The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Identification of Sprt Mutants
2.2. Most rfc3 Mutant Phenotypes were Recovered by the sprt2-1 Mutation
2.3. rfc3-2 sprt2-1 Mutants Exhibit Restoration of Plastid rRNA Accumulation
2.4. Effect of the sprt2-1 Mutation on Plastid Ribosome Biogenesis
2.5. Identification of the sprt2-1 Mutation Site
2.6. Expression Pattern of CFM3b and Subcellular Localization of its Product
2.7. RFC3 Interacts with CFM3b in Plastids
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Isolation of Suppressor Mutants of rfc3-2
4.3. Genetic Mapping of the sprt2-1 Mutation Site
4.4. Complementation Test of sprt2
4.5. Microscopy
4.6. Transient Assays
4.7. Northern Blot Analysis of Plastid rRNAs
4.8. RT-qPCR
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nagashima, Y.; Ohshiro, K.; Iwase, A.; Nakata, M.T.; Maekawa, S.; Horiguchi, G. The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana. Plants 2020, 9, 328. https://doi.org/10.3390/plants9030328
Nagashima Y, Ohshiro K, Iwase A, Nakata MT, Maekawa S, Horiguchi G. The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana. Plants. 2020; 9(3):328. https://doi.org/10.3390/plants9030328
Chicago/Turabian StyleNagashima, Yumi, Katsutomo Ohshiro, Akiyasu Iwase, Miyuki T. Nakata, Shugo Maekawa, and Gorou Horiguchi. 2020. "The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana" Plants 9, no. 3: 328. https://doi.org/10.3390/plants9030328