Ethylene is Involved in Symptom Development and Ribosomal Stress of Tomato Plants upon Citrus Exocortis Viroid Infection
Abstract
:1. Introduction
2. Results
2.1. Never Ripe Tomato Mutants Are Hyper-Susceptible to CEVd Infection
2.2. PR1 and ACS2 Are Highly Induced in Infected Nr Tomato Plants
2.3. Ethylene Production Is Increased in Nr Tomato Mutants upon CEVd Infection
2.4. Ribosomal Stress Is Enhanced in Never Ripe Tomato Mutants upon CEVd Infection
3. Discussion
4. Materials and Methods
4.1. Plant Material and Viroid Inoculation
4.2. Ethylene Measurements
4.3. RNA Preparation
4.4. RT-PCR and qRT-PCR
4.5. Northern Blot Hybridization
4.6. Protein Extraction and Electrophoresis Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
Actin | CTAGGGTGGGTTCGCAGGAGATGATGC | GTCTTTTTGACCCATACCCACCATCACAC |
PR1 | ACTCAAGTAGTCTGGCGCAACTCA | AGTAAGGACGTTGTCCGATCGAGT |
ACS2 | GATGGATTTGCGTCCACTTT | GATCCAGGCGAGACGTTAAG |
ACO1 | TGTCCTAAGCCCGATTTGAT | TTGAGGAGTTGAAGGCCACT |
CEVd | AGGAGCTCGTCTCCTTCCTT | CACCGGGTAGTAGCCAGAAG |
SlNAC082 | TGCTGAAACCATTGGAACTG | CCAAGGAATTGCTTCCAAAA |
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Vázquez Prol, F.; López-Gresa, M.P.; Rodrigo, I.; Bellés, J.M.; Lisón, P. Ethylene is Involved in Symptom Development and Ribosomal Stress of Tomato Plants upon Citrus Exocortis Viroid Infection. Plants 2020, 9, 582. https://doi.org/10.3390/plants9050582
Vázquez Prol F, López-Gresa MP, Rodrigo I, Bellés JM, Lisón P. Ethylene is Involved in Symptom Development and Ribosomal Stress of Tomato Plants upon Citrus Exocortis Viroid Infection. Plants. 2020; 9(5):582. https://doi.org/10.3390/plants9050582
Chicago/Turabian StyleVázquez Prol, Francisco, M. Pilar López-Gresa, Ismael Rodrigo, José María Bellés, and Purificación Lisón. 2020. "Ethylene is Involved in Symptom Development and Ribosomal Stress of Tomato Plants upon Citrus Exocortis Viroid Infection" Plants 9, no. 5: 582. https://doi.org/10.3390/plants9050582