Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia
Abstract
:1. Introduction
2. Results
2.1. Genetic Diversity of Olive Genotypes
2.2. Genetic Relationships Among Olive Genotypes
2.3. Genetic Structure
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. DNA Extraction
4.3. SSR Assays
4.4. Data Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Locus | Size Range (bp) | Na | Ne | I | Ho | He | F | PIC |
---|---|---|---|---|---|---|---|---|
DCA03 | 231–255 | 10 | 5.89 | 1.91 | 0.92 | 0.83 | −0.10 | 0.80 |
DCA05 | 194–212 | 7 | 3.04 | 1.44 | 0.77 | 0.67 | −0.16 | 0.64 |
DCA09 | 162–206 | 13 | 5.94 | 2.09 | 0.85 | 0.83 | −0.03 | 0.81 |
DCA15 | 246–270 | 4 | 1.84 | 0.88 | 0.39 | 0.45 | 0.14 | 0.42 |
DCA16 | 122–186 | 19 | 9.26 | 2.51 | 0.97 | 0.89 | −0.09 | 0.88 |
DCA17 | 109–181 | 9 | 3.11 | 1.39 | 0.25 | 0.67 | 0.62 | 0.63 |
DCA18 | 165–191 | 10 | 4.76 | 1.76 | 0.61 | 0.79 | 0.21 | 0.76 |
GAPU71b | 121–144 | 5 | 4.91 | 1.60 | 0.89 | 0.79 | −0.12 | 0.76 |
GAPU101 | 170–218 | 9 | 6.75 | 1.96 | 0.97 | 0.85 | −0.14 | 0.83 |
UDO28 | 115–169 | 17 | 6.77 | 2.20 | 0.79 | 0.85 | 0.07 | 0.83 |
UDO43 | 166–216 | 15 | 7.03 | 2.19 | 0.90 | 0.85 | −0.06 | 0.84 |
EMOL | 190–228 | 6 | 2.70 | 1.18 | 0.45 | 0.63 | 0.27 | 0.57 |
Total | 124 | 62.00 | ||||||
Mean | 10.33 | 5.16 | 1.76 | 0.73 | 0.76 | 0.05 | 0.73 |
Collections | Na | Ne | Ho | He | F | |
---|---|---|---|---|---|---|
Reference (IO) | Total | 92.0 | 55.7 | |||
Mean | 6.4 | 4.21 | 0.67 | 0.70 | 0.078 | |
Nurseries (GR) | Total | 77.0 | 50.5 | |||
Mean | 6.4 | 4.21 | 0.67 | 0.70 | 0.078 | |
Ras Jbal (RJ) | Total | 74.0 | 44.5 | |||
Mean | 6.1 | 3.71 | 0.82 | 0.67 | −0.219 | |
Azmour (AZ) | Total | 82.0 | 55.5 | |||
Mean | 6.8 | 4.63 | 0.79 | 0.73 | −0.066 |
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Saddoud Debbabi, O.; Miazzi, M.M.; Elloumi, O.; Fendri, M.; Ben Amar, F.; Savoia, M.; Sion, S.; Souabni, H.; Mnasri, S.R.; Ben Abdelaali, S.; et al. Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia. Plants 2020, 9, 382. https://doi.org/10.3390/plants9030382
Saddoud Debbabi O, Miazzi MM, Elloumi O, Fendri M, Ben Amar F, Savoia M, Sion S, Souabni H, Mnasri SR, Ben Abdelaali S, et al. Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia. Plants. 2020; 9(3):382. https://doi.org/10.3390/plants9030382
Chicago/Turabian StyleSaddoud Debbabi, Olfa, Monica Marilena Miazzi, Olfa Elloumi, Mahdi Fendri, Fathi Ben Amar, Michele Savoia, Sara Sion, Hana Souabni, Sameh Rahmani Mnasri, Selma Ben Abdelaali, and et al. 2020. "Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia" Plants 9, no. 3: 382. https://doi.org/10.3390/plants9030382