Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Camellia sinensis L. O. Kuntze) and Characterization of Amino Acid Transporter CsCAT9.1
Abstract
:1. Introduction
2. Results
2.1. Transcriptome Analysis Overview Based on PacBio RSII and NGS
2.2. Unigenes Annotation
2.3. Global Analysis of Differentially Expressed Genes (DEGs)
2.4. Transcript Analysis of Genes Involved in N Uptake and Transport
2.5. Transcript Analysis for Genes Involved in N Assimilation
2.6. RNA Sequencing Validation by qRT-PCR
2.7. Subcellular Localization for a Putative Amino Acid Transporter of CsCAT9.1 with Differential Broad-Spectrum for Substrate Selectivity
3. Discussion
4. Materials and Methods
4.1. Tea Plant Materials and N Treatments
4.2. RNA Extraction, Next Generation Sequencing, SMRT Sequencing and Differential Expression Analysis
4.3. Verification of RNA-seq Data by qRT-PCR
4.4. Functional Annotation for Transcriptome Unigenes
4.5. CsCAT9.1 Cloning and Transformation in Arabidopsis
4.6. Subcellular Localization and Amino Acid Substrates Identification of CsCAT9.1
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Databases | Number of Unigenes | Percentage |
---|---|---|
NR | 41,238 | 96.07% |
SwissProt | 35,362 | 82.38% |
KEGG | 18,264 | 42.55% |
GO | 22,983 | 53.54% |
KOG | 26,841 | 62.53% |
Annotated in all databases | 10,615 | 24.73% |
Annotated in at least one database | 41,325 | 96.28% |
Total unigenes | 42,923 | 100% |
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Zhang, X.; Liu, H.; Pilon-Smits, E.; Huang, W.; Wang, P.; Wang, M.; Guo, F.; Wang, Y.; Li, R.; Zhao, H.; et al. Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Camellia sinensis L. O. Kuntze) and Characterization of Amino Acid Transporter CsCAT9.1. Plants 2020, 9, 1218. https://doi.org/10.3390/plants9091218
Zhang X, Liu H, Pilon-Smits E, Huang W, Wang P, Wang M, Guo F, Wang Y, Li R, Zhao H, et al. Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Camellia sinensis L. O. Kuntze) and Characterization of Amino Acid Transporter CsCAT9.1. Plants. 2020; 9(9):1218. https://doi.org/10.3390/plants9091218
Chicago/Turabian StyleZhang, Xinwan, Hongling Liu, Elizabeth Pilon-Smits, Wei Huang, Pu Wang, Mingle Wang, Fei Guo, Yu Wang, Ruiyuan Li, Hua Zhao, and et al. 2020. "Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Camellia sinensis L. O. Kuntze) and Characterization of Amino Acid Transporter CsCAT9.1" Plants 9, no. 9: 1218. https://doi.org/10.3390/plants9091218