Glycosyltransferases Expression Changes in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Grown on Different Carbon Sources
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Aerobic Culture Conditions
2.3. Anaerobic Culture Conditions
2.4. Biomass and pH Determination
2.5. Protein Determination
2.6. GTFs Activity Determination
2.7. Zymograms
2.8. RNA-seq Analysis
2.9. Differential Gene Expression Analysis
2.10. Operon Search
2.11. Motif Search
2.12. RT-qPCR
3. Results and Discussion
3.1. Specific Total Activity Decreases in CS Different from Sucrose
3.2. Differential Expression Analysis (DEA): Using Xylose Produces Major Changes in Gene Expression
3.3. Exploring Possible Regulation Pathways of Leuconostoc mesenteroides ATCC 8293 GTFs
3.4. Genomic Context of GTFs Genes
3.5. The Transcription Factor (TF) CcpA
3.6. The Transcription Factor PerR
3.7. The Transcription Factor LexA
3.8. VicK/VicR Two-Component System
3.9. Transcriptomics Validation by Retro-Transcriptase qPCR
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ID Locus | Gene | LOG2 FC (Xyl/Suc) | Xylose | LOG2FC (Fru/Suc) | Fructose |
---|---|---|---|---|---|
LEUM_1409 | levC | −2.726 | Down-regulated | −1.442 | NCD * |
LEUM_1410 | levX | −2.135 | Down-regulated | −0.506 | NCD * |
LEUM_1411 | levL | −2.813 | Down-regulated | −0.500 | NCD * |
LEUM_0857 | dsxD | −2.059 | Down-regulated | −0.081 | NCD * |
LEUM_1747 | dsrD | −0.616 | NCD * | −0.193 | NCD * |
LEUM_1752 | dsrI | −2.220 | Down-regulated | −0.149 | NCD * |
Condition | Total Activity (U/mg Total Protein) | Biomass (OD600 nm) Reached |
---|---|---|
Aerobic | 1.06 ± 0.1 | 6.5 ± 1.2 |
Anaerobic | 1.04 ± 0.1 | 6.3 ± 0.9 |
Sequence Name | Strand | Start | End | p-Value | q-Value | Matched Sequence |
---|---|---|---|---|---|---|
Upstream levC-scrB | negative | 90 | 106 | 2.81 × 10−5 | 0.118 | tgtttcctctctgttaa |
Upstream levLX | positive | 408 | 424 | 8.97 × 10−5 | 0.138 | tgtaatactattgaaat |
RT-qPCR | RNA-Seq | ||
---|---|---|---|
ID | Gene | LOG2FC Xyl/Fru | LOG2FC Xyl/Fru |
LEUM_1409 | levC | −3.73 | −1.67 |
LEUM_1410 | levX | −4.03 | −2.01 |
LEUM_1411 | levL | −3.53 | −2.69 |
LEUM_0857 | dsxD | −6.83 | −2.24 |
LEUM_1747 | dsrD | −1.61 | −0.93 |
LEUM_1752 | dsrI | −4.71 | −2.45 |
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Vallejo-García, L.C.; Sánchez-Olmos, M.d.C.; Gutiérrez-Ríos, R.M.; López Munguía, A. Glycosyltransferases Expression Changes in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Grown on Different Carbon Sources. Foods 2023, 12, 1893. https://doi.org/10.3390/foods12091893
Vallejo-García LC, Sánchez-Olmos MdC, Gutiérrez-Ríos RM, López Munguía A. Glycosyltransferases Expression Changes in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Grown on Different Carbon Sources. Foods. 2023; 12(9):1893. https://doi.org/10.3390/foods12091893
Chicago/Turabian StyleVallejo-García, Luz Cristina, María del Carmen Sánchez-Olmos, Rosa María Gutiérrez-Ríos, and Agustín López Munguía. 2023. "Glycosyltransferases Expression Changes in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Grown on Different Carbon Sources" Foods 12, no. 9: 1893. https://doi.org/10.3390/foods12091893