Identification of Clinical Isolates of Candida albicans with Increased Fitness in Colonization of the Murine Gut
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Genetic Procedures
2.3. In Vivo Procedures
2.4. NGS Assay
2.5. C. albicans Filamentation Assay
2.6. Supplementary Material and Methods
2.6.1. Susceptibility Assays
2.6.2. Adhesion Assay
2.6.3. Phospholipase Activity
2.7. C. albicans Susceptibility Testing
3. Results
3.1. Identification of Clinical Isolates of Candida albicans with Increased Gut Colonization Load
3.2. Selected C. albicans Strains Displayed Enhanced Fitness Compared to SC5314
3.3. Strains with Increased Fitness Are Able to Form True Hyphae
3.4. CaORAL3 Strain Still Requires Antibiotic Treatment to Colonize Murine Gut
3.5. Changes in the Intestinal Microbiota Allow CaORAL3 Colonization
3.6. CaORAL3 Is Able to Colonize Murine Gut Previously Colonized with C. albicans
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain Name | Strain Background and Genotype | Reference |
---|---|---|
SC5314-RFP | SC5314 ADH1/adh1::SAT1-dTOM2 | This work |
CaORAL3-RFP | CaORAL3 ADH1/adh1::SAT1-dTOM2 | This work |
Strain | Origin | Sample |
---|---|---|
Practs 2018-1 | Commensal | Stool culture |
CaORAL2 | Hospital | Oral cavity |
CaORAL3 | Hospital | Oral cavity |
CaHG14 | Hospital | Bronchoalveolar lavage |
CaHG22 | Hospital | Pharyngeal exudate |
CaHG43 | Hospital | Pharyngeal exudate |
CaHG65 | Hospital | Urine culture |
CaURI1 | Hospital | Urine culture |
Strain | Invasiveness in Spider Medium | Adhesion to Polyestyrene | Bile Salts Sensitivity | Menadione | Phospholipase Activity (SEA) |
---|---|---|---|---|---|
Pract. 2018-1 | n.s. | n.s. | R | R | n.s. |
CaORAL2 | n.s. | n.s. | R | n.s. | n.s. |
CaORAL3 | n.s. | < | R | n.s. | n.s. |
CaHG14 | Non invasive | n.s. | n.s. | R | n.s. |
CaHG22 | n.s. | n.s. | S | n.s. | ↓ |
CaHG43 | n.s. | n.s. | n.s. | n.s. | +++ |
CaHG65 | n.s. | n.s. | n.s. | n.s. | ↓ |
CaURI1 | Non invasive | n.s. | n.s. | R | ++ |
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Alonso-Monge, R.; Prieto, D.; Coman, I.; Rochas, S.; Arana, D.M.; Hidalgo-Vico, S.; Román, E.; Pla, J. Identification of Clinical Isolates of Candida albicans with Increased Fitness in Colonization of the Murine Gut. J. Fungi 2021, 7, 695. https://doi.org/10.3390/jof7090695
Alonso-Monge R, Prieto D, Coman I, Rochas S, Arana DM, Hidalgo-Vico S, Román E, Pla J. Identification of Clinical Isolates of Candida albicans with Increased Fitness in Colonization of the Murine Gut. Journal of Fungi. 2021; 7(9):695. https://doi.org/10.3390/jof7090695
Chicago/Turabian StyleAlonso-Monge, Rebeca, Daniel Prieto, Ioana Coman, Sara Rochas, David M. Arana, Susana Hidalgo-Vico, Elvira Román, and Jesús Pla. 2021. "Identification of Clinical Isolates of Candida albicans with Increased Fitness in Colonization of the Murine Gut" Journal of Fungi 7, no. 9: 695. https://doi.org/10.3390/jof7090695