Decorative Magnolia Plants: A Comparison of the Content of Their Biologically Active Components Showing Antimicrobial Effects
Abstract
:1. Introduction
2. Results
3. Conclusions
4. Experimental Section
4.1. Plant Material
4.2. Preparation of Extracts
4.3. RP-HPLC Analysis
4.4. Antimicrobial Activity of Extracts
- ∆Afraction—the OD difference at the beginning and at the 18th hour of the measurement of the antimicrobial samples,
- ∆Acontrol—the OD difference at the beginning and at the 18th hour of the measurement for the unaffected microorganism.
Author Contributions
Funding
Conflicts of Interest
References
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Fraction | RT a | Identified Substance | Main Mass Fragments (m/z) |
---|---|---|---|
1 | 6.3 min | unidentified | - |
2 | 44.1 min | honokiol | 209.0597; 224.0844; 250.0999 |
3 | 45.5 min | obovatol | 133.0657, 164.0478. 240.0775 |
4 | 46.0 min | magnolol | 223.0785; 245.0968, 247.1124 |
5 | 47.5 min | unidentified | - |
6 | 48.0 min | unidentified | - |
Plant | Part of Plant | Honokiol (mg·g−1 Dry Mass) | Obovatol (mg·g−1 Dry Mass) | Magnolol (mg·g−1 Dry Mass) | |
---|---|---|---|---|---|
Magnolia hybrid plants | MPR 0131 | L | 3.64 ± 0.04 | 0.68 ± 0.05 | 1.01 ± 0.08 |
FP | 7.25 ± 0.01 | 8.98 ± 0.2 | 2.95 ± 0.00 | ||
SP | 47.43 ± 7.11 | 92.13 ± 13.82 | 25.81 ± 3.87 | ||
MPR 0151 | L | 2.01 ± 0.02 | 0.90 ± 0.01 | 0.76 ± 0.01 | |
FP | 13.01 ± 1.95 | 24.59 ± 3.69 | 6.00 ± 0.90 | ||
SP | 35.95 ± 5.39 | 80.67 ± 12.10 | 18.79 ± 2.82 | ||
MPR 0271 | L | 0.39 ± 0.06 | 0.09 ± 0.01 | 0.21 ± 0.03 | |
FP | 13.04 ± 1.96 | 26.28 ± 3.94 | 5.91 ± 0.89 | ||
SP | 47.25 ± 7.09 | 115.57 ± 17.34 | 22.51 ± 3.38 | ||
MPR 1501 | L | 3.03 ± 0.01 | 1.85 ± 0.03 | 1.26 ± 0.01 | |
FP | 25.23 ± 3.78 | 37.38 ± 5.61 | 10.41 ± 1.56 | ||
SP | 55.34 ± 8.30 | 117.49 ± 17.62 | 28.24 ± 4.24 | ||
MPR 1511 | L | 8.26 ± 1.24 | 2.21 ± 0.33 | 2.07 ± 0.31 | |
FP | 13.09 ± 1.96 | 19.84 ± 2.98 | 6.49 ± 0.97 | ||
SP | 26.10 ± 3.91 | 60.43 ± 9.06 | 13.15 ± 1.97 | ||
MPR 1531 | L | 0.11 ± 0.02 | 0.06 ± 0.01 | 0.06 ± 0.01 | |
FP | 18.90 ± 2.84 | 22.86 ± 3.43 | 8.30 ± 1.24 | ||
SP | 17.13 ± 2.57 | 39.95 ± 5.99 | 10.54 ± 1.58 | ||
Magnolia parent plants | MOB 1511 | L | 0.70 ± 0.01 | 54.18 ± 2.76 | 5.55 ± 0.25 |
FP | 1.74 ± 0.26 | 271.63 ± 40.74 | 13.04 ± 1.96 | ||
SP | 4.70 ± 0.70 | 649.38 ± 97.41 | 30.99 ± 4.65 | ||
MTR 1531 | L | 191.62 ± 28.74 | 4.03 ± 0.60 | 53.61 ± 8.04 | |
FP | 252.26 ± 37.84 | 71.85 ± 10.78 | 83.16 ± 12.47 | ||
SP | 230.82 ± 34.62 | 269.31 ± 40.40 | 99.91 ± 14.99 |
Plant | Part of Plant | I (%) for Concentration of Extract | ||
---|---|---|---|---|
125 μg·mL−1 | 62.5 μg·mL−1 | |||
Magnolia hybrid plants | MPR 0131 | L | 63.3 ± 3.7 | 54.3 ± 1.4 |
FP | 98.3 ± 3.2 | 84.9 ± 1.8 | ||
SP | 100.0 ± 7.2 | 81.7 ± 2.1 | ||
MPR 0151 | L | 88.4 ± 2.2 | 81.4 ± 4.0 | |
FP | 96.1 ± 1.8 | 13.9 ± 5.0 | ||
SP | 97.2 ± 3.3 | 77.1 ± 2.5 | ||
MPR 0271 | L | 98.4 ± 1.8 | 62.5 ± 2.1 | |
FP | 97.0 ± 0.8 | 15.0 ± 4.7 | ||
SP | 95.9 ± 0.5 | 62.5 ± 4.0 | ||
MPR 1501 | L | 56.2 ± 1.2 | 57.1 ± 5.8 | |
FP | 84.5 ± 2.1 | 83.9 ± 2.4 | ||
SP | 93.6 ± 4.6 | 87.9 ± 7.9 | ||
MPR 1511 | L | 51.4 ± 3.0 | 53.5 ± 6.4 | |
FP | 42.9 ± 4.4 | 0.0 ± 0.0 | ||
SP | 96.6 ± 1.4 | 82.7 ± 4.0 | ||
MPR 1531 | L | 64.3 ± 3.6 | 34.5 ± 4.0 | |
FP | 100.0 ± 2.0 | 37.2 ± 9.6 | ||
SP | 94.1 ± 0.3 | 48.8 ± 4.1 | ||
Magnolia parent plants | MOB 1511 | L | 80.4 ± 17.7 | 58.4 ± 11.3 |
FP | 65.1 ± 3.5 | 41.8 ± 6.7 | ||
SP | 98.4 ± 2.9 | 100.0 ± 4.0 | ||
MTR 1531 | L | 66.6 ± 18.8 | 68.7 ± 3.8 | |
FP | 65.4 ± 3.9 | 100.0 ± 4.6 | ||
SP | 92.0 ± 6.7 | 68.6 ± 35.6 |
Plant | Part of Plant | IC50 (μg·mL−1) | MIC b (μg·mL−1) | |
---|---|---|---|---|
Magnolia hybrid plants | MPR 0131 | L | - | >125 |
FP | 56.6 ± 0.8 c | 125 ± 6.3 | ||
SP | 46.9 ± 2.0 c | 125 ± 6.3 | ||
MPR 0151 | L | 002D | 125 | |
FP | - | >125 | ||
SP | 57.6 ± 0.1 c | 125 ± 6.3 | ||
MPR 0271 | L | 62.9 ± 10.9 c | 125 ± 6.3 | |
FP | - | >125 | ||
SP | - | >125 | ||
MPR 1501 | L | - | >125 | |
FP | - | >125 | ||
SP | - | > 25 | ||
MPR 1511 | L | - | >125 | |
FP | - | >125 | ||
SP | - | >125 | ||
MPR 1531 | L | - | >125 | |
FP | 71.0 ± 5.4 c | 125 ± 6.3 | ||
SP | - | >125 | ||
Mgnolia parent plants | MOB 1511 | L | - | >125 |
FP | - | >125 | ||
SP | 40.1 ± 7.4 c | 62.5 ± 3.1 | ||
MTR 1531 | L | - | >125 | |
FP | 31.6 ± 0.2 c | 62.5 ± 3.1 | ||
SP | 60.2 ± 26.4 c | 62.5 ± 3.1 |
Time (min) | Methanol | 10 mM Formic Acid |
---|---|---|
2 | 5% | 95% |
45 | 90% | 10% |
50 | 100% | 0% |
60 | 100% | 0% |
65 | 5% | 95% |
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Lovecká, P.; Svobodová, A.; Macůrková, A.; Vrchotová, B.; Demnerová, K.; Wimmer, Z. Decorative Magnolia Plants: A Comparison of the Content of Their Biologically Active Components Showing Antimicrobial Effects. Plants 2020, 9, 879. https://doi.org/10.3390/plants9070879
Lovecká P, Svobodová A, Macůrková A, Vrchotová B, Demnerová K, Wimmer Z. Decorative Magnolia Plants: A Comparison of the Content of Their Biologically Active Components Showing Antimicrobial Effects. Plants. 2020; 9(7):879. https://doi.org/10.3390/plants9070879
Chicago/Turabian StyleLovecká, Petra, Alžběta Svobodová, Anna Macůrková, Blanka Vrchotová, Kateřina Demnerová, and Zdeněk Wimmer. 2020. "Decorative Magnolia Plants: A Comparison of the Content of Their Biologically Active Components Showing Antimicrobial Effects" Plants 9, no. 7: 879. https://doi.org/10.3390/plants9070879