gene Family | Putative Function | |
---|---|---|---|---|
ID | Gene Name | |||
A0A1S4BR59 | AT5G12180 | CPK 17 | Calcium Dependent Protein Kinase | Transduce Ca2+ signals to modulate pollen tube growth [30] |
Q75ZD6 | AT1G78300 | GF14 Omega | 14-3-3 protein | Facilitates interaction of actin-bundling plasma membrane proteins [31,32] |
A0A1S3ZQG5 | AT4G24580 | ROP1 enhancer 1 | Rho GTPase activation protein (RhoGAP) with PH domain | Inhibits activation of ROP1, regulates pollen tube growth [33] |
A0A024AYA4 | AT3G48700 | Carboxyesterase 13 | Carboxyesterase | Hydrolysis of esters [34] |
A0A077DBK4 | AT5G12020 | 17.6 kDa class II heat shock protein | Heat-shock protein | Interacts with and activates catalase in peroxisomes [35] |
A0A0S0N4U0 | AT5G19770 | Tubulin alpha-3 | Tubulin | Maintenance of growth against gravitational force [36] |
A0A1S3X6C4 | AT1G14420 | Pectate lyase-like 8 | Pectate lyase protein | Pectin synthesis/remodeling and secondary wall formation [37] |
A0A1S3X7U8 | AT2G47040 | Vanguard 1 | Plant invertase/pectin methylesterase inhibitor superfamily | Enhancer of growth of pollen tube in female floral tissues [38] |
A0A1S3XSQ7 | AT5G17820 | Peroxidase 57 | Peroxidase superfamily protein | Affects permeability of leaf cuticle [39] |
A0A1S3XWB5 | AT3G17390 | S-adenosylmethionine synthetase 3 | S-adenosylmethionine synthetase family protein | Involved in salt and H2O2 stress tolerance [40] |
A0A1S3YE49 | AT3G22530 | Unknown | Hypothetical heat-shock protein | Unknown |
A0A1S3Z0D6 | AT1G34340 | Unknown | Hypothetical alpha/beta-Hydrolase superfamily protein | Unknown |
A0A1S3Z6E1 | AT2G32730 | Unknown | Hypothetical 26S proteasome regulatory complex | Unknown |
A0A1S3Z926 | AT3G59290 | Epsin 3 | Epsin family of endocytic proteins | Accessory proteins that facilitate vesicle biogenesis [41] |
A0A1S4AFS9 | AT3G19940 | Sugar Transport Protein 10 | Sugar Transport Protein | Glucose uptake in growing pollen tubes [42] |
A0A1S4AKF1 | AT1G16010 | Magnesium Transporter 2 | Transmembrane magnesium transporter | Tonoplast-targeted transporter for vacuolar accumulation of magnesium [43] |
A0A1S4C178 | AT5G09690 | Magnesium Transporter 7 | Transmembrane magnesium transporter | Low-affinity Mg2+ transporter [44] |
A0A1S4CMX7 | AT3G59990 | Methionine aminopeptidase 2b | Methionine Amino-peptidase (MAP) | Required for development at various stages of Arabidopsis life cycle [45] |
A0A1S4CPM1 | AT4G37760 | Squalene epoxidase 3 | Squalene epoxidase enzyme | Plays a role in sterol biosynthesis in shoots [46] |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Scheible, N.; Henning, P.M.; McCubbin, A.G. Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth. Plants 2024, 13, 451. https://doi.org/10.3390/plants13030451
Scheible N, Henning PM, McCubbin AG. Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth. Plants. 2024; 13(3):451. https://doi.org/10.3390/plants13030451
Chicago/Turabian StyleScheible, Nolan, Paige M. Henning, and Andrew G. McCubbin. 2024. "Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth" Plants 13, no. 3: 451. https://doi.org/10.3390/plants13030451