Physiological and Proteomic Analyses of mtn1 Mutant Reveal Key Players in Centipedegrass Tiller Development
Abstract
:1. Introduction
2. Results
2.1. Phenotype of mtn1 Mutant
2.2. Physiological Characteristics
2.3. Protein Data Quality
2.4. Differential Expression Proteins Analysis
2.5. Differentially Regulated Biochemical Pathways of mtn1 Mutant and WT
2.6. DEPs Involved in Plant Hormone Signal Transduction
2.7. DAPs Involved in Starch and Sucrose Metabolism
2.8. qRT-PCR
2.9. Summary Analysis of the Physiological Parameters and Proteomes
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Experimental Design
4.2. Physiological Parameters’ Determination
4.3. Protein Extraction and Trypsin Digestion
4.4. Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Analysis
4.5. Protein Identification and Bioinformatics Analysis
4.6. qRT-PCR
4.7. Data Statistics and Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Xie, C.; Chen, R.; Sun, Q.; Hao, D.; Zong, J.; Guo, H.; Liu, J.; Li, L. Physiological and Proteomic Analyses of mtn1 Mutant Reveal Key Players in Centipedegrass Tiller Development. Plants 2024, 13, 1028. https://doi.org/10.3390/plants13071028
Xie C, Chen R, Sun Q, Hao D, Zong J, Guo H, Liu J, Li L. Physiological and Proteomic Analyses of mtn1 Mutant Reveal Key Players in Centipedegrass Tiller Development. Plants. 2024; 13(7):1028. https://doi.org/10.3390/plants13071028
Chicago/Turabian StyleXie, Chenming, Rongrong Chen, Qixue Sun, Dongli Hao, Junqin Zong, Hailin Guo, Jianxiu Liu, and Ling Li. 2024. "Physiological and Proteomic Analyses of mtn1 Mutant Reveal Key Players in Centipedegrass Tiller Development" Plants 13, no. 7: 1028. https://doi.org/10.3390/plants13071028