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Volume 7
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10.3390/plants7020034
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Communication

TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax

by
Yong-Bi Fu
*,
Zaheer Ahmed
,
Hui Yang
and
Carolee Horbach
Plant Gene Resources of Canada, Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
*
Author to whom correspondence should be addressed.
Plants 2018, 7(2), 34; https://doi.org/10.3390/plants7020034
Submission received: 20 February 2018 / Revised: 4 April 2018 / Accepted: 11 April 2018 / Published: 13 April 2018
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Abstract

In a search for useful seed aging signals as biomarkers for seed viability prediction, we conducted an experiment using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining to analyze morphological and molecular changes in naturally aged (NA) and artificially aged (AA) flax (Linum usitatissimum L.) seeds. A total of 2546 sections were performed from 112 seeds of 12 NA and AA seed samples with variable germination rates. Analyzing 1384 micrographs generated from TUNEL assay and DAPI staining revealed few alterations of the cellular morphology of the NA and AA seeds. Also, the revealed DNA degradations in the aged flax seeds appeared to be associated with seed samples of low germination rates. These results suggest that oily flax seed aging may alter the cellular morphology differently than starchy wheat seed aging. The results also imply that the TUNEL assay and DAPI staining may not yield informative assessments on cellular alterations and DNA degradation after the aging of oily seeds.
Keywords: flax; seed viability; seed aging; TUNEL; DAPI; seed cellular morphology flax; seed viability; seed aging; TUNEL; DAPI; seed cellular morphology

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MDPI and ACS Style

Fu, Y.-B.; Ahmed, Z.; Yang, H.; Horbach, C. TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax. Plants 2018, 7, 34. https://doi.org/10.3390/plants7020034

AMA Style

Fu Y-B, Ahmed Z, Yang H, Horbach C. TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax. Plants. 2018; 7(2):34. https://doi.org/10.3390/plants7020034

Chicago/Turabian Style

Fu, Yong-Bi, Zaheer Ahmed, Hui Yang, and Carolee Horbach. 2018. "TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax" Plants 7, no. 2: 34. https://doi.org/10.3390/plants7020034

APA Style

Fu, Y.-B., Ahmed, Z., Yang, H., & Horbach, C. (2018). TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax. Plants, 7(2), 34. https://doi.org/10.3390/plants7020034

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