Susceptibility of Field and Laboratory Bt-Susceptible and Resistant Strains of Helicoverpa zea (Boddie) to HearNPV
Abstract
:1. Introduction
2. Results
2.1. Dose-Response Bioassays in 2021
2.2. Dose-Response Bioassays in 2022
2.3. Correlation Analysis between HearNPV and Bt Resistance
3. Discussion
4. Materials and Methods
4.1. Laboratory Insect Strains
4.2. Field-Collected Insect Strains
4.3. Preliminary Tests for Determining HearNPV Concentrations
4.4. Dose-Response Bioassays
4.5. Data Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Insect Strain | Host | n a | LC50 (95% FL) (OBs/mL) | Slope ± SE | x2 | df | Resistance Ratio (RR) b |
---|---|---|---|---|---|---|---|
2021 | |||||||
SS | Lab diet | 840 | 6.5 × 103 (4.9 × 103, 8.4 × 103) | 1.7 ± 0.2 | 119 | 22 | 1 |
CRY-RR | Lab diet | 840 | 71.0 × 103 (54.6 × 103, 99.8 × 103) | 1.4 ± 0.1 | 104 | 28 | 10.9 * |
VIP-RR-70 | Lab diet | 1050 | 10.5 × 103 (8.0 × 103, 14.0 × 103) | 1.3 ± 0.1 | 128 | 28 | 1.6 |
Thrall-TX | Intrasect corn | 560 | 147.3 × 103 (64.4 × 103, 3625.0 × 103) | 1.3 ± 0.4 | 9.5 | 22 | 22.7 * |
Malone-TX | DoublePro corn | 616 | 62.6 × 103 (43.1 × 103, 100.0 × 103) | 1.2 ± 0.2 | 60.4 | 22 | 9.5 * |
Winnsboro-LA | DoublePro corn | 630 | 66.9 × 103 (33.3 × 103, 241.3 × 103) | 0.8 ± 0.2 | 25.2 | 22 | 10.3 * |
Alexandria-LA | Trecepta corn | 336 | 134.4 × 103 (54.2 × 103, 1623.9 × 103) | 0.9 ± 0.2 | 14.7 | 16 | 20.7 * |
2022 | |||||||
SS | Lab diet | 448 | 2.8 × 103 (2.1 × 103, 3.7 × 103) | 1.9 ± 0.2 | 86.6 | 22 | 1 |
VIP-RR-15 | Lab diet | 448 | 1.8 × 103 (0.8 × 103, 3.1 × 103) | 1.0 ± 0.2 | 39.2 | 22 | 0.7 |
TRE-RR | Lab diet | 238 | 1.6 × 103 (0.9 × 103, 2.4 × 103) | 1.2 ± 0.2 | 47.7 | 16 | 0.6 |
Epps-LA | Crimson clover | 336 | 490.0 × 103 (100.0 × 103, 4.9 × 108) | 0.6 ± 0.2 | 10.2 | 16 | 175.0 * |
Leland-MS | NBT corn | 448 | 5.5 × 103 (4.0 × 103, 7.5 × 103) | 1.7 ± 0.2 | 89.6 | 22 | 2.0 * |
Marianna-AR | NBT corn | 448 | 5.0 × 103 (4.0 × 103, 6.3 × 103) | 1.8 ± 0.1 | 147 | 22 | 1.8 * |
Taylor-TX | NBT corn | 448 | 10.2 × 103 (6.2 × 103, 17.0 × 103) | 0.6 ± 0.1 | 45.4 | 22 | 3.6 * |
Insect Strain | Cry1Ac | Cry2Ab2 | Vip3Aa39 | Reference |
---|---|---|---|---|
CRY-RR | 779.3 * | 387.8 * | 0.4 | [32] |
VIP-RR-70 | 6.3 | 1.9 | >892.8 * | [33] |
VIP-RR-15 | 2.0 | 1.2 | >892.8 * | [33] |
TRE-RR | 264.8 * | 495.3 * | >95.7 * | [33] |
Thrall-TX | 2,787,333 * | 64.8 * | <0.09 | [34] |
Malone-TX | 539.6 * | 67.3 * | <0.09 | [34] |
Taylor-TX | 55,028.6 * | 12.0 * | 0.2 | [35] |
Winnsboro-LA | 154.6 * | 79.4 * | 0.21 | [34] |
Alexandria-LA | / | 5.31 | <0.09 | [34] |
Epps-LA | 351.1 * | 740.7 * | 1.9 | [35] |
Leland-MS | 72,150.0 * | 13.2 * | 0.04 | [35] |
Marianna-AR | 1498.6 * | 8.0 | 0.3 | [35] |
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Calvin, W.; Yang, F.; Kennedy, H.; Marçon, P.G.; Kerns, D.L. Susceptibility of Field and Laboratory Bt-Susceptible and Resistant Strains of Helicoverpa zea (Boddie) to HearNPV. Plants 2024, 13, 529. https://doi.org/10.3390/plants13040529
Calvin W, Yang F, Kennedy H, Marçon PG, Kerns DL. Susceptibility of Field and Laboratory Bt-Susceptible and Resistant Strains of Helicoverpa zea (Boddie) to HearNPV. Plants. 2024; 13(4):529. https://doi.org/10.3390/plants13040529
Chicago/Turabian StyleCalvin, Wilfrid, Fei Yang, Haley Kennedy, Paula G. Marçon, and David L. Kerns. 2024. "Susceptibility of Field and Laboratory Bt-Susceptible and Resistant Strains of Helicoverpa zea (Boddie) to HearNPV" Plants 13, no. 4: 529. https://doi.org/10.3390/plants13040529