Next Article in Journal
Fabrication of Doxorubicin-Loaded Lipid-Based Nanocarriers by Microfluidic Rapid Mixing
Next Article in Special Issue
Autophagy: Guardian of Skin Barrier
Previous Article in Journal
Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation
Previous Article in Special Issue
ER-phagy in the Occurrence and Development of Cancer
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomedicines
Volume 10
Issue 6
10.3390/biomedicines10061257
Review Report
biomedicines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Methamphetamine Dysregulates Macrophage Functions and Autophagy to Mediate HIV Neuropathogenesis

Biomedicines 2022, 10(6), 1257; https://doi.org/10.3390/biomedicines10061257
by John M. Barbaro 1, Simone Sidoli 2, Ana Maria Cuervo 3 and Joan W. Berman 1,4,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Biomedicines 2022, 10(6), 1257; https://doi.org/10.3390/biomedicines10061257
Submission received: 29 March 2022 / Revised: 19 May 2022 / Accepted: 24 May 2022 / Published: 27 May 2022
(This article belongs to the Special Issue Autophagy in Inflammation-Associated Damage and Diseases)

Round 1

Reviewer 1 Report

This is a very well conducted study. However, I feel that introduction and discussion section are too heavy and should be shortened. Furthermore the discussion ends abruptly; it requires a few closing and concluding sentences.

Author Response

Please see the attached file.

Author Response File: Author Response.pdf

Reviewer 2 Report

The study is well-conceived and designed, well written and described; methods are properly chosen and rigorous. However, I have a main concern regarding this study, related to the role of HIV in the proteomic and functional changes described. The authors compared infected macrophages treated with meth with HIV-infected, untreated cells. However, there are no uninfected, control cells that can help to understand if the proteome effects attributed to Meth are present only in an HIV background, or if they are independent of HIV. I suggest adding an HIV- control or, if this is not possible, comparing HIV+ data with data reported in the literature and/or present in a public proteomic database.  This point should have at least been discussed.

 

Concerning methods:

  1. It is not clear how the authors differentiate monocytes into macrophages.
  2. Since the authors have focused their attention on mitochondria, and they found significant changes in the expression of OXPHOS related proteins, I think it could be more informative to distinguish ROS produced by mitochondria (e.g. superoxide anion, by using MitoSOX) from total ROS (which can include ROS produced by phagocyte oxidase). If it is not possible to perform this experiment, I suggest adding this point in the discussion, as a limitation of the study.

 

Concerning results:

Can the observations made by the authors be dependent on the phenotype/differentiation status of macrophages? Have the authors checked the typical markers of M1/M2  macrophages (being aware of the limitation of M1/M2 dichotomy)?

 

 

 

Author Response

Please see the attached file.

Author Response File: Author Response.pdf

Back to TopTop