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Volume 12
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10.3390/biomedicines12102368
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Article

Mitochondrial Creatine Kinase 2 (Ckmt2) as a Plasma-Based Biomarker for Evaluating Reperfusion Injury in Acute Myocardial Infarction

by
Alexander Lang
1,
Daniel Oehler
1,
Marcel Benkhoff
1,
Yvonne Reinders
2,
Maike Barcik
1,
Khatereh Shahrjerdi
1,
Madlen Kaldirim
1,
Albert Sickmann
2,3,4,
Lisa Dannenberg
1,
Amin Polzin
1,5,
Susanne Pfeiler
1,
Malte Kelm
1,5,
Maria Grandoch
5,6,
Christian Jung
1,5 and
Norbert Gerdes
1,5,*
1
Division of Cardiology, Pulmonology and Vascular Medicine, Medical Faculty and University Hospital, Heinrich-Heine University, 40225 Düsseldorf, Germany
2
Leibniz-Institut für Analytische Wissenschaften—ISAS—e.V., 44139 Dortmund, Germany
3
Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany
4
Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen AB24 3FX, UK
5
Cardiovascular Research Institute Düsseldorf (CARID), Medical Faculty and University Hospital, Heinrich-Heine University, 40225 Düsseldorf, Germany
6
Institute for Translational Pharmacology, Medical Faculty and University Hospital, Heinrich-Heine University, 40225 Düsseldorf, Germany
*
Author to whom correspondence should be addressed.
Biomedicines 2024, 12(10), 2368; https://doi.org/10.3390/biomedicines12102368
Submission received: 6 September 2024 / Revised: 23 September 2024 / Accepted: 15 October 2024 / Published: 16 October 2024
(This article belongs to the Special Issue Molecular Mechanisms and Translational Aspects of Ischemia-Reperfusion Injury)
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Abstract

Background/Objectives: Acute myocardial infarction (AMI), characterized by irreversible heart muscle damage and impaired cardiac function caused by myocardial ischemia, is a leading cause of global mortality. The damage associated with reperfusion, particularly mitochondrial dysfunction and reactive oxygen species (ROS) formation, has emerged as a crucial factor in the pathogenesis of cardiac diseases, leading to the recognition of mitochondrial proteins as potential markers for myocardial damage. This study aimed to identify differentially expressed proteins based on the type of cardiac injury, in particular those with and without reperfusion. Methods: Male C57Bl/6J mice were either left untreated, sham-operated, received non-reperfused AMI, or reperfused AMI. Twenty-four hours after the procedures, left ventricular (LV) function and morphological changes including infarct size were determined using echocardiography and triphenyl tetrazolium chloride (TTC) staining, respectively. In addition, plasma was isolated and subjected to untargeted mass spectrometry and, further on, the ELISA-based validation of candidate proteins. Results: We identified mitochondrial creatine kinase 2 (Ckmt2) as a differentially regulated protein in plasma of mice with reperfused but not non-reperfused AMI. Elevated levels of Ckmt2 were significantly associated with infarct size and impaired LV function following reperfused AMI, suggesting a specific involvement in reperfusion damage. Conclusions: Our study highlights the potential of plasma Ckmt2 as a biomarker for assessing reperfusion injury and its impact on cardiac function and morphology in the acute phase of MI.
Keywords: myocardial infarction; reperfusion injury; mitochondrial damage; biomarker myocardial infarction; reperfusion injury; mitochondrial damage; biomarker

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MDPI and ACS Style

Lang, A.; Oehler, D.; Benkhoff, M.; Reinders, Y.; Barcik, M.; Shahrjerdi, K.; Kaldirim, M.; Sickmann, A.; Dannenberg, L.; Polzin, A.; et al. Mitochondrial Creatine Kinase 2 (Ckmt2) as a Plasma-Based Biomarker for Evaluating Reperfusion Injury in Acute Myocardial Infarction. Biomedicines 2024, 12, 2368. https://doi.org/10.3390/biomedicines12102368

AMA Style

Lang A, Oehler D, Benkhoff M, Reinders Y, Barcik M, Shahrjerdi K, Kaldirim M, Sickmann A, Dannenberg L, Polzin A, et al. Mitochondrial Creatine Kinase 2 (Ckmt2) as a Plasma-Based Biomarker for Evaluating Reperfusion Injury in Acute Myocardial Infarction. Biomedicines. 2024; 12(10):2368. https://doi.org/10.3390/biomedicines12102368

Chicago/Turabian Style

Lang, Alexander, Daniel Oehler, Marcel Benkhoff, Yvonne Reinders, Maike Barcik, Khatereh Shahrjerdi, Madlen Kaldirim, Albert Sickmann, Lisa Dannenberg, Amin Polzin, and et al. 2024. "Mitochondrial Creatine Kinase 2 (Ckmt2) as a Plasma-Based Biomarker for Evaluating Reperfusion Injury in Acute Myocardial Infarction" Biomedicines 12, no. 10: 2368. https://doi.org/10.3390/biomedicines12102368

APA Style

Lang, A., Oehler, D., Benkhoff, M., Reinders, Y., Barcik, M., Shahrjerdi, K., Kaldirim, M., Sickmann, A., Dannenberg, L., Polzin, A., Pfeiler, S., Kelm, M., Grandoch, M., Jung, C., & Gerdes, N. (2024). Mitochondrial Creatine Kinase 2 (Ckmt2) as a Plasma-Based Biomarker for Evaluating Reperfusion Injury in Acute Myocardial Infarction. Biomedicines, 12(10), 2368. https://doi.org/10.3390/biomedicines12102368

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