Immune Responses to Mycobacterium tuberculosis Infection in the Liver of Diabetic Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria and Chemicals
2.2. Aerosol Infection of Mice, Treatment, and Bacterial CFU Assay
2.3. Histology Staining of Liver Sections and Morphometry
2.4. Quantification of Glutathione Levels
2.5. Cytokine Measurement
2.6. Statistical Analysis
3. Results
3.1. Levels of Cytokines and GSH in Liver Lysates at 4 Weeks Post-M. tb Infection and Antibiotics with or without GSH Treatment
3.2. Levels of Cytokines and GSH in the Liver Lysates at 8 Weeks Post-Infection and Treatment
3.3. Effect of GSH with or without RIF on the Liver Pathology of M. tb-Infected db/db Mice
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Badaoui, A.; Sasaninia, K.; Mohan, A.S.; Beever, A.; Kachour, N.; Raien, A.; Kolloli, A.; Kumar, R.; Ramasamy, S.; Subbian, S.; et al. Immune Responses to Mycobacterium tuberculosis Infection in the Liver of Diabetic Mice. Biomedicines 2024, 12, 1370. https://doi.org/10.3390/biomedicines12061370
Badaoui A, Sasaninia K, Mohan AS, Beever A, Kachour N, Raien A, Kolloli A, Kumar R, Ramasamy S, Subbian S, et al. Immune Responses to Mycobacterium tuberculosis Infection in the Liver of Diabetic Mice. Biomedicines. 2024; 12(6):1370. https://doi.org/10.3390/biomedicines12061370
Chicago/Turabian StyleBadaoui, Ali, Kayvan Sasaninia, Aishvaryaa Shree Mohan, Abrianna Beever, Nala Kachour, Anmol Raien, Afsal Kolloli, Ranjeet Kumar, Santhamani Ramasamy, Selvakumar Subbian, and et al. 2024. "Immune Responses to Mycobacterium tuberculosis Infection in the Liver of Diabetic Mice" Biomedicines 12, no. 6: 1370. https://doi.org/10.3390/biomedicines12061370