Fungal Identifier (FId): An Updated Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Approach to Ease Ascomycetous Yeast Isolates’ Identification in Ecological Studies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ascomycete Genomes and Sequence Retrieval
2.2. In Silico Endonuclease Restriction
2.3. Valuation of the Experimental Error and Calibration of the Model
2.4. Testing the Model with Experimental Data
2.5. Identification of Yeast Species with Experimental Error
2.6. Testing the Combination of Multiple Profiles
3. Results
3.1. Sequence Database
3.2. Restriction Fragments Profiles Obtained with Endonucleases In Silico
3.3. Analysis of Experimental Data
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abbà, S.; Valentini, B.; Stefanini, I. Fungal Identifier (FId): An Updated Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Approach to Ease Ascomycetous Yeast Isolates’ Identification in Ecological Studies. J. Fungi 2024, 10, 595. https://doi.org/10.3390/jof10090595
Abbà S, Valentini B, Stefanini I. Fungal Identifier (FId): An Updated Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Approach to Ease Ascomycetous Yeast Isolates’ Identification in Ecological Studies. Journal of Fungi. 2024; 10(9):595. https://doi.org/10.3390/jof10090595
Chicago/Turabian StyleAbbà, Silvia, Beatrice Valentini, and Irene Stefanini. 2024. "Fungal Identifier (FId): An Updated Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Approach to Ease Ascomycetous Yeast Isolates’ Identification in Ecological Studies" Journal of Fungi 10, no. 9: 595. https://doi.org/10.3390/jof10090595