Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method
Abstract
:1. Introduction
2. Results
2.1. Comparison of the Plant Growth Characteristics of SS and CS-Derived Plants
2.2. Multivariate Statistical Analysis and Component Analysis Using Fourier Transform Near-Infrared (FT-NIR) Spectrophotometry and Liquid Chromatography-Mass Spectrometry (LC-MS), Respectively
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Transplanting, and Harvesting
4.2. Assessment of the Underground Polygonum Multiflorum Parts
4.3. FT-NIR Analysis and Quality Inspection
4.4. Extraction
4.5. LC-MS Analysis for Bioequivalence Validation
4.6. Quantitative Analysis of TSG
4.7. Data Recording and Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Compound | 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside (TSG) | Samples | Contents of TSG (μg/mg) c |
---|---|---|---|
R2 a LOD (μg/mg) b | 0.997 0.073 | SS CS | 50.75 (5.075%) 53.59 (5.359%) |
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Kim, Y.-G.; Komakech, R.; Jeong, D.H.; Jeon, K.; Park, Y.; Lee, T.K.; Kim, K.H.; Moon, B.C.; Kang, Y. Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method. Plants 2020, 9, 1280. https://doi.org/10.3390/plants9101280
Kim Y-G, Komakech R, Jeong DH, Jeon K, Park Y, Lee TK, Kim KH, Moon BC, Kang Y. Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method. Plants. 2020; 9(10):1280. https://doi.org/10.3390/plants9101280
Chicago/Turabian StyleKim, Yong-Goo, Richard Komakech, Dae Hui Jeong, Kwonseok Jeon, Yunmi Park, Tae Kyoung Lee, Ki Hyun Kim, Byeong Cheol Moon, and Youngmin Kang. 2020. "Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method" Plants 9, no. 10: 1280. https://doi.org/10.3390/plants9101280