Bovine Placentome-Derived Extracellular Matrix: A Sustainable 3D Scaffold for Cultivated Meat
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Bovine Placentome Tissue for Extraction
2.2. Extraction of Collagen from Bovine Placental Tissues
2.3. Extraction of Extracellular Matrix (ECM) from Bovine Placental Tissues
2.4. Characterization of Collagen & ECM
2.5. Production of Collagen and ECM Scaffolds
2.6. Scanning Electron Microscopy
2.7. Isolation and Characterization of Bovine Myoblasts
2.8. Culturing Bovine Myoblasts on Scaffolds
2.9. Monitoring Growth of Bovine Myoblasts in Scaffolds
2.10. Staining
2.10.1. Immunofluorescent Staining
2.10.2. CalceinAM Staining
2.11. Design of Steak Construct
2.12. Preparing PDMS Mold from 3D Printed Steak-Shaped Mold
2.13. Preparing BP-ECM for Steak-Shaped Scaffold
2.14. Pan Frying ECM Scaffolds Containing Bovine Myoblasts
2.15. Statistical Analysis
3. Results
3.1. Isolation of Collagen and ECM from Bovine Placentome
3.2. Myoblast Culture on Collagen and ECM Scaffolds
3.3. Making a Muscle Tissue Like Meat Construct
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bektas, C.; Lee, K.; Jackson, A.; Bhatia, M.; Mao, Y. Bovine Placentome-Derived Extracellular Matrix: A Sustainable 3D Scaffold for Cultivated Meat. Bioengineering 2024, 11, 854. https://doi.org/10.3390/bioengineering11080854
Bektas C, Lee K, Jackson A, Bhatia M, Mao Y. Bovine Placentome-Derived Extracellular Matrix: A Sustainable 3D Scaffold for Cultivated Meat. Bioengineering. 2024; 11(8):854. https://doi.org/10.3390/bioengineering11080854
Chicago/Turabian StyleBektas, Cemile, Kathleen Lee, Anisha Jackson, Mohit Bhatia, and Yong Mao. 2024. "Bovine Placentome-Derived Extracellular Matrix: A Sustainable 3D Scaffold for Cultivated Meat" Bioengineering 11, no. 8: 854. https://doi.org/10.3390/bioengineering11080854