Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Previous Studies
2.2. Escherichia coli Collections
2.3. Genomic O Serotyping
2.4. Whole Genome Sequencing
2.5. Phylogenetic Tree
3. Results
3.1. Genomic O Serotyping
3.2. Identification of O:H Serotypes, MLSTypes, and Eae Gene Subtypes by WGS
3.3. Identification of Virulotypes by WGS
3.4. Comparison of Serotypes, ST, and Virulotypes of EPEC from Diarrheic Calves, Healthy Cattle, and Healthy Calves
3.5. Phylogenetic Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer | Sequence 5′-3′ | Amplicon Size (bp) | Reference |
---|---|---|---|---|
O123/186 | O123/186-F | TTTCAACAGGTTCGAATGCC | 362 | [8] |
O123/186-R | CCCACCAATACCACTGGAATA | |||
O156 | O156-F | GGAAAATGGAACATTTAGCGG | 236 | [8] |
O156-R | TCGGAGTGCCAACCAAAATA | |||
O177 | O177-F | CCGATACACCGGATGGATTAT | 427 | [8] |
O177-R | AAGCCAGTACCCAGAACAGGA | |||
O182 | O182-F | CGGTGATGGTTCTATTCTTGG | 502 | [8] |
O182-R | TGCTTGCACCAACTGTGTTA | |||
O183 | O183-F | CGTGGTAACCAATTTCGCAA | 666 | [8] |
O183-R | GGGAATAACGAACGGTTTACA |
O:H Serotype (Nr. Isolates) | MLST (Nr. Isolates) | Host (Nr. Isolates) | Virulotype (Nr. Isolates) | LEE-Located T3SS- and T3SE-Encoding Genes: Nr. +Ve Isolates | Non-LEE-Encoded T3SE-Encoding Genes: Nr. +Ve Isolates | Other Virulence Genes: Nr. +Ve Isolates | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
espA/espF/tir/espP | espB | nleA/B/C/espJ | cif | espI | gad/lpfA/ehxA | etpD | iss/tccP | efa1/astA | katP/iha/celB | ||||
O156:H8 (3) | ST327 (3) | HC (3) | eaeθ (3) b | 0 to 3 | 3° | 0 to 3 | 3 | 0 | 3 | 0 | 0 to 3 | 0–1 | 0 |
O156:H25 (12) | ST300 (11) | HC (11) | eaeζ (10) c,d | 10 | 10° | 10 | 0 | 0 | 10 | 10 | 6 | 0 | 0 |
eaeζ, stx2g (1) c | 1 | 1° | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | |||
ST4942 (1) | HU (1) | eaeζ, stx1a (1) | 1 | 1° | 1 | 0 | 0 | 1 | 1 | 1 | 0–1 | 0 | |
O177:H11 (17) | ST765 (15) | DC (8) | eaeβ (8) | 8 | 8 | 2 to 8 | 8 | 8 | 7 | 0 | 4 to 7 | 7 | 0–1 |
HC (1) | eaeβ (1) | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | ||
HCS (6) | eaeβ, stx1a (1) | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | ||
eaeβ, stx2c (1), | 1 | 0 | 1 | 1 | 0 | ||||||||
stx2c (1) | 1 | 1 | 1 | 1 | 1 | 4 | 0 | 4 | 4 | 0 | |||
ST29 (2) | DC (1) | eaeβ (4) e | 4 | 4 | 2 to 4 | 4 | 4 | 1 | 0 | 0–1 | 1 | 0–1 | |
HU (1) | eaeβ (1) | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0–1 | ||
eaeβ, stx1a (1) | 1 | 1 | 1 | 0 | 0 | ||||||||
O177:H25 (2) | ST342 (2) | DC (2) | eaeβ (1) | 0–1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 |
eaeβ, stx2c (1) | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | |||
0O182:H25 (10) | ST300 (10) | DC (4) | eaeζ (2) | 2 | 2° | 2 | 0 | 0 | 2 | 2 | 2 | 1 | 0 |
eaeζ, stx1a (2) | 2 | 2° | 2 | 0 | 0 | 2 | 2 | 2 | 0–2 | 0–1 | |||
HC (2) | eaeζ (2) | 2 | 2° | 2 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | ||
HCS (2) | eaeζ (2) f | 2 | 2° | 0–2 | 0 | 0 | 2 | 1–2 | 2 | 1–2 | 0 | ||
HU (2) | eaeζ, stx1a (2) | 2 | 2° | 1–2 | 0 | 0 | 2 | 2 | 0–2 | 0 | 0 |
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Habets, A.; Touzain, F.; Lucas, P.; Huong, N.T.T.; Iguchi, A.; Crombé, F.; Korsak, N.; Piérard, D.; Saulmont, M.; Cox, E.; et al. Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli. Vet. Sci. 2022, 9, 492. https://doi.org/10.3390/vetsci9090492
Habets A, Touzain F, Lucas P, Huong NTT, Iguchi A, Crombé F, Korsak N, Piérard D, Saulmont M, Cox E, et al. Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli. Veterinary Sciences. 2022; 9(9):492. https://doi.org/10.3390/vetsci9090492
Chicago/Turabian StyleHabets, Audrey, Fabrice Touzain, Pierrick Lucas, Nguyen Thi Thu Huong, Atsushi Iguchi, Florence Crombé, Nicolas Korsak, Denis Piérard, Marc Saulmont, Eric Cox, and et al. 2022. "Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli" Veterinary Sciences 9, no. 9: 492. https://doi.org/10.3390/vetsci9090492