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Chemosensors
Volume 10
Issue 4
10.3390/chemosensors10040149
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Article

Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires

by
Martyna Jankowska
1,
Karolina Sulowska
1,
Kamil Wiwatowski
1,
Joanna Niedziółka-Jönsson
2 and
Sebastian Mackowski
1,*
1
Nanophotonics Group, Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Torun, Grudziadzka 5, 87-100 Torun, Poland
2
Institute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Chemosensors 2022, 10(4), 149; https://doi.org/10.3390/chemosensors10040149
Submission received: 31 January 2022 / Revised: 30 March 2022 / Accepted: 12 April 2022 / Published: 18 April 2022
(This article belongs to the Special Issue Nanomaterials-Based Sensors)
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Abstract

In this work, we aimed to apply fluorescence microscopy to image protein conjugation to Ni-NTA modified silver nanowires in real time via the His-tag attachment. First, a set of experiments was designed and performed for the mixtures of proteins and silver nanowires in order to demonstrate plasmon enhancement of mCherry protein fluorescence as well as the ability to image fluorescence of single molecules. The results indicated strong enhancement of single-protein fluorescence emission upon coupling with silver nanowires. This conclusion was supported by a decrease in the fluorescence decay time of mCherry proteins. Real-time imaging was carried out for a structure created by dropping protein solution onto a glass substrate with functionalized silver nanowires. We observed specific attachment of mCherry proteins to the nanowires, with the recognition time being much longer than in the case of streptavidin–biotin conjugation. This result indicated that it is possible to design a universal and efficient real-time sensing platform with plasmonically active functionalized silver nanowires.
Keywords: silver nanowires; surface functionalizatio; conjugation; fluorescence imaging; photoactive protein; biosensing silver nanowires; surface functionalizatio; conjugation; fluorescence imaging; photoactive protein; biosensing

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MDPI and ACS Style

Jankowska, M.; Sulowska, K.; Wiwatowski, K.; Niedziółka-Jönsson, J.; Mackowski, S. Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires. Chemosensors 2022, 10, 149. https://doi.org/10.3390/chemosensors10040149

AMA Style

Jankowska M, Sulowska K, Wiwatowski K, Niedziółka-Jönsson J, Mackowski S. Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires. Chemosensors. 2022; 10(4):149. https://doi.org/10.3390/chemosensors10040149

Chicago/Turabian Style

Jankowska, Martyna, Karolina Sulowska, Kamil Wiwatowski, Joanna Niedziółka-Jönsson, and Sebastian Mackowski. 2022. "Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires" Chemosensors 10, no. 4: 149. https://doi.org/10.3390/chemosensors10040149

APA Style

Jankowska, M., Sulowska, K., Wiwatowski, K., Niedziółka-Jönsson, J., & Mackowski, S. (2022). Real-Time Fluorescence Imaging of His-Tag-Driven Conjugation of mCherry Proteins to Silver Nanowires. Chemosensors, 10(4), 149. https://doi.org/10.3390/chemosensors10040149

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