Exploring the Brine Microbiota of a Traditional Norwegian Fermented Fish Product (Rakfisk) from Six Different Producers during Two Consecutive Seasonal Productions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production Conditions
2.2. Experimental Design and Sampling
2.3. Chemical Analysis
2.4. Culture-Dependent Microbial Characterization
2.5. Culture-Independent Microbial Characterization
2.5.1. DNA Isolation from Rakfisk Brine
2.5.2. Microbiota Analyses Using a Direct Dideoxy Sequencing Method
2.5.3. MANOVA
2.5.4. Microbiota Analysis Using Illumina Sequencing
2.5.5. Total Number of Bacteria Using Quantitative Real-Time PCR
2.6. 16S rDNA Sequencing of LAB Isolated from MRS Agar
3. Results
3.1. Chemical Measurements
3.2. Plate Counts and Total Number of Bacteria Using qPCR
3.3. Microbiota Analysis Using Culture-Independent Characterization
3.3.1. Direct Dideoxy Sequencing
3.3.2. Illumina (MiSeq) Sequencing
3.4. Species Determination of LAB Isolated from MRS Agar
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgements
Conflicts of Interest
References
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Producer ID | Fish Species/Origin b | Temperature °C | NaCl % (w/v) d | Sugar Addition | Starter Culture Addition g | Salting Method i | Sampling k |
---|---|---|---|---|---|---|---|
1 a | Char/A | 3.5/4.5 c | 5.8/5.7 | No | No | Dry | Complete |
2 | Char/B | 5.0 | 3.5/4.0 | Yes e | No h | Brine | Complete |
3 | Trout/C | 7.0 | 4.9/4.2 | No | No | Dry | Complete |
4 | Trout/C | 7.0 | 4.8/5.1 | Yes f | Yes | Dry | Year 2+ |
5 | Trout/D | 7.5 | 4.9/3.9 | No | No | Dry | Year 2+ |
6 | Trout/E | 6.0 | 6.9/7.4 | No | No | Dry | Year 2+ m |
Category | Explained Variance (%) | p-Value |
---|---|---|
Producer a | 45.43 | <0.0001 |
Year b | 6.98 | 0.0001 |
Producer and year c | 16.99 | <0.0001 |
Error | 27.42 |
Producers | Psychrobacter | Lactobacillus | ||||
---|---|---|---|---|---|---|
0–7 d | 63–91 d | p-Value | 0–7 d | 63–91 d | p-Value | |
1 | 30.7 ± 20.6 | 50.5 ± 31.5 | 0.2 | 5.4 ± 2.9 | 5.7 ± 8.6 | 0.9 |
2 | 27.1 ± 18.3 | 0.5 ± 0.9 | 0.02 | 0.1 ± 0.2 | 80.5 ± 9.10 | <0.00001 a |
3 | 2.7 ± 5.3 | 7. 6 ± 6.8 | 0.1 | 16.5 ± 11.1 | 38.3 ± 24.3 | 0.04 a |
4 | 5.9 ± 3.2 | 1.2 ± 1.0 | 0.02 | 12.4 ± 7.5 | 79.6 ± 11.2 | <0.000001 a |
5 | 9.2 ± 18.5 | 25.8 ± 20.7 | 0.2 | 18.7 ± 11.1 | 23.4 ± 34.7 | 0.8 |
6 | 25.5 ± 16.8 | 41.6 ± 14.2 | 0.09 | 10.6 ± 13.6 | 7.7 ± 9.1 | 0.7 |
1 b | ND | 10.0 ± 4.1 | 0.05 | ND | 72.1 ± 17.2 | <0.00001 a,c |
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Bjerke, G.A.; Rudi, K.; Avershina, E.; Moen, B.; Blom, H.; Axelsson, L. Exploring the Brine Microbiota of a Traditional Norwegian Fermented Fish Product (Rakfisk) from Six Different Producers during Two Consecutive Seasonal Productions. Foods 2019, 8, 72. https://doi.org/10.3390/foods8020072
Bjerke GA, Rudi K, Avershina E, Moen B, Blom H, Axelsson L. Exploring the Brine Microbiota of a Traditional Norwegian Fermented Fish Product (Rakfisk) from Six Different Producers during Two Consecutive Seasonal Productions. Foods. 2019; 8(2):72. https://doi.org/10.3390/foods8020072
Chicago/Turabian StyleBjerke, Guro Alette, Knut Rudi, Ekaterina Avershina, Birgitte Moen, Hans Blom, and Lars Axelsson. 2019. "Exploring the Brine Microbiota of a Traditional Norwegian Fermented Fish Product (Rakfisk) from Six Different Producers during Two Consecutive Seasonal Productions" Foods 8, no. 2: 72. https://doi.org/10.3390/foods8020072
APA StyleBjerke, G. A., Rudi, K., Avershina, E., Moen, B., Blom, H., & Axelsson, L. (2019). Exploring the Brine Microbiota of a Traditional Norwegian Fermented Fish Product (Rakfisk) from Six Different Producers during Two Consecutive Seasonal Productions. Foods, 8(2), 72. https://doi.org/10.3390/foods8020072