Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare

Aiello, Domenico; Ferradini, Nicoletta; Torelli, Lorenzo; Volpi, Chiara; Lambalk, Joep; Russi, Luigi; Albertini, Emidio

doi:10.3390/plants9020175

Open AccessArticle

Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare

by

Domenico Aiello

¹

,

Nicoletta Ferradini

¹,

Lorenzo Torelli

¹,

Chiara Volpi

²,

Joep Lambalk

³,

Luigi Russi

¹

and

Emidio Albertini

^1,*

¹

Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy

²

Enza Zaden Italia Research S.r.l. SS., 01016 Tarquinia, Italy

³

Enza Zaden, Research and Development B.V. P.O. Box 7, 1600AA Enkhuizen, The Netherlands

^*

Author to whom correspondence should be addressed.

Plants 2020, 9(2), 175; https://doi.org/10.3390/plants9020175

Submission received: 5 December 2019 / Revised: 30 December 2019 / Accepted: 28 January 2020 / Published: 1 February 2020

(This article belongs to the Special Issue Genomics for Plant Breeding)

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Abstract

Fennel (Foeniculum vulgare) is a species belonging to the Apiaceae family, well known for its nutritional and pharmacological properties. Despite the economic and agricultural relevance, its genomic and transcriptomic data remain poor. Microsatellites—also known as simple sequence repeats (SSRs)—are codominant markers widely used to perform cross-amplification tests starting from markers developed in related species. SSRs represent a powerful tool, especially for those species lacking genomic information. In this study, a set of primers previously designed in Daucus carota for polymorphic SSR loci was tested in commercial varieties and breeding lines of fennel in order to: (i) test their cross-genera transferability, (ii) look at their efficiency in assessing genetic diversity, and (iii) identify their usefulness for marker-assisted selection (MAS) in breeding programs. Thirty-nine SSR markers from carrot were selected and tested for their transferability score, and only 23% of them resulted suitable for fennel. The low rate of SSR transferability between the two species evidences the difficulties of the use of genomic SSR in cross-genera transferability.

Keywords: Foeniculum vulgare; fennel; Daucus carota; SSR; cross-species

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MDPI and ACS Style

Aiello, D.; Ferradini, N.; Torelli, L.; Volpi, C.; Lambalk, J.; Russi, L.; Albertini, E. Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare. Plants 2020, 9, 175. https://doi.org/10.3390/plants9020175

AMA Style

Aiello D, Ferradini N, Torelli L, Volpi C, Lambalk J, Russi L, Albertini E. Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare. Plants. 2020; 9(2):175. https://doi.org/10.3390/plants9020175

Chicago/Turabian Style

Aiello, Domenico, Nicoletta Ferradini, Lorenzo Torelli, Chiara Volpi, Joep Lambalk, Luigi Russi, and Emidio Albertini. 2020. "Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare" Plants 9, no. 2: 175. https://doi.org/10.3390/plants9020175

APA Style

Aiello, D., Ferradini, N., Torelli, L., Volpi, C., Lambalk, J., Russi, L., & Albertini, E. (2020). Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare. Plants, 9(2), 175. https://doi.org/10.3390/plants9020175

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Evaluation of Cross-Species Transferability of SSR Markers in Foeniculum vulgare

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