The Receptor Kinases DRUS1 and DRUS2 Behave Distinctly in Osmotic Stress Tolerance by Modulating the Root System Architecture via Auxin Signaling
Abstract
:1. Introduction
2. Results
2.1. drus1-1 and drus2 Mutants Displayed Distinct Tolerance to Osmotic Stress
2.2. drus2 but Not drus1-1 Seedlings Displayed a Strong Root System under OS
2.3. DRUS1 but Not DRUS2 Is Highly Induced in the Root by OS at Both the mRNA Level and the Protein Level
2.4. drus2 but Not drus1-1 Seedlings Are Insensitive to ABA Inhibition
2.5. The Root Growth of drus1-1 Is Sensitive But drus2 Is Insensitive to Auxin Deprivation
2.6. The OsIAAs Are Highly Induced in drus1-1 but Not in drus2 Roots
3. Discussion
3.1. DRUS1 Plays a Unique Role in the Osmotic Stress Tolerance of Rice Plants
3.2. DRUS1 Promotes Root Expansion under OS by Repressing OsIAAs and Activating Auxin Signaling
3.3. The Biological Significance of the Presence of DRUS1 and DRUS2 Homologues in Rice Genome
4. Materials and Methods
4.1. Plant Materials and Growth Conditions under Osmotic Stress
4.2. GUS Staining Assay
4.3. Immunoblot Analysis
4.4. Quantitative Reverse Transcription-PCR (qRT-PCR)
4.5. Auxin Content Measurement
4.6. Yucasin Treatment
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Latif, A.; Yang, C.-G.; Zhang, L.-X.; Yang, X.-Y.; Liu, X.-Y.; Ai, L.-F.; Noman, A.; Pu, C.-X.; Sun, Y. The Receptor Kinases DRUS1 and DRUS2 Behave Distinctly in Osmotic Stress Tolerance by Modulating the Root System Architecture via Auxin Signaling. Plants 2024, 13, 860. https://doi.org/10.3390/plants13060860
Latif A, Yang C-G, Zhang L-X, Yang X-Y, Liu X-Y, Ai L-F, Noman A, Pu C-X, Sun Y. The Receptor Kinases DRUS1 and DRUS2 Behave Distinctly in Osmotic Stress Tolerance by Modulating the Root System Architecture via Auxin Signaling. Plants. 2024; 13(6):860. https://doi.org/10.3390/plants13060860
Chicago/Turabian StyleLatif, Ammara, Chen-Guang Yang, Lan-Xin Zhang, Xin-Yu Yang, Xin-Ye Liu, Lian-Feng Ai, Ali Noman, Cui-Xia Pu, and Ying Sun. 2024. "The Receptor Kinases DRUS1 and DRUS2 Behave Distinctly in Osmotic Stress Tolerance by Modulating the Root System Architecture via Auxin Signaling" Plants 13, no. 6: 860. https://doi.org/10.3390/plants13060860