The Integrated Analysis of miRNome and Degradome Sequencing Reveals the Regulatory Mechanisms of Seed Development and Oil Biosynthesis in Pecan (Carya illinoinensis)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Sample Collection
2.2. An Analysis of the Oil Content and Components and the Soluble Sugar Content
2.3. Small RNA Library Construction and Sequencing
2.4. Analysis of Small RNA Sequences
2.5. RNA Isolation and Transcriptome Sequencing
2.6. Differentially Expressed miRNAs and Target Prediction
2.7. Validation of the Expression Patterns of miRNAs and Target Genes
2.8. Statistical Analysis
3. Results
3.1. Oil and Sugar Contents and Fatty Acid Composition Analyses
3.2. Sequence Analysis of Small RNAs
3.3. Identification of Known and Novel MicroRNAs in Developing Pecan Seeds
3.4. Differentially Expressed miRNAs during Pecan Seed Development
3.5. Expression Trends of DEMs during Seed Development and Oil Biosynthesis in Pecan
3.6. Identification and Characterization of miRNA Targets
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | Known miRNAs | Novel miRNAs | Total |
---|---|---|---|
DAF120 | 152 | 317 | 469 |
DAF135 | 189 | 320 | 509 |
DAF150 | 185 | 314 | 499 |
Total | 365 | 321 | 686 |
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Zhu, K.; Wei, L.; Ma, W.; Zhao, J.; Chen, M.; Wei, G.; Liu, H.; Tan, P.; Peng, F. The Integrated Analysis of miRNome and Degradome Sequencing Reveals the Regulatory Mechanisms of Seed Development and Oil Biosynthesis in Pecan (Carya illinoinensis). Foods 2024, 13, 2934. https://doi.org/10.3390/foods13182934
Zhu K, Wei L, Ma W, Zhao J, Chen M, Wei G, Liu H, Tan P, Peng F. The Integrated Analysis of miRNome and Degradome Sequencing Reveals the Regulatory Mechanisms of Seed Development and Oil Biosynthesis in Pecan (Carya illinoinensis). Foods. 2024; 13(18):2934. https://doi.org/10.3390/foods13182934
Chicago/Turabian StyleZhu, Kaikai, Lu Wei, Wenjuan Ma, Juan Zhao, Mengyun Chen, Guo Wei, Hui Liu, Pengpeng Tan, and Fangren Peng. 2024. "The Integrated Analysis of miRNome and Degradome Sequencing Reveals the Regulatory Mechanisms of Seed Development and Oil Biosynthesis in Pecan (Carya illinoinensis)" Foods 13, no. 18: 2934. https://doi.org/10.3390/foods13182934