Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes
Abstract
:1. Introduction
2. Results
2.1. General Responses
2.2. CIM Browning Responses
2.3. RM Responses
2.4. Performance Following ERM Culture
3. Discussion
3.1. In Vitro Performance
3.2. Medium Browning
3.3. Albino Regeneration
3.4. Summary
4. Materials and Methods
4.1. Plant Materials
4.2. Tissue Culture Media
4.3. Explant Culture
4.4. Tissue Culture Data Collection
4.5. Image and Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genotype | Protocol | Proliferation Index | Medium Browning |
---|---|---|---|
Tx430 | LG | ++++ | - |
WU | +++ | + | |
Atlas | LG | ++ | + |
WU | +++ | ++ | |
Grassl | LG | ++ | +++ |
WU | +++ | +++ | |
Rio | LG | ++ | + |
WU | +++ | ++++ | |
Pink Kafir | LG | +++ | + |
WU | ++++ | ++++ | |
PI329311 | LG | ++++ | + |
WU | ++++ | ++ | |
Leoti | LG | + | ++ |
WU | + | ++ | |
Chinese Amber | LG | + | +++ |
WU | + | +++ |
Genotype (Name and/or Identifier) | Sorghum Type | Seed Color/Tannin | Plant Color | Race | Origin Location |
---|---|---|---|---|---|
Tx430 | Grain | Yellow/no | Purple | Caudatum | USA |
Atlas (PI641807) | Sweet | White/no | Purple | Kafir-Bicolor | USA |
Grassl | Cellulosic | Brown/yes | Purple | Caudatum | Uganda |
Rio | Sweet | White/yes | Purple | Caudatum | USA |
Pink Kafir (PI655972) | Grain | Red-white/no | Purple | Kafir | USA |
PI329311 | Cellulosic | Yellow/no | Purple | Durra | Ethiopia |
Leoti (PI586454) | Sweet | Brown/yes | Purple | Kafir | Hungary |
Chinese Amber (PI22913) | Sweet | Brown/yes | Purple | Bicolor | China |
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Flinn, B.; Dale, S.; Disharoon, A.; Kresovich, S. Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes. Plants 2020, 9, 248. https://doi.org/10.3390/plants9020248
Flinn B, Dale S, Disharoon A, Kresovich S. Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes. Plants. 2020; 9(2):248. https://doi.org/10.3390/plants9020248
Chicago/Turabian StyleFlinn, Barry, Savanah Dale, Andrew Disharoon, and Stephen Kresovich. 2020. "Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes" Plants 9, no. 2: 248. https://doi.org/10.3390/plants9020248