Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens
Abstract
:1. Introduction
2. Results and Discussion
2.1. Diminished Water Content of jai1-1 Is Restored by Ethylene Insensitivity
2.2. Diminished Pollen Vitality of Jai1-1 Is Partially Restored by Ethylene Insensitivity
2.3. Hormone Levels in Developing Stamen
2.4. Accumulation of Phenylpropanoids
2.5. Transcript Accumulation of JA and ET Related Genes
3. Materials and Methods
3.1. Plant Material, Growth Conditions and Harvest of Stamen
3.2. Determination of Water Content of Stamen and Pollen Vitality
3.3. Determination of ACC, JA and JA-Ile
3.4. Extraction of Metabolites and Separation by High Performance Thin-Layer-Chromatography (HPTLC) and Liquid Chromatography-Mass Spectroscopy (LC-MS)
3.5. Preparation of the Feruloylquinic Acid Standard
3.6. Isolation of RNA and RT-qPCR based on Nanofluidic Arrays
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Schubert, R.; Grunewald, S.; von Sivers, L.; Hause, B. Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens. Plants 2019, 8, 277. https://doi.org/10.3390/plants8080277
Schubert R, Grunewald S, von Sivers L, Hause B. Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens. Plants. 2019; 8(8):277. https://doi.org/10.3390/plants8080277
Chicago/Turabian StyleSchubert, Ramona, Stephan Grunewald, Lea von Sivers, and Bettina Hause. 2019. "Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens" Plants 8, no. 8: 277. https://doi.org/10.3390/plants8080277