MaAzaR Influences Virulence of Metarhizium acridum against Locusta migratoria manilensis by Affecting Cuticle Penetration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Bioassays
2.3. RNA Isolation, RT-qPCR, and RNA Sequencing
2.4. Cuticle Penetration Simulation
2.5. Appressorium Formation and Turgor Pressure Measurement
2.6. Phenoloxidase Assay
2.7. In Vitro Culture of Hyphal Bodies and its DNA Content Quantification
2.8. Data Analysis
3. Results
3.1. Disruption of MaAzaR Reduces the Virulence of M. acridum by Impeding Cuticle Penetration
3.2. MaAzaR Influences Cuticle Penetration Process by Affecting Appressoria
3.3. Disruption of MaAzaR Induces Higher Host Immune Responses During Cuticle Penetration
3.4. The Absence of MaAzaR Does Not Impair In Vivo Growth Stage of M. acridum
3.5. Transcriptomic Insights into the Roles of MaAzaR in Appressorium Formation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hong, G.; Wang, S.; Xia, Y.; Peng, G. MaAzaR Influences Virulence of Metarhizium acridum against Locusta migratoria manilensis by Affecting Cuticle Penetration. J. Fungi 2024, 10, 564. https://doi.org/10.3390/jof10080564
Hong G, Wang S, Xia Y, Peng G. MaAzaR Influences Virulence of Metarhizium acridum against Locusta migratoria manilensis by Affecting Cuticle Penetration. Journal of Fungi. 2024; 10(8):564. https://doi.org/10.3390/jof10080564
Chicago/Turabian StyleHong, Geng, Siqing Wang, Yuxian Xia, and Guoxiong Peng. 2024. "MaAzaR Influences Virulence of Metarhizium acridum against Locusta migratoria manilensis by Affecting Cuticle Penetration" Journal of Fungi 10, no. 8: 564. https://doi.org/10.3390/jof10080564