Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb
Abstract
:1. Introduction
2. Results
2.1. P. acinosa Showed High Potassium Characteristics
2.2. P. acinosa Shows Adaptability to a Wide Range of External Potassium Levels
2.3. Global Transcriptome Analysis Reveals the Involvement of Multiple Biological Processes during Potassium Accumulation
2.4. Expression Profile of K+ Transporters Based on Transcriptome Sequence
2.5. Expression Pattern of Potassium Transport Genes in P. acinosa
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Collection from Fields
5.2. Plant Materials and Growth Conditions
5.3. K+ Treatment for Physiological Tests
5.4. K+ Treatment for Transcriptome Sequencing
5.5. Measurement of Chlorophyll Content and Photosynthetic Rate in Leaves
5.6. K+ Content Analysis through ICP–MS
5.7. Transcriptome Analysis
5.8. Real-Time Quantitative PCR Analysis
5.9. Gene Searching and Phylogenetic Analysis
5.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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RawData | TotalReads | TotalBases | Error% | Q20% | Q30% | GC% |
---|---|---|---|---|---|---|
TP01-1 | 34,165,110 | 4,881,892,216 | 0.0134 | 97.5 | 91.74 | 51.45 |
TP01-2 | 33,169,330 | 4,878,900,814 | 0.0138 | 97.3 | 91.73 | 51.40 |
TP01-3 | 35,157,926 | 4,881,996,121 | 0.0136 | 97.4 | 91.78 | 51.47 |
TP02-1 | 75,835,214 | 10,521,500,724 | 0.0103 | 98.7 | 95.40 | 47.4 |
TP02-2 | 75,831,068 | 10,516,553,023 | 0.0101 | 98.2 | 95.27 | 46.9 |
TP02-3 | 75,834,548 | 10,520,436,458 | 0.0102 | 98.6 | 95.35 | 47.3 |
Parameter | Value | Parameter | Value |
---|---|---|---|
Radio frequency power | 1.45 V | Orifice of sampling cone | 1.0 mm |
Carrier gas flow rate | 0.85 L/min | Orifice of skimmer cone | 0.4 mm |
Mixed gas flow rate | 0.28 L/min | Sample depth | 7.0 mm |
Plasma gas flow rate | 15.0 L/min | Sample uptake flow rate | 0.1 r/s |
Auxiliary gas flow rate | 1.0 L/min | Sample uptake amount | 0.4 mL/min |
Helium flow rate | 5 mL/min | Spray chamber temperature | 2 °C |
Seq_ID | Gene Name | Primer(5’-3’) |
---|---|---|
c126889g1_i1 | PaHAK5 | P1 ATGGATCTCAGCAGGATGGG |
P2 TTCCATTGTCGTTTGCCCTG | ||
c219737g1_i1 | PaKT2 | P1 GAGCCTTCTGCCAAAC |
P2 CCATCTCCAACCACCA | ||
c56511g1_i1 | PaKT4 | P1 GGTCGGTGTACGAGGAT |
P2 TGAGGCTAATGTGAGGAA | ||
c123766g1_i1 | PaKUP6 | P1 CGGGACCCTCAAGAAG |
P2 GCCTATGCCACGGACT | ||
c125123g6_i1 | PaKUP7 | P1 TCTGAGGCTATGTTTGCCGA |
P2 GTAGCTGTCGTCATTGCTCG | ||
c108651g2_i1 | PaAKT1 | P1 TTCAGAAGGGTTGCAACAGC |
P2 AAATTCCACGGCTCCAGAGA | ||
c121941g1_i1 | PaAKT2 | P1 GGAGCAGGGAAATGTTGTGG |
P2 TAAGGCTGAGGGATGTTGCA | ||
c72107g1_i1 | PaHKT1 | P1 CGGTTCTTGTGCTGTT |
P2 TGCTTCTTGTCGCTGA | ||
Actin | P1 CTTGACTTTGAGCAGGAATCGGAGA | |
P2 ACCTGCTGCTTCCATACCTATCAAT |
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Xie, Q.; Ma, L.; Tan, P.; Deng, W.; Huang, C.; Liu, D.; Lin, W.; Su, Y. Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb. Plants 2020, 9, 470. https://doi.org/10.3390/plants9040470
Xie Q, Ma L, Tan P, Deng W, Huang C, Liu D, Lin W, Su Y. Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb. Plants. 2020; 9(4):470. https://doi.org/10.3390/plants9040470
Chicago/Turabian StyleXie, Qin, Liying Ma, Peng Tan, Wentao Deng, Chao Huang, Deming Liu, Wanhuang Lin, and Yi Su. 2020. "Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb" Plants 9, no. 4: 470. https://doi.org/10.3390/plants9040470