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Volume 8
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10.3390/plants8120567
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Article

Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor

by
Carole Lambert
1,2,*,†,
Julien Lemaire
2,
Hélène Auger
2,
Arnaud Guilleret
1,
Romain Reynaud
1,
Christophe Clément
3,
Eric Courot
3 and
Behnam Taidi
2
1
Givaudan France SAS, Active Beauty, 51110 Pomacle, France
2
LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 51110 Pomacle, France
3
Résistance Induite et Bioprotection des Plantes, EA 4707, SFR Condorcet FR CNRS 3417, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP1039, 51687 Reims CEDEX 02, France
*
Author to whom correspondence should be addressed.
†
Current position: Givaudan France SAS, Active Beauty, 51110 Pomacle, France.
Plants 2019, 8(12), 567; https://doi.org/10.3390/plants8120567
Submission received: 31 October 2019 / Revised: 22 November 2019 / Accepted: 29 November 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Resveratrol in Plants)
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Abstract

Resveratrol and its oligomers are biologically active compounds. This work brings new insights for the bioproduction of trans-resveratrol with three dimers, pallidol, trans-ε-viniferin, and trans-δ-viniferin, in cell suspension of Vitis labrusca. Conditions of elicitation by methyl jasmonate were optimized for the production of stilbenes using statistical design of experiment. Bio-production of stilbenes was scaled-up to 5 L and in these conditions, trans-resveratrol concentrations reached 237 mg/L, and for pallidol 114 mg/L. The comparison of different elicitation modes (different elicitors, combination with cyclodextrins or adsorbent resin) allowed to reach particularly high concentrations of target molecules: Resveratrol 6.14 g/L, pallidol 0.90 g/L, δ-viniferin 0.54 g/L, and ε-viniferin 0.50 g/L. Scale-up to 20 L-stirring-bioreactor gave similar growth rates to those observed in shake flask culture, with a high production of resveratrol (4.23 g/L) and δ-viniferin (0.76 g/L). This work provides new strategies for the production of stilbenes in plant cell suspension for biological and commercial evaluation.
Keywords: plant cell culture; resveratrol; stilbene; viniferins; elicitation; methyl jasmonate; methyl-β-cyclodextrin; Vitis labrusca; bioreactors plant cell culture; resveratrol; stilbene; viniferins; elicitation; methyl jasmonate; methyl-β-cyclodextrin; Vitis labrusca; bioreactors

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MDPI and ACS Style

Lambert, C.; Lemaire, J.; Auger, H.; Guilleret, A.; Reynaud, R.; Clément, C.; Courot, E.; Taidi, B. Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor. Plants 2019, 8, 567. https://doi.org/10.3390/plants8120567

AMA Style

Lambert C, Lemaire J, Auger H, Guilleret A, Reynaud R, Clément C, Courot E, Taidi B. Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor. Plants. 2019; 8(12):567. https://doi.org/10.3390/plants8120567

Chicago/Turabian Style

Lambert, Carole, Julien Lemaire, Hélène Auger, Arnaud Guilleret, Romain Reynaud, Christophe Clément, Eric Courot, and Behnam Taidi. 2019. "Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor" Plants 8, no. 12: 567. https://doi.org/10.3390/plants8120567

APA Style

Lambert, C., Lemaire, J., Auger, H., Guilleret, A., Reynaud, R., Clément, C., Courot, E., & Taidi, B. (2019). Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor. Plants, 8(12), 567. https://doi.org/10.3390/plants8120567

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