Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening
Abstract
:1. Introduction
2. Results
2.1. Transcriptomic Analysis of the Effects of A9 and A4a
2.2. A9 Enhances Responses to Blue Light Mediated by CRY1
2.3. Comparative Loss-of-Function Effects of A9 in Seed Longevity and Seedling Greening
3. Discussion
4. Materials and Methods
4.1. Plant Material and Transgenic Lines
4.2. Promoter Activation Assays
4.3. RNA-Seq Transcript Analysis
4.4. Protein Accumulation Gel Blot Analyses
4.5. Seed Deterioration
4.6. Light Treatments and Quantification of Hypocotyl Growth Reduction and Seedling Greening
4.7. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Almoguera, C.; Prieto-Dapena, P.; Carranco, R.; Ruiz, J.L.; Jordano, J. Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening. Plants 2020, 9, 335. https://doi.org/10.3390/plants9030335
Almoguera C, Prieto-Dapena P, Carranco R, Ruiz JL, Jordano J. Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening. Plants. 2020; 9(3):335. https://doi.org/10.3390/plants9030335
Chicago/Turabian StyleAlmoguera, Concepción, Pilar Prieto-Dapena, Raúl Carranco, José Luis Ruiz, and Juan Jordano. 2020. "Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening" Plants 9, no. 3: 335. https://doi.org/10.3390/plants9030335