Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings

Uenishi, Yumi; Nakabayashi, Yukari; Tsuchihira, Ayako; Takusagawa, Mari; Hashimoto, Kayo; Maeshima, Masayoshi; Sato-Nara, Kumi

doi:10.3390/plants3010177

Open AccessArticle

Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings

by

Yumi Uenishi

^1,†,

Yukari Nakabayashi

^2,‡,

Ayako Tsuchihira

³,

Mari Takusagawa

^1,§,

Kayo Hashimoto

¹,

Masayoshi Maeshima

³ and

Kumi Sato-Nara

^2,*

¹

Graduate School of Humanities and Sciences, Nara Women's University, Nara 630-8506, Japan

²

Faculty of Science, Nara Women's University, Nara 630-8506, Japan

³

Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

^*

Author to whom correspondence should be addressed.

^†

Present address: NIPPON Funmatsu Yakuhin CO., Ltd, Osaka 541-0045, Japan

^‡

Present address: Graduate School of Information Science, Nagoya University, Nagoya 464-8601, Japan

^§

Present address: Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan;

Plants 2014, 3(1), 177-195; https://doi.org/10.3390/plants3010177

Submission received: 15 November 2013 / Revised: 2 February 2014 / Accepted: 21 February 2014 / Published: 5 March 2014

(This article belongs to the Special Issue Plant Light Signalling)

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Abstract

Light regulates the expression and function of aquaporins, which are involved in water and solute transport. In Arabidopsis thaliana, mRNA levels of one of the aquaporin genes, TIP2;2, increase during dark adaptation and decrease under far-red light illumination, but the effects of light at the protein level and on the mechanism of light regulation remain unknown. Numerous studies have described the light regulation of aquaporin genes, but none have identified the regulatory mechanisms behind this regulation via specific photoreceptor signaling. In this paper, we focus on the role of phytochrome A (phyA) signaling in the regulation of the TIP2;2 protein. We generated Arabidopsis transgenic plants expressing a TIP2;2-GFP fusion protein driven by its own promoter, and showed several differences in TIP2;2 behavior between wild type and the phyA mutant. Fluorescence of TIP2;2-GFP protein in the endodermis of roots in the wild-type seedlings increased during dark adaptation, but not in the phyA mutant. The amount of the TIP2;2-GFP protein in wild-type seedlings decreased rapidly under far-red light illumination, and a delay in reduction of TIP2;2-GFP was observed in the phyA mutant. Our results imply that phyA, cooperating with other photoreceptors, modulates the level of TIP2;2 in Arabidopsis roots.

Keywords: Arabidopsis thaliana; aquaporin; dark adaptation; endodermis; light regulation; phytochrome; root; water transport

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MDPI and ACS Style

Uenishi, Y.; Nakabayashi, Y.; Tsuchihira, A.; Takusagawa, M.; Hashimoto, K.; Maeshima, M.; Sato-Nara, K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants 2014, 3, 177-195. https://doi.org/10.3390/plants3010177

AMA Style

Uenishi Y, Nakabayashi Y, Tsuchihira A, Takusagawa M, Hashimoto K, Maeshima M, Sato-Nara K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants. 2014; 3(1):177-195. https://doi.org/10.3390/plants3010177

Chicago/Turabian Style

Uenishi, Yumi, Yukari Nakabayashi, Ayako Tsuchihira, Mari Takusagawa, Kayo Hashimoto, Masayoshi Maeshima, and Kumi Sato-Nara. 2014. "Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings" Plants 3, no. 1: 177-195. https://doi.org/10.3390/plants3010177

APA Style

Uenishi, Y., Nakabayashi, Y., Tsuchihira, A., Takusagawa, M., Hashimoto, K., Maeshima, M., & Sato-Nara, K. (2014). Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants, 3(1), 177-195. https://doi.org/10.3390/plants3010177

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Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings

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