Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. RNA Extraction and Sequencing
2.3. Sequence Assembly and Analysis
2.4. Mechanical Transmission to Herbaceous Host
3. Results
3.1. Sequencing Results and Viral Identification
3.2. Phylogenetic Placement of Identified Viruses
3.3. Epidemiological Analysis and Mechanical Inoculation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Country | Cultivar | GaPlV1-Infected/Total Samples |
---|---|---|
Italy | Bianco d’Alessano, Cesanese d’Affile, Chardonnay, Cornalin, Croatina, Glera, Gratena, Lambrusco Maestri, Lambrusco Salamino, Merlot, Nerello Mascalese, Pinot gris, Pinot noir, Primitivo, Traminer, Trebbiano toscano, Verdicchio, Vermentino | 21/28 |
Portugal | Albariño, Cannonau, Chardonnay, Fernão Pires, Godello, Malvasia fine, Tinta barroca, Touriga Franca | 1/10 |
Spain | Airen, Albariño, Fernão Pires, Macabeo, Parellada, Pedro Ximenez, Tinta barroca, Touriga Franca, Touriga National, Trepat | 8/10 |
France | Chardonnay, Pinot gris, Pinot noir | 5/10 |
Greece | Agiogirtiko, Assyrtiko, Kidonitsa, Kocifali, Roditis, Vilana | 7/10 |
Bulgaria | Chardonnay, Dymiat, Brestovitsa, Pamid | 1/4 |
Romania | Busuioaca de Bohotin, Galbena de Odobesti, Merlot, Rkatsiteli | 4/5 |
Hungary | Franconia, Furmint, Harslevelu | 2/5 |
Ukraine | Kokur Belji, Krasnostop zolotovsicij, Plecistik, Telebi koruk, Zimljansku cernj | 4/5 |
Croatia | Malvasia, Plavac mali, Teran | 4/4 |
Total | 64 | 57/91 |
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Bertazzon, N.; Chitarra, W.; Angelini, E.; Nerva, L. Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard. Plants 2020, 9, 835. https://doi.org/10.3390/plants9070835
Bertazzon N, Chitarra W, Angelini E, Nerva L. Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard. Plants. 2020; 9(7):835. https://doi.org/10.3390/plants9070835
Chicago/Turabian StyleBertazzon, Nadia, Walter Chitarra, Elisa Angelini, and Luca Nerva. 2020. "Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard" Plants 9, no. 7: 835. https://doi.org/10.3390/plants9070835