A Synthetic Bio-Absorbable Membrane in Guided Bone Regeneration in Dehiscence-Type Defects: An Experimental In Vivo Investigation in Dogs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Sample
2.2. Surgical Interventions
- The test group using deproteinized bovine bone mineral (DBBM) (BioOss®, Geistlich Pharma, Wolhusen, Switzerland) combined with a synthetic polylactic membrane (PLAB) (GUIDOR®, Sunstar, Schlieren, Switzerland)
- The positive control group using DBBM (BioOss®, Geistlich Pharma, Wolhusen, Switzerland) combined with a natural porcine collagen membrane (NPCM) (BioGide®, Geistlich Pharma, Wolhusen, Switzerland)
- The negative control group using only the synthetic polylactic membrane (PLAB) (GUIDOR®, Sunstar, Schlieren, Switzerland)
2.3. Histological Analysis
- NBT: Newly formed bone thickness at 1, 2, and 3 mm apical to the implant shoulder, described as the distance from the implant surface and the most buccal bone tissue.
- AGT: Augmented tissue thickness at 1, 2, and 3 mm apical to the implant shoulder (barrier space maintenance capacity), described as the distance from the implant surface to the inner part of the identified barrier membrane.
2.4. Decalcified Histology
2.5. Immunohistochemical Analysis
2.6. Data Analysis
3. Results
3.1. Histological Observations
3.2. Histo-Morphometric Measurements
3.2.1. Newly Formed Bone at 4 Weeks
3.2.2. Newly Formed Tissue Thickness at 4 Weeks
3.2.3. Newly Formed Buccal Bone at 12 Weeks
3.2.4. Newly Formed Tissue Thickness at 12 Weeks
3.3. Bone to Implant Contact
3.4. Immunohistochemical Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Healing Time | 1 mm | 2 mm | 3 mm |
---|---|---|---|---|
Test Group | 4 weeks | 0.39 ± 0.68 | 0.79 ± 1.01 | 0.84 ± 0.93 |
+C Group | 4 weeks | 0.63 ± 0.14 | 0.72 ± 0.16 | 1.30 ± 0.26 |
-C Group | 4 weeks | 0.24 ± 0.21 | 0.45 ± 0.41 | 0.62 ± 0.62 |
Test Group | 12 weeks | 0.47 ± 0.62 | 0.79 ± 1.01 | 100 ± 1.19 |
+C Group | 12 weeks | 0.49 ± 0.64 | 0.79 ± 0.82 | 1.18 ± 0.79 |
-C Group | 12 weeks | 0.27 ± 0.25 | 0.59 ± 0.75 | 0.84 ± 0.60 |
Group | Healing Time | 1 mm | 2 mm | 3 mm |
---|---|---|---|---|
Test Group | 4 weeks | 1.52 ± 1.12 | 2.20 ± 0.89 | 2.52 ± 0.90 |
+C Group | 4 weeks | 1.05 ± 0.82 | 1.83 ± 0.77 | 2.50 ± 0.54 |
-C Group | 4 weeks | 0.54 ± 0.61 | 0.87 ± 0.64 | 1.04 ± 0.63 |
Test Group | 12 weeks | 0.56 ± 0.64 | 0.99 ± 1.02 | 1.20 ± 1.08 |
+C Group | 12 weeks | 0.76 ± 0.97 | 0.90 ± 1.02 | 1.49 ± 0.96 |
-C Group | 12 weeks | 0.17 ± 0.27 | 0.38 ± 0.42 | 0.63 ± 0.62 |
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Pla, R.; Sanz-Esporrin, J.; Noguerol, F.; Vignoletti, F.; Gamarra, P.; Sanz, M. A Synthetic Bio-Absorbable Membrane in Guided Bone Regeneration in Dehiscence-Type Defects: An Experimental In Vivo Investigation in Dogs. Bioengineering 2023, 10, 841. https://doi.org/10.3390/bioengineering10070841
Pla R, Sanz-Esporrin J, Noguerol F, Vignoletti F, Gamarra P, Sanz M. A Synthetic Bio-Absorbable Membrane in Guided Bone Regeneration in Dehiscence-Type Defects: An Experimental In Vivo Investigation in Dogs. Bioengineering. 2023; 10(7):841. https://doi.org/10.3390/bioengineering10070841
Chicago/Turabian StylePla, Rafael, Javier Sanz-Esporrin, Fernando Noguerol, Fabio Vignoletti, Pablo Gamarra, and Mariano Sanz. 2023. "A Synthetic Bio-Absorbable Membrane in Guided Bone Regeneration in Dehiscence-Type Defects: An Experimental In Vivo Investigation in Dogs" Bioengineering 10, no. 7: 841. https://doi.org/10.3390/bioengineering10070841