Influence of Citrus Scion/Rootstock Genotypes on Arbuscular Mycorrhizal Community Composition under Controlled Environment Condition
Abstract
:1. Introduction
2. Results
2.1. Overall Citrus AMF Taxonomic Richness
2.2. Difference in α Diversity of AMF Community among Different Citrus Genotypes
2.3. Citrus Genotypes Shape the AMF Community Composition
2.4. Relative Abundance of AMF Species under Different Citrus Genotypes
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Materials and Sample Preparation
5.2. DNA Extraction, Library Construction and Sequencing
5.3. Data Analysis
5.4. Phylogenetic Analysis
5.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Phylum | OTUs | Tags |
---|---|---|
Glomeromycota | 87 (65.91%) | 613,408 (99.81%) |
Basidiomycota | 13 (9.85%) | 794 (0.13%) |
Ascomycota | 8 (6.06%) | 41 (0.01%) |
Chytridiomycota | 1 (0.76%) | 13 (0.00%) |
unclassified | 23 (17.42%) | 334 (0.05%) |
AMF Family | AMF Species | OTU | Tags |
---|---|---|---|
Glomeraceae (39 VT, corresponding to 474,991 clean reads, accounting for 99.81% of the total (475,917) clean reads against the MAARJAM database) | Glomus.Yamato08.A1_VTX00100 | 2 | 134,970 |
Glomus.acnaGlo2_VTX00155 | 3 | 71,418 | |
Glomus.Yamato09.A2_VTX00248 | 1 | 66,821 | |
Glomus.viscosum_VTX00063 | 8 | 37,975 | |
Glomus.Glo7_VTX00214 | 3 | 30,951 | |
Glomus.NF13_VTX00419 | 2 | 21,670 | |
Glomus.Torrecillas12b.Glo.G13_VTX00409 | 3 | 19,765 | |
Glomus.sp._VTX00304 | 1 | 16,515 | |
Glomus.Wirsel.OTU16_VTX00156 | 1 | 16,510 | |
Glomus.Glo-C_VTX00323 | 2 | 10,763 | |
Glomus.sp._VTX00412 | 1 | 8217 | |
Glomus.Yamato09.A1_VTX00092 | 2 | 6499 | |
Glomus.Glo8_VTX00175 | 1 | 3781 | |
Glomus.MO-G5_VTX00219 | 1 | 3736 | |
Glomus.MO-G8_VTX00130 | 1 | 3311 | |
Glomus_sp._Glo56 | 1 | 3092 | |
Glomus.MO-G44_VTX00410 | 2 | 3038 | |
Glomus.Alguacil09b.Glo.G3_VTX00389 | 1 | 2478 | |
Glomus.MO-G23_VTX00222 | 1 | 2337 | |
Glomus.sp._VTX00301 | 2 | 2292 | |
Glomus.PF9_VTX00154 | 1 | 1567 | |
Glomus_sp._C/3-10 | 2 | 1305 | |
Glomus.ORVIN.GLO3E_VTX00309 | 1 | 1290 | |
Glomus.MO-G20_VTX00143 | 1 | 1279 | |
Glomus.Alguacil09b.Glo.G3_VTX00312 | 1 | 802 | |
Glomus.NF05_VTX00322 | 1 | 657 | |
Glomus.sp._VTX00420 | 1 | 548 | |
Glomus.MO-G22_VTX00125 | 1 | 404 | |
Glomus.Alguacil12a.Glo.G7_VTX00363 | 1 | 300 | |
Glomus.NF23_VTX00326 | 2 | 260 | |
Glomus.Wirsel.OTU13_VTX00140 | 1 | 159 | |
Glomus.Glo2_VTX00280 | 1 | 96 | |
Glomus.Wirsel.OTU6_VTX00202 | 1 | 75 | |
Glomus.ORVIN.GLO3D_VTX00310 | 1 | 68 | |
Glomus.Wirsel.OTU14_VTX00137 | 2 | 17 | |
Glomus.mosseae_VTX00067 | 1 | 9 | |
Glomus.MO-G16_VTX00072 | 1 | 6 | |
Glomus.sp._VTX00365 | 1 | 5 | |
Glomus.Whitfield.type.17_VTX00195 | 1 | 5 | |
Claroideoglomeraceae (3 VT, 88 reads, 0.02%) | Claroideoglomus.Torrecillas12b.Glo.G5_VTX00402 | 2 | 37 |
Claroideoglomus.lamellosum_VTX00193 | 1 | 37 | |
Claroideoglomus.ORVIN.GLO4_VTX00278 | 2 | 14 | |
Paraglomeraceae (3 VT, 832 reads, 0.17%) | Paraglomus.Alguacil12a.Para.1_VTX00348 | 12 | 787 |
Paraglomus.laccatum_VTX00281 | 1 | 37 | |
Paraglomus.Alguacil12b.ACA1_VTX00352 | 1 | 8 | |
Gigasporaceae (1 VT, 6 reads, 0.00%) | Gigaspora.decipiens_VTX00039 | 1 | 6 |
Sample | Sobs | Ace | Shannon | Simpson |
---|---|---|---|---|
Citrange/Citrange | 55.00 ± 2.65 ab | 60.74 ± 4.56 ab | 1.79 ± 0.2 b | 0.34 ± 0.07 a |
Orange/Citrange | 48.33 ± 4.26 b | 54.83 ± 3.46 b | 1.80 ± 0.52 b | 0.35 ± 0.16 a |
Poncirus/Poncirus | 55.33 ± 2.91 ab | 59.37 ± 3.93 ab | 2.43 ± 0.18 ab | 0.15 ± 0.04 ab |
Pummelo/Poncirus | 56.33 ± 6.36 ab | 58.56 ± 5.99 ab | 2.27 ± 0.17 ab | 0.16 ± 0.02 ab |
Mandarin/Poncirus | 58.00 ± 3.79 ab | 64.47 ± 5.04 ab | 2.28 ± 0.14 ab | 0.15 ± 0.03 ab |
Orange/Poncirus | 63.00 ± 4.04 a | 77.40 ± 12.21 a | 2.64 ± 0.14 a | 0.11 ± 0.02 b |
AMF Species | Citrange/ Citrange | Orange/ Citrange | Poncirus/ Poncirus | Pummelo/ Poncirus | Mandarin/ Poncirus | Orange/ Poncirus |
---|---|---|---|---|---|---|
Glomus.MO-G44_VTX00410 | 0 ± 0 b | 0 ± 0 b | 0.05 ± 0.05 b | 0.38 ± 0.38 b | 0.26 ± 0.18 b | 2.27 ± 1.29 a |
Glomus.MO-G8_VTX00130 | 0.01 ± 0 b | 0 ± 0 b | 0.07 ± 0.05 b | 0.38 ± 0.2 ab | 0.47 ± 0.27 ab | 2.29 ± 1.65 a |
Glomus.sp._VTX00301 | 0.03 ± 0.01 b | 0.01 ± 0.01 b | 0.1 ± 0.02 b | 0.74 ± 0.26 ab | 1.33 ± 0.61 a | 0.04 ± 0.02 b |
Glomus.NF05_VTX00322 | 0.03 ± 0.03 b | 0 ± 0 b | 0.04 ± 0.03 b | 0.08 ± 0.08 b | 0 ± 0 b | 0.49 ± 0.25 a |
Paraglomus.Alguacil12a.Para.1_ VTX00348 | 0.04 ± 0.01 b | 0.06 ± 0.01 b | 0.05 ± 0.02 b | 0.1 ± 0.07 b | 0.4 ± 0.23 a | 0.13 ± 0.04 ab |
Glomus.sp._VTX00304 | 0.18 ± 0.06 b | 0.08 ± 0.08 b | 0.63 ± 0.13 ab | 6.71 ± 0.48 a | 8.05 ± 4.37 a | 0.51 ± 0.29 ab |
Glomus.Wirsel.OTU16_VTX00156 | 0.21 ± 0.09 b | 0.08 ± 0.08 b | 0.65 ± 0.16 b | 5.4 ± 1.62 ab | 9.29 ± 4.52 a | 0.5 ± 0.26 b |
Glomus.ORVIN.GLO3E_VTX00309 | 0.86 ± 0.33 a | 0 ± 0 b | 0 ± 0 b | 0 ± 0 b | 0 ± 0 b | 0.37 ± 0.37 ab |
Glomus.Torrecillas12b.Glo.G13_ VTX00409 | 0.91 ± 0.33 b | 0.7 ± 0.26 b | 0.64 ± 0.35 b | 8.15 ± 5.2 a | 5.83 ± 0.66 ab | 3.08 ± 0.14 ab |
Glomus.NF13_VTX00419 | 1.55 ± 1.29 ab | 6.74 ± 3.42 ab | 2.47 ± 0.3 ab | 2.19 ± 0.79 ab | 0.83 ± 0.2 b | 7.79 ± 4 a |
Glomus.Glo-C_VTX00323 | 1.59 ± 0.59 b | 0.74 ± 0.40 b | 8.03 ± 3.78 a | 0.19 ± 0.18 b | 0.02 ± 0.01 b | 0.22 ± 0.19 b |
Glomus.Glo7_VTX00214 | 3.04 ± 1.14 b | 3.65 ± 2.81 b | 15.18 ± 7.15 a | 2.68 ± 2.23 b | 0.73 ± 0.59 b | 5.34 ± 3.88 ab |
Glomus.Yamato09.A2_VTX00248 | 3.52 ± 2.41 b | 2.75 ± 1.38 b | 20.17 ± 1.71 a | 11.79 ± 6.66 a | 7.13 ± 5.3 ab | 20.2 ± 5.78 a |
Glomus.acnaGlo2_VTX00155 | 3.58 ± 0.63 b | 6.61 ± 4.42 b | 6.71 ± 1.49 b | 27.42 ± 4.17 a | 12.83 ± 5.49 b | 12.64 ± 3.68 b |
Glomus.Yamato08.A1_VTX00100 | 54.49 ± 8.96 a | 50.67 ± 17.75 a | 19.04 ± 10.93 b | 0.22 ± 0.17 b | 0.05 ± 0.01 b | 5.42 ± 4.54 b |
Sample Name | Scion | Scion Genotype | Rootstock | Rootstock Genotype |
---|---|---|---|---|
Poncirus/Poncirus | Poncirus | Poncirus trifoliata | Poncirus | Poncirus trifoliata |
Citrange/Citrange | Citrange | Citrus sinensis×Poncirus trifoliata | Citrange | Citrus sinensis×Poncirus trifoliata |
Mandarin/Poncirus | Mandarin | Citrus reticulate | Poncirus | Poncirus trifoliata |
Pummelo/Poncirus | Pummelo | Citrus grandis | Poncirus | Poncirus trifoliata |
Orange/Poncirus | Newhall sweet orange | Citrus sinensis | Poncirus | Poncirus trifoliata |
Orange/Citrange | Newhall sweet orange | Citrus sinensis | Citrange | Citrus sinensis×Poncirus trifoliata |
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Song, F.; Bai, F.; Wang, J.; Wu, L.; Jiang, Y.; Pan, Z. Influence of Citrus Scion/Rootstock Genotypes on Arbuscular Mycorrhizal Community Composition under Controlled Environment Condition. Plants 2020, 9, 901. https://doi.org/10.3390/plants9070901
Song F, Bai F, Wang J, Wu L, Jiang Y, Pan Z. Influence of Citrus Scion/Rootstock Genotypes on Arbuscular Mycorrhizal Community Composition under Controlled Environment Condition. Plants. 2020; 9(7):901. https://doi.org/10.3390/plants9070901
Chicago/Turabian StyleSong, Fang, Fuxi Bai, Juanjuan Wang, Liming Wu, Yingchun Jiang, and Zhiyong Pan. 2020. "Influence of Citrus Scion/Rootstock Genotypes on Arbuscular Mycorrhizal Community Composition under Controlled Environment Condition" Plants 9, no. 7: 901. https://doi.org/10.3390/plants9070901