Effects of Exogenous Regulation of PPARγ on Ovine Oocyte Maturation and Embryonic Development In Vitro
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Oocyte Collection and In Vitro Culture
2.2. Parthenogenesis Activation and Embryo Culture
2.3. Immunofluorescence
2.4. Fat Drop Dyeing
2.5. ROS and GSH Detection
2.6. Real-Time Quantitative RT-PCR
2.7. Statistical Analysis
3. Results
3.1. Effects of Increasing PPARγ Activity on Ovine Oocyte Maturation
3.2. Effects of Increasing PPARγ Activity on Sheep Paracrine Factors
3.3. Effects of Increasing PPARγ Activity on Oxidative Stress Level of Sheep Oocytes
3.4. Effects of Increasing PPARγ Activity on Lipid Content of Sheep Oocytes
3.5. Effects of Increasing PPARγ Activity on Lipid Metabolism-Related Genes in Sheep Oocytes
3.6. Effects of Increasing PPARγ Activity on Parthenogenetic Activated Embryo Development
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Secquence | Amplicon Size | GenBank Accession Number |
---|---|---|---|
β-actin | F: TCTCTTCCAGCCTTCCTTCCTG R: AGCACCGTGTTGGCGTAGA | 184 bp | NM_001009784.3 |
GDF9 BMP15 | F: GACGCCACCTCTACAACACT R: TCCACAACAGTAACACGATCCA F: GCCCAACCAATCACTTTCCTTC R: GCCACCAGAACTCAAGAACCT | 133 bp 192 bp | NC_056058.1 NM_001114767.2 |
OCT4 | F: CGTGGTCAGAGTGTGGTTCT R: TGGCTTCAGAGGAAAGGATACG | 133 bp | NC_003074.8 |
SOX2 | F: ACATGAACGGCTGGAGCAA R: GCGAGCTGGTCATAGAGTTGTA | 156 bp | NC_056054.1 |
NANOG | F: ACTGTCTCTCCTCTTCCTTCCT R: CTCTTCCTTCTCTGTGCTCTCC | 120 bp | NC_056056.1 |
CPT2 | F: GCCACCTATGAGTCCTGTAGC R: CATCGCTGCTTCTCTGGTCA | 195 bp | NC_056054.1 |
ACSL1 | F: TCTGGATAAGGACGGCTGGTT R: AGGTTCACTTCGCTGGTAGATG | 160 bp | NC_056079.1 |
ACSL3 ACAA1 | F: ACCATCGCCATCTTCTGTGAA R: GGACCGCCTAGAGTAGCATAC F: AGGAGGTCCAAGGCAGAAGAG R: TCCACATCGTCCACCGTCAG | 107 bp 155 bp | NC_056055.1 NC_056072.1 |
ACADM ACADL | F: GTGGAGGTCTTGGACTTGGAA R: ACACAATGGCTCCTCAGTCAT F: TGTGACACTGTGATCGTCGTAG R: CTGCTGGCAACCGTACATCT | 182 bp 184 bp | NC_056054.1 NC_056055.1 |
CPT1A | F: TCACATCCAGGCGGCAAGA R: GAGCAGAGCGGAATCGTAGAC | 120 bp | NC_056074.1 |
CPT1B | F: TGCGTTCTTCGTGGCTCTG R: GCGTGCTCTGTGTTGAGTC | 177 bp | NC_056056.1 |
CPT1C | F: AATCCACCATGACTCGCTTGT R: CCACTCATCGCTGCCTTCA | 181 bp | NC_056067.1 |
Group | Number of Oocytes | PB Rate(%) |
---|---|---|
0 | 121 | 43.52 ± 5.03 b |
5 μM | 123 | 54.29 ± 5.13 a |
10 μM | 106 | 43.86 ± 4.51 b |
20 μM | 99 | 40.33 ± 5.57 b |
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Yu, H.; Zhang, Y.; Zhang, Y.; Chen, S.; Li, Z.; Pi, W.; Zeng, W.; Hu, G. Effects of Exogenous Regulation of PPARγ on Ovine Oocyte Maturation and Embryonic Development In Vitro. Vet. Sci. 2024, 11, 397. https://doi.org/10.3390/vetsci11090397
Yu H, Zhang Y, Zhang Y, Chen S, Li Z, Pi W, Zeng W, Hu G. Effects of Exogenous Regulation of PPARγ on Ovine Oocyte Maturation and Embryonic Development In Vitro. Veterinary Sciences. 2024; 11(9):397. https://doi.org/10.3390/vetsci11090397
Chicago/Turabian StyleYu, Hengbin, Yue Zhang, Yidan Zhang, Shuaitong Chen, Zhenghang Li, Wenhui Pi, Weibin Zeng, and Guangdong Hu. 2024. "Effects of Exogenous Regulation of PPARγ on Ovine Oocyte Maturation and Embryonic Development In Vitro" Veterinary Sciences 11, no. 9: 397. https://doi.org/10.3390/vetsci11090397