Comparative Metabolite Profiling of Wild and Cultivated Justicia procumbens L. Based on 1H-NMR Spectroscopy and HPLC-DAD Analysis
Abstract
:1. Introduction
2. Results
2.1. HPLC Analysis and Peak Identification
2.2. 1H-NMR Analysis
2.3. Multivariate Analysis
2.3.1. Multivariate Analysis of HPLC-DAD Data
2.3.2. Multivariate Analysis of 1H-NMR Data
3. Discussion
4. Materials and Methods
4.1. Plant Material Collection and Cultivation
4.2. Sample Preparation
4.3. HPLC-DAD Analysis and 1H NMR Analysis
4.3.1. HPLC-DAD Analysis
4.3.2. 1H-NMR Spectra Acquisition
4.4. Multivariate Data Processing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample No. | Content (mg/g, n = 3) | Sample No. | Content (mg/g, n = 3) | Sample No. | Content (mg/g, n = 3) |
---|---|---|---|---|---|
S1 | 1.00 ± 0.04 | S13 | 0.38 ± 0.03 | S25 | 1.38 ± 0.08 |
S2 | 0.67 ± 0.00 | S14 | 0.45 ± 0.10 | S26 | 0.89 ± 0.09 |
S3 | 0.49 ± 0.01 | S15 | 0.33 ± 0.09 | S27 | 0.40 ± 0.02 |
S4 | 0.40 ± 0.01 | S16 | 0.28 ± 0.02 | S28 | 0.97 ± 0.23 |
S5 | 0.54 ± 0.12 | S17 # | 0.86 ± 0.11 | S29 | 0.46 ± 0.04 |
S6 | 0.58 ± 0.04 | S18 # | 0.54 ± 0.07 | S30 | 0.33 ± 0.02 |
S7 | 0.95 ± 0.04 | S19 # | 0.79 ± 0.08 | S31 | 0.59 ± 0.03 |
S8 | 0.22 ± 0.04 | S20 # | 1.21 ± 0.04 | S32 # | 1.12 ± 0.02 |
S9 | 0.51 ± 0.07 | S21 # | 0.88 ± 0.02 | S33 # | 1.03 ± 0.08 |
S10 | 0.74 ± 0.19 | S22 # | 0.75 ± 0.03 | S34 # | 0.40 ± 0.02 |
S11 | 1.13 ± 0.33 | S23 # | 0.84 ± 0.02 | S35 # | 0.64 ± 0.12 |
S12 | 0.69 ± 0.08 | S24 # | 0.53 ± 0.02 | - | - |
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Lee, H.; Jeon, J.; Yoon, J.; Kim, S.-H.; Choi, H.S.; Kang, J.S.; Lee, Y.S.; Lee, M.; Kim, Y.H.; Chang, H.B. Comparative Metabolite Profiling of Wild and Cultivated Justicia procumbens L. Based on 1H-NMR Spectroscopy and HPLC-DAD Analysis. Plants 2020, 9, 860. https://doi.org/10.3390/plants9070860
Lee H, Jeon J, Yoon J, Kim S-H, Choi HS, Kang JS, Lee YS, Lee M, Kim YH, Chang HB. Comparative Metabolite Profiling of Wild and Cultivated Justicia procumbens L. Based on 1H-NMR Spectroscopy and HPLC-DAD Analysis. Plants. 2020; 9(7):860. https://doi.org/10.3390/plants9070860
Chicago/Turabian StyleLee, Hyunyong, Jihyun Jeon, Joobyoung Yoon, Seung-Hwan Kim, Hyun Sik Choi, Jong Seung Kang, Yong Sup Lee, Mase Lee, Young Ho Kim, and Hwan Bong Chang. 2020. "Comparative Metabolite Profiling of Wild and Cultivated Justicia procumbens L. Based on 1H-NMR Spectroscopy and HPLC-DAD Analysis" Plants 9, no. 7: 860. https://doi.org/10.3390/plants9070860