Effect of Addition of Zero-Valent Iron (Fe) and Magnetite (Fe3O4) on Methane Yield and Microbial Consortium in Anaerobic Digestion of Food Wastewater
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methane Production Potential
2.3. Analysis of Microbial Consortium
2.3.1. DNA Extraction and Quantification
2.3.2. Library Construction and Sequencing
2.4. Chemical Analysis
2.5. Statistical Analysis
3. Results and Discussion
3.1. Methane Yield and Reaction Kinetics
3.2. Microbial Community
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Food Wastewater | |
---|---|---|
pH (-) | 3.53 | |
TS 1 (mg/L) | 102,422 | |
VS 2 (mg/L) | 89,756 | |
TKN 3 (mg/L) | 3870 | |
NH4+-N 4 (mg/L) | 381 | |
CODCr 5 (mg/L) | 166,333 | |
SCODCr 6 (mg/L) | 5474 | |
TVFAs 7 (mg/L as acetate) | 7657 | |
Elemental composition (wt.%, d.b. 8) | C | 46.70 |
H | 6.53 | |
O | 3.27 | |
N | 34.60 | |
S | 0.00 |
Parameters | pH (-) | TS 1 | VS 2 | TKN 3 | NH4+-N 4 | CODCr 5 | SCODCr 6 | Alkalinity (mg L−1 as CaCO3) | TVFAs 7 (mg L−1 as Acetate) |
---|---|---|---|---|---|---|---|---|---|
(mg L−1) | |||||||||
Inoculum | 8.36 | 20,767 | 9511 | 3467 | 2746 | 6017 | 4930 | 12,975 | 41 |
Parameters | Control | Zero-Valent Iron Concentration (%) | |||
---|---|---|---|---|---|
0.25 | 0.50 | 1.00 | 1.50 | ||
Bu 1 (Nm3 kg−1-VSadded) | 0.380 c | 0.418 b | 0.423 ab | 0.425 ab | 0.434 a |
Rm 2 (mL day−1) | 15.73 c | 19.09 b | 19.53 ab | 20.32 a | 19.63 ab |
λ 3 (days) | 0.541 a | 0.352 b | 0.395 b | 0.364 b | 0.065 c |
Bth 4 (Nm3 kg−1-VSadded) | 0.437 | 0.437 | 0.437 | 0.437 | 0.437 |
VSr 5 (%) | 86.95 | 95.72 | 96.73 | 97.28 | 99.45 |
Parameters | Control | Magnetite Concentration (%) | |||
---|---|---|---|---|---|
0.25 | 0.50 | 1.00 | 1.50 | ||
Bu 1 (Nm3 kg−1-VSadded) | 0.380 c | 0.411 b | 0.412 b | 0.431 a | 0.419 b |
Rm 2 (mL day−1) | 15.73 b | 15.94 b | 16.48 b | 18.44 a | 18.26 a |
λ 3 (days) | 0.541 ab | 0.469 b | 0.600 ab | 0.673 a | 0.650 a |
Bth 4 (Nm3 kg−1-VSadded) | 0.437 | 0.437 | 0.437 | 0.437 | 0.437 |
VSr 5 (%) | 86.95 | 94.05 | 94.28 | 98.65 | 95.95 |
Treatments | Solubilized Fe | ||
---|---|---|---|
Soluble 1 Forms | Total 2 Forms | ||
ZVI concentration (%) | 0.25 | 27.0 (0.1) 3 | 530.4 (3.3) |
0.50 | 38.0 (0.3) | 690.1 (1.4) | |
1.00 | 30.1 (0.0) | 606.3 (1.3) | |
1.50 | 34.3 (0.0) | 703.6 (1.1) | |
Magnetite concentration (%) | 0.25 | 24.9 (0.1) | 175.0 (0.1) |
0.50 | 23.5 (0.2) | 182.0 (0.8) | |
1.00 | 24.9 (0.1) | 254.6 (0.8) | |
1.50 | 25.3 (0.0) | 214.9 (0.9) |
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Lee, J.-H.; Lee, J.-H.; Kim, S.-Y.; Yoon, Y.-M. Effect of Addition of Zero-Valent Iron (Fe) and Magnetite (Fe3O4) on Methane Yield and Microbial Consortium in Anaerobic Digestion of Food Wastewater. Processes 2023, 11, 759. https://doi.org/10.3390/pr11030759
Lee J-H, Lee J-H, Kim S-Y, Yoon Y-M. Effect of Addition of Zero-Valent Iron (Fe) and Magnetite (Fe3O4) on Methane Yield and Microbial Consortium in Anaerobic Digestion of Food Wastewater. Processes. 2023; 11(3):759. https://doi.org/10.3390/pr11030759
Chicago/Turabian StyleLee, Jun-Hyeong, Jae-Hyuk Lee, Sang-Yoon Kim, and Young-Man Yoon. 2023. "Effect of Addition of Zero-Valent Iron (Fe) and Magnetite (Fe3O4) on Methane Yield and Microbial Consortium in Anaerobic Digestion of Food Wastewater" Processes 11, no. 3: 759. https://doi.org/10.3390/pr11030759