Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants
Abstract
:1. Introduction
2. Results
2.1. Indicator for Microspore Stage
2.2. Callus Induction
2.3. Shoot Regeneration
2.4. Rooting and Acclimatization
2.5. Ploidy Evaluation
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Anther Culture
4.3. Acclimatization
4.4. Ploidy-Status Assessment in Regenerants
4.5. STMS Marker Analysis
4.6. Observations and Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Seidelmann, S.B.; Claggett, B.; Cheng, S.; Henglin, M.; Shah, A.; Steffen, L.M.; Folsom, A.R.; Rimm, E.B.; Willett, W.C.; Solomon, S.D. Dietary carbohydrate intake and mortality: A prospective cohort study and meta-analysis. Lancet Public Health 2018, 3, e419–e428. [Google Scholar] [PubMed]
- IRRI. Available online: http://ricestat.irri.org/vis/wrs_quickCharts.phpReturn (accessed on 22 April 2012).
- Yuan, L.P. Increasing yield potential in rice by exploitation of heterosis. In Hybrid Rice Technology: New Developments and Future Prospects; Virmani, S.S., Ed.; IRRI: Manila, Philippines, 1994; pp. 1–6. [Google Scholar]
- Rout, P.; Naik, N.; Ngangkham, U.; Verma, R.L.; Katara, J.L.; Singh, O.N.; Samantaray, S. Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization. Plant Biotechnol. J. 2016, 33, 177–186. [Google Scholar] [CrossRef]
- Naik, N.; Rout, P.; Umakanta, N.; Verma, R.L.; Katara, J.L.; Sahoo, K.K.; Singh, O.N.; Samantaray, S. Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell Tissue Organ Cult. 2017, 128, 679–689. [Google Scholar] [CrossRef]
- Grewal, D.; Manito, C.H.; Bartolome, V. Doubled haploids generated through anther culture from crosses of elite indica and japonica cultivars and/or lines of rice: Large scale production, agronomic performance and molecular characterization. Crop Sci. 2011, 51, 2544–2553. [Google Scholar] [CrossRef]
- Dewi, I.S.; Purwoko, B.S.; Aswidinnoor, H.; Somantri, I.H.; Chozin, M.A. Plant regeneration from anther cultures of several genotypes of indica rice tolerant to aluminium toxicity. Ind. J. Agric. Sci. 2009, 2, 1–5. [Google Scholar]
- Guzman, M.; Arias, F.J.Z. Increasing anther culture efficiency in rice(Oryza sativa L.) using anthers from ratooned plants. Plant Sci. 2000, 151, 107–114. [Google Scholar] [CrossRef]
- Huang, X.; Kurata, N.; Wang, Z.X.; Wang, A.; Zhao, Q.; Zhao, Y.; Liu, K.; Lu, H.; Li, W.; Guo, Y.; et al. A map of rice genome variation reveals the origin of cultivated rice. Nature 2012, 490, 497–501. [Google Scholar] [CrossRef] [PubMed]
- Talebi, R.; Rahemi, M.R.; Arefi, H.; Nourozi, M.; Bagheri, N. In vitro plant regeneration through anther culture of some Iranian local rice (Oryza sativa L.) cultivars. Pak. J. Biological. Sci. 2007, 10, 2056–2060. [Google Scholar] [CrossRef]
- Raina, S.K.; Zapata, F.J. Enhanced anther culture efficiency of indica rice (Oryza sativa L.) through modification of the culture media. Plant Breed. 1997, 116, 305–315. [Google Scholar] [CrossRef]
- Zapata, F.J.; Alejar, M.S.; Torrizo, L.B.; Novero, A.U.; Singh, V.P.; Senadhira, D. Field performance of anther-culture-derived lines from F 1 crosses of Indica rices under saline and nonsaline conditions. Theor. Appl. Genet 1991, 83, 6–11. [Google Scholar] [CrossRef]
- Chen, T.; Zhang, Y.; Zhao, L.; Zhu, Z.; Lin, J.; Zhang, S.; Wang, C. Fine mapping and candidate gene analysis of a green-revertible albino gene gra (t) in rice. J. Genet. Genom. 2009, 36, 117–123. [Google Scholar] [CrossRef]
- Sharma, S.; Sarkar, D.; Pandey, S.K. Phenotypic characterization and nuclear microsatellite analysis reveal genomic changes and rearrangements underlying androgenesis in tetraploid potatoes (Solanum tuberosum L.). Euphytica 2010, 171, 313. [Google Scholar] [CrossRef]
- Ahmadi, B.; Ebrahimzadeh, H. In vitro androgenesis: Spontaneous vs. artificial genome doubling and characterization of regenerants. Plant Cell Rep. 2020, 39, 299–316. [Google Scholar] [CrossRef] [PubMed]
- Murashige, T.P.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962, 15, 473–497. [Google Scholar] [CrossRef]
- Chu, C.C.; Wang, C.C.; Sun, C.S.; Hsu, C.; Yin, K.C.; Chu, Y.C.; Bi, F.Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 1975, 85, 659–668. [Google Scholar] [CrossRef]
- Murray, M.G.; Thompson, W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980, 8, 4321–4326. [Google Scholar] [CrossRef] [PubMed]
Explant Source | Days of Pre-Treatment | Callus Response in “N6” Media * | Callus Response in “MS” Media * | ||||
---|---|---|---|---|---|---|---|
N-1 | N-2 | N-3 | M-1 | M-2 | M-3 | ||
Non ratooned | 2 | 21.66 ± 0.75 a | 9.00 ± 0.17 a | 2.26 ± 0.15 a | 13.66 ± 0.05 a | 9.66 ± 0.15 a | 7.10 ± 0.43 a |
7 | 14.33 ± 0.35 b | 3.96 ± 0.35 b | 1.50 ± 0.43 b | 8.53 ± 0.35 b | 5.00 ± 0.52 b | 3.73 ± 0.32 b | |
8 | 10.00 ± 0.26 d | 1.93 ± 0.32 d | 0.86 ± 0.30 d | 5.83 ± 0.20 c | 3.00 ± 0.30 c | 2.70 ± 0.45 c | |
Ratooned | 2 | 11.66 ± 0.30 c | 2.66 ± 0.15 c | 1.20 ± 0.20 c | 2.66 ± 0.40 d | 0.63 ± 0.15 d | 0.33 ± 0.15 d |
7 | 6.67 ± 0.32 e | 0.66 ± 0.15 e | 0.30 ± 0.26 e | 1.33 ± 0.15 e | 0.10 ± 0.10 e | 0 | |
8 | 2.66 ± 0.20 f | 0.36 ± 0.35 f | 0 | 0.66 ± 0.41 f | 0 | 0 |
Source of Explant | Calli Induction Media | Green Shoot Regeneration (%) in MS Media + Growth Regulators * | Source of Explant | Calli Induction Media | Green Shoot Regeneration (%) in MS Media + Growth Regulators * | ||
---|---|---|---|---|---|---|---|
MS1 | MS2 | MS1 | MS2 | ||||
Non-ratooned | N1 | 76.00 ± 0.30 a | 27.66 ± 0.15 a | Ratooned | N1 | 38.86 ± 0.26 a | 19.96 ± 0.25 a |
N2 | 37.66 ± 0.15 b | 14.33 ± 0.14 d | N2 | 18.16 ± 0.14 b | 13.40 ± 0.17 b | ||
N3 | 29.33 ± 0.35 c | 11.66 ± 0.25 e | N3 | 13.16 ± 0.13 c | 13.00 ± 0.33 bc | ||
M1 | 22.33 ± 0.37 d | 20.13 ± 0.35 b | M1 | 10.63 ± 0.30 e | 12.53 ± 0.35 d | ||
M2 | 15.33 ± 0.25 e | 16.66 ± 0.05 c | M2 | 12.53 ± 0.25 d | 11.30 ± 0.37 e | ||
M3 | 13.33 ± 0.30 f | 0 | M3 | 0 | 0 |
Basal Media + Growth Regulators | Media Combinations | 2,4-D (mg/L) | BAP (mg/L) | Kn (mg/L) |
---|---|---|---|---|
N6 media | N1 | 2.0 | 0.5 | -- |
N2 | 1.5 | -- | 0.1 | |
N3 | 2.5 | 1.0 | -- | |
MS media | M1 | 2.0 | 0.5 | 0.1 |
M2 | 2.0 | -- | 0.1 | |
M3 | 2.5 | 1.0 | -- |
Semi-Solid MS Media | BAP (mg/L) | Kn (mg/L) | NAA (mg/L) |
---|---|---|---|
MS1 | 1.5 | 0.5 | 0.5 |
MS2 | 2.0 | 1.0 | 0.5 |
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Pattnaik, S.S.; Dash, B.; Bhuyan, S.S.; Katara, J.L.; Parameswaran, C.; Verma, R.; Ramesh, N.; Samantaray, S. Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants. Plants 2020, 9, 1306. https://doi.org/10.3390/plants9101306
Pattnaik SS, Dash B, Bhuyan SS, Katara JL, Parameswaran C, Verma R, Ramesh N, Samantaray S. Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants. Plants. 2020; 9(10):1306. https://doi.org/10.3390/plants9101306
Chicago/Turabian StylePattnaik, Snigdha Samir, Byomkesh Dash, Sudhansu Sekhar Bhuyan, Jawahar Lal Katara, C. Parameswaran, Ramlakhan Verma, Narayanaperumal Ramesh, and Sanghamitra Samantaray. 2020. "Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants" Plants 9, no. 10: 1306. https://doi.org/10.3390/plants9101306