Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit
Abstract
:1. Introduction
2. Results
2.1. Transcriptomic Analysis of As1, As2, and WT in Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana
2.2. Expression Profiles of Selected Relevant Genes in Starch and Sucrose Metabolism
2.3. Expression Profiles of Selected Relevant Genes in Glycolytic Metabolism
2.4. Gene Expressions of Sucrose Synthase, Sucrose Phosphate Synthase, and Neutral Invertase as Analyzed by Avadis and Trinity
2.5. Expression Profiles of the Sucrose Biosynthesis Pathway Genes Based on Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Data
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Total RNA Extraction and Whole-Transcriptome Deep Sequencing
4.3. Real-Time Quantitative RT-PCR
4.4. R Programming for Heat Map Illustration
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Xia, Y.; Chiu, C.-H.; Do, Y.-Y.; Huang, P.-L. Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit. Plants 2020, 9, 1120. https://doi.org/10.3390/plants9091120
Xia Y, Chiu C-H, Do Y-Y, Huang P-L. Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit. Plants. 2020; 9(9):1120. https://doi.org/10.3390/plants9091120
Chicago/Turabian StyleXia, Yan, Chien-Hsiang Chiu, Yi-Yin Do, and Pung-Ling Huang. 2020. "Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit" Plants 9, no. 9: 1120. https://doi.org/10.3390/plants9091120