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Article

Uncovering the Hidden Potential of Phytoene Production by the Fungus Blakeslea trispora

by
Fani Th Mantzouridou
*,
Elpida Sferopoulou
and
Panagiota Thanou
Laboratory of Food Chemistry and Technology, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Foods 2024, 13(18), 2882; https://doi.org/10.3390/foods13182882
Submission received: 27 July 2024 / Revised: 6 September 2024 / Accepted: 8 September 2024 / Published: 11 September 2024
(This article belongs to the Topic Microbes and Their Products for Sustainable Human Life)

Abstract

Phytoene is an uncommon linear carotene within the carotenoid group as it is colorless due to its short chromophore. Recent research constitutes a relatively new area which has emerged from phytoene’s importance as a major dietary carotenoid promoting health and appearance. Its resources point to the potential of biotechnological production systems. Our work has been designed to study the efficacy of two colored carotenoid biosynthesis inhibitors, diphenylamine and 2-methyl imidazole, and one sterol biosynthesis inhibitor, terbinafine, to modify the metabolic flux in mated cultures of Blakeslea trispora to achieve maximum phytoene production. Bioprocess kinetics optimized by response surface methodology and monitored by high-performance liquid chromatography revealed maximum phytoene content (5.02 mg/g dry biomass) and yield (203.91 mg/L culture medium) comparable or even higher than those reported for other potent phytoene microbial producers. The in vivo antioxidant activity of phytoene-rich carotenoid extract from fungal cells was also considered and discussed.
Keywords: phytoene; Blakeslea trispora; diphenylamine; 2-methyl imidazole; terbinafine; β-carotene; ergosterol; in vivo antioxidant activity phytoene; Blakeslea trispora; diphenylamine; 2-methyl imidazole; terbinafine; β-carotene; ergosterol; in vivo antioxidant activity

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MDPI and ACS Style

Mantzouridou, F.T.; Sferopoulou, E.; Thanou, P. Uncovering the Hidden Potential of Phytoene Production by the Fungus Blakeslea trispora. Foods 2024, 13, 2882. https://doi.org/10.3390/foods13182882

AMA Style

Mantzouridou FT, Sferopoulou E, Thanou P. Uncovering the Hidden Potential of Phytoene Production by the Fungus Blakeslea trispora. Foods. 2024; 13(18):2882. https://doi.org/10.3390/foods13182882

Chicago/Turabian Style

Mantzouridou, Fani Th, Elpida Sferopoulou, and Panagiota Thanou. 2024. "Uncovering the Hidden Potential of Phytoene Production by the Fungus Blakeslea trispora" Foods 13, no. 18: 2882. https://doi.org/10.3390/foods13182882

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