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Communication

Three New Species of Fusicolla (Hypocreales) from China

by
Zhao-Qing Zeng
* and
Wen-Ying Zhuang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
J. Fungi 2023, 9(5), 572; https://doi.org/10.3390/jof9050572
Submission received: 8 April 2023 / Revised: 11 May 2023 / Accepted: 13 May 2023 / Published: 15 May 2023
(This article belongs to the Special Issue Ascomycota: Diversity, Taxonomy and Phylogeny, 2nd Edition)
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Abstract

:
To explore the species diversity of the genus Fusicolla, specimens from Henan, Hubei and Jiangsu Provinces in China are examined, and three undescribed taxa are encountered. The morphological characteristics and DNA sequence analyses of the combined acl1, ITS, LSU, rpb2 and tub2 regions support their placement in Fusicolla and their recognition as new species. Fusicolla aeria sp. nov. is distinguished by the formation of abundant aerial mycelia on PDA, falcate, (1–)3-septate macroconidia 16–35 × 1.5–2.8 μm and subcylindrical, aseptate microconidia 7.5–13 × 0.8–1.1 μm. Fusicolla coralloidea sp. nov. has a coralloid colony on PDA, falcate, 2–5-septate macroconidia 38–70 × 2–4.5 μm and rod-shaped to ellipisoidal, aseptate microconidia 2–7 × 1–1.9 μm. Fusicolla filiformis sp. nov. is characterized by filiform, 2–6-septate macroconidia 28–58 × 1.5–2.3 μm and lacking microconidia. Morphological differences between these novel species and their close relatives are compared in detail. The previously recorded species of the genus in China are listed and a key to these taxa is provided.

1. Introduction

The genus Fusicolla Bonord., typified by F. betae (Desm.) Bonord., was established by Bonorden [1] and redefined by Gräfenhan et al. [2], who raised the varieties of Fusarium aquaeductuum (Radlk. & Rabenh.) Lagerh. & Rabenh. to species rank and transferred Fusarium merismoides Corda to Fusicolla. The genus is characterized by scattered to gregarious, yellow, pale buff to orange, globose to pyriform perithecia that are fully or partially immersed in stromata; cylindrical to narrowly clavate asci containing eight ascospores; and the production of falcate, straight to curved, 1–5-septate macroconidia [2,3]. They are mostly saprobes and occur on various substrata, such as rotten twigs, decayed wood, the stromata of other fungi, soil, water, the slime flux of trees, sewage, the bones of wild boar and even air [2,4,5,6,7,8,9]. Currently, there are 22 species accepted in this genus [9,10], of which five are reported from China [9,11,12,13].
Within the scope of our current study on the Chinese Fungus Flora, fresh hypocrealean specimens are examined. Based on the morphology and phylogenetic analyses of the combined sequences of the larger subunit of the ATP citrate lyase (acl1), nuclear ribosomal DNA ITS1-5.8S-ITS2 (ITS), the large subunit of nuclear ribosomal DNA (LSU), the second largest subunit of RNA polymerase II (rpb2) and β-tubulin (tub2), three novel species of Fusicolla are introduced. Comparisons between these taxa and their close relatives are performed. The previously recorded Fusicolla species in China are also listed.

2. Materials and Methods

2.1. Sampling and Morphological Studies

Specimens on wood substrates were collected from Henan, Hubei and Jiangsu Provinces in China and deposited in the Herbarium Mycologicum Academiae Sinicae (HMAS). Lactophenol cotton blue solution was used as a mounting medium for the examination of features and measurements of conidiophores, macroconidia and microconidia. Photographs were taken with a Zeiss AxioCam MRc 5 digital camera (Jena, Germany) attached to a Zeiss Axio Imager A2 microscope (Göttingen, Germany). Cultures were deposited in the China General Microbiological Culture Collection Center (CGMCC). For colony features and growth rates, strains were grown on potato dextrose agar (PDA, 20% (w/v) potato + 2% (w/v) dextrose + 2% (w/v) agar) and synthetic nutrient-poor agar (SNA) [14] in 90 mm plastic Petri dishes at 25 °C for 14 d with alternating periods of light and darkness (12 h/12 h).

2.2. DNA Extraction, PCR Amplification, Sequencing and Phylogenetic Analyses

Genomic DNA was extracted from fresh mycelium following the method of Wang and Zhuang [15]. Five primer pairs, acl1-230up/acl1-1220low [16], ITS5/ITS4 [17], LR0R/LR5 [18,19], RPB2-5f/RPB2-7cR [20] and T1/T22 [21], were used to amplify the sequences of the acl1, ITS, LSU, rpb2 and tub2 regions, respectively. PCR reactions were performed using an ABI 2720 Thermal Cycler (Applied Biosciences, Foster City, CA, USA) with a 25 μL reaction mixture consisting of 12.5 μL Taq MasterMix, 1 μL of each primer (10 μM), 1 μL template DNA and 9.5 μL ddH2O. DNA sequencing was carried out in both directions on an ABI 3730XL DNA Sequencer (Applied Biosciences, Foster City, CA, USA).
Newly acquired sequences and those retrieved from GenBank are listed in Table 1. The sequences were assembled and aligned, and the primer sequences were trimmed by BioEdit 7.0.5 [22] and converted to NEXUS files by ClustalX 1.83 [23]. The sequences of acl1, ITS, LSU, rpb2 and tub2 were combined and analyzed by Bayesian inference (BI) and maximum likelihood (ML) methods to determine the phylogenetic positions of these strains. The BI analysis was conducted by MrBayes 3.1.2 [24] using a Markov chain Monte Carlo (MCMC) algorithm. Nucleotide substitution models were determined by MrModeltest 2.3 [25]. The ML analysis was performed via IQ-Tree 1.6.12 [26] using the best model for each locus chosen by ModelFinder [27]. Trees were examined by TreeView 1.6.6 [28]. The Bayesian inference posterior probability (BIPP) values greater than 0.9 and maximum likelihood bootstrap (MLBP) values greater than 70% were shown at the nodes.

3. Results

3.1. Phylogeny

The acl1, ITS, LSU, rpb2 and tub2 sequences of 24 Fusicolla species were analyzed. The resulting BI tree is shown in Figure 1. The topology of the ML tree was similar to that of the BI tree. The final matrix was deposited in TreeBASE with accession no. S30023. The isolates CGMCC 3.24907, 3.24908, 3.24909 and 3.24910 grouped with other members of Fusicolla, and the genus received high statistical support values (BIBP/MLBP = 1.0/96%). The isolate CGMCC 3.24910 clustered together with F. gigas Chang Liu, Z.Q. Zeng & W.Y. Zhuang (BIBP/MLBP = 1.0/100%). The isolates CGMCC 3.24908 and 3.24909 were related to F. acetilerea (Tubaki, C. Booth & T. Harada) Gräfenhan & Seifert and F. elongata Decock, Crous & Sand.-Den. but with low support values, and the isolate CGMCC 3.24907 formed a separate lineage.

3.2. Taxonomy

  • Fusicolla aeria Z.Q. Zeng & W.Y. Zhuang, sp. nov., Figure 2.
Jof 09 00572 g002 550
Figure 2. Fusicolla aeria (CGMCC 3.24908). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c–e) conidiophores and macroconidia; (f–i) macroconidia; (j) conidiophores and microconidia; (k–n) microconidia. Bars: 10 μm.
Figure 2. Fusicolla aeria (CGMCC 3.24908). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c–e) conidiophores and macroconidia; (f–i) macroconidia; (j) conidiophores and microconidia; (k–n) microconidia. Bars: 10 μm.
Jof 09 00572 g002
Fungal Names: FN 571312.
Etymology: The specific epithet refers to the abundant aerial mycelium on PDA.
Typification: CHINA, Henan Province, Luoyang, Yushan Forest Park, 34°41′23″ N 112°6′13″ E, on rotten twig, 23 September 2013, H.D. Zheng, Z.Q Zeng & Z.X. Zhu 8875 (holotype HMAS 247866, ex-type strain CGMCC 3.24908). Sequences: acl1 OQ134105, ITS OQ128334, LSU OQ128338, rpb2 OQ134111, tub2 OQ134100.
Other specimen examined: CHINA, Henan Province, Jiaozuo, Yuntaishan, 35°25′53″ N 113°23′30″ E, on twig associated with other fungi, 25 September 2013, H.D. Zheng, Z.Q. Zeng & Z.X. Zhu 8916a (HMAS 247867, strain CGMCC 3.24909). Sequences: acl1 OQ134106, ITS OQ128335, LSU OQ128339, rpb2 OQ134112, tub2 OQ134101.
Colony characteristics: On PDA 35 mm diam. after 2 wk at 25 °C, with abundant, orange aerial mycelium, producing pinkish orange pigment. On SNA 40 mm diam. after 2 wk at 25 °C, with sparse, pale greyish-white aerial mycelium. Conidiophores unbranched or simple branched, hyaline, smooth-walled, septate, bearing terminal and lateral conidiogenous cells. Conidiogenous cells monophialidic, cylindrical to conical, 18−40 × 1.5−3 µm, smooth, thin-walled. Macroconidia falcate, straight to slightly curved, slightly hooked at one end, hyaline, smooth, (1–)3-septate, 16–35 × 1.5–2.8 μm. Microconidia aseptate, subcylindrical, curved to C-shaped, smooth, hyaline, 7.5–13 × 0.8–1.1 μm. Chlamydospores absent. Sexual stage not observed.
Notes: Among the known species of the genus, F. aeria is distinct because of its abundant aerial mycelium on PDA. Morphologically, it resembles F. gigas and F. matuoi (Hosoya & Tubaki) Gräfenhan & Seifert in having C-shaped microconidia in culture. However, F. gigas possesses larger macroconidia (32−80 × 2.3–3.8 µm) with more septa (3–9 septa) [9], while F. matuoi forms longer macroconidia (17–56 μm long) [29]. Phylogenetically, they are remotely related (Figure 1).
  • Fusicolla coralloidea Z.Q. Zeng & W.Y. Zhuang, sp. nov., Figure 3.
Jof 09 00572 g003 550
Figure 3. Fusicolla coralloidea (CGMCC 3.24907). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c–e) conidiophores, macroconidia and microconidia; (f,g) conidiophores and macroconidia; (h–l) macroconidia and microconidia; (m,n) microconidia. Bars: 10 μm.
Figure 3. Fusicolla coralloidea (CGMCC 3.24907). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c–e) conidiophores, macroconidia and microconidia; (f,g) conidiophores and macroconidia; (h–l) macroconidia and microconidia; (m,n) microconidia. Bars: 10 μm.
Jof 09 00572 g003
Fungal Names: FN 571313.
Etymology: The specific epithet refers to the coralloid colony on PDA.
Typification: CHINA, Jiangsu Province, Nanjing City, campus of Nanjing Normal University, 32°6′44″ N 118°55′ E, on twig associated with other fungi, 25 July 2011, Z.Q. Zeng & H.D. Zheng 7895 (holotype HMAS 247870, ex-type strain CGMCC 3.24907). Sequences: acl1 OQ134104, ITS OQ128333, LSU OQ128337, rpb2 OQ134110, tub2 OQ134099.
Colony characteristics: On PDA 34 mm diam. after 2 wk at 25 °C, forming coralloid synnema on surface, producing pale orange-yellow pigment. On SNA 32 mm diam. after 2 wk at 25 °C, with very sparse, pale greyish-white aerial mycelium. Conidiophores arising from somatic hyphae, hyaline, smooth-walled, septate, bearing terminal and lateral conidiogenous cells. Conidiogenous cells monophialidic, cylindrical to conical, 18−60 × 2−3 µm, smooth, thin-walled. Macroconidia falcate, straight to slightly curved, acute at both ends, slightly hooked at one end, hyaline, smooth, 2–5-septate, 38–70 × 2–4.5 μm. Microconidia aseptate, rod-shaped to ellipisoidal, straight to slightly curved, hyaline, smooth, 2–7 × 1–1.9 μm. Chlamydospores absent. Sexual stage not observed.
Note: Among the known species of Fusicolla, F. coralloidea is distinguished by the production of coralloid synnemata on the PDA surface. The fungus resembles F. epistroma (Höhn.) Gräfenhan & Seifert in having rod-shaped to ellipisoidal microconidia [2]. However, the microconidia of the latter are much longer (3.5–8 μm long). Phylogenetically, they were recognized as distinct lineages (Figure 1). Both morphology and DNA sequence analyses support the independent status of these species.
  • Fusicolla filiformis Z.Q. Zeng & W.Y. Zhuang, sp. nov., Figure 4.
Jof 09 00572 g004 550
Figure 4. Fusicolla filiformis (CGMCC 3.24910). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c,d) conidiophores and macroconidia; (e–p) macroconidia. Bars: 10 μm.
Figure 4. Fusicolla filiformis (CGMCC 3.24910). (a) Colony after 2 wk at 25 °C on PDA; (b) colony after 2 wk at 25 °C on SNA; (c,d) conidiophores and macroconidia; (e–p) macroconidia. Bars: 10 μm.
Jof 09 00572 g004
Fungal Names: FN 571314.
Etymology: The specific epithet refers to its filiform macroconidia.
Typification: CHINA, Hubei Province, Shennongjia Forestry District, Muyu Town, 31°24′55″ N 110°28′55″ E, on rotten twig, 25 October 2021, Z.Q. Zeng, Z.H. Yu & J.X. Deng 12994b (holotype HMAS 247871, ex-type strain CGMCC 3.24910). Sequences: acl1 OQ134103, ITS OQ128332, LSU OQ128336, rpb2 OQ134109, tub2 OQ134098.
Colony characteristics: On PDA 20 mm diam. after 2 wk at 25 °C, with very sparse, orange aerial mycelium, producing orange pigment. On SNA 17 mm diam. after 2 wk at 25 °C, with very sparse, pale yellowish-white aerial mycelium. Conidiophores arising from somatic hyphae, unbranched, hyaline, smooth-walled, septate, bearing terminal and lateral conidiogenous cells. Conidiogenous cells monophialidic, cylindrical to conical, 25–62 × 1.8–2.5 μm, smooth, thin-walled. Macroconidia filiform to falcate, straight to slightly curved, acute at both ends, with hooked cell at one end, hyaline, smooth, 2–6-septate, 28–58 × 1.5–2.3 μm. Microconidia and chlamydospores absent. Sexual stage not observed.
Note: Phylogenetically, F. filiformis clustered with F. gigas, receiving full support (Figure 1). However, between their type cultures, there are 25 bp, 7 bp, 9 bp, 39 bp and 21 bp divergences detected for acl1, ITS, LSU, rpb2 and tub2 regions, respectively. Morphologically, F. gigas differs in having C-shaped microconidia and wider macroconidia (2.5–3.5 μm wide) with more septa (up to nine septa) [9].
  • Other Fusicolla Species Recorded in China
  • Fusicolla aquaeductuum (Radlk. & Rabenh.) Gräfenhan, Seifert & Schroers, in Gräfenhan, Schroers, Nirenberg & Seifert, Stud. Mycol. 68: 100, 2011.
≡ Selenosporium aquaeductuum Radlk. & Rabenh., in Rabenhorst, Hedwigia 2: 73, 1862.
≡ Fusarium aquaeductuum (Radlk. & Rabenh.) Lagerh. & Rabenh., Centbl. Bakt. ParasitKde, Abt. I 9: 655. 1891.
Distribution: China, Germany and Netherlands [2,12].
  • Fusicolla gigas Chang Liu, Z.Q. Zeng & W.Y. Zhuang, in Crous et al., Fungal Systematics and Evolution 9: 192, 2022.
Specimen examined: CHINA, Chongqing City, Wushan County, Hongchiba National Forest Park, in soil, 30 October 2020, Z.Q. Zeng, X.C. Wang, H.D. Zheng & C. Liu CGMCC 3.20680 (HMAS 247872).
Distribution: China [9,10].
  • Fusicolla guangxiensis Z.Q. Zeng, Chang Liu & W.Y. Zhuang, in Crous et al., Fungal Systematics and Evolution 9: 192, 2022.
Specimen examined: CHINA, Guangxi Zhuang Autonomous Region, Fangchenggang City, Shiwandashan National Forest Park, on rotten twig, 10 December 2019, Z.Q. Zeng & H.D. Zheng CGMCC 3.20679 (HMAS 247873).
Distribution: China [9,10].
  • Fusicolla matuoi (Hosoya & Tubaki) Gräfenhan & Seifert, in Gräfenhan, Schroers, Nirenberg & Seifert, Stud. Mycol. 68: 101, 2011.
≡ Fusarium matuoi Hosoya & Tubaki, Mycoscience 45: 264, 2004.
Distribution: China, Iran and Japan [2,11].
  • Fusicolla violacea Gräfenhan & Seifert, in Gräfenhan, Schroers, Nirenberg & Seifert, Stud. Mycol. 68: 101, 2011.
= Fusarium merismoides var. violaceum Gerlach, Phytopath. Z. 90(1): 34, 1977. Nom. inval., Art. 37.
Distribution: China and Iran [2,13].
  • Key to the Known Species of Fusicolla in China
1. Forming macroconidia and microconidia on PDA2
1. Only forming macroconidia on PDA6
  2. Microconidia ellipiosoid, rod-shaped to falcate3
  2. Microconidia subcylindrical, curved to C-shaped4
3. Producing pale orange-yellow pigment on PDAF. coralloidea
3. Producing purple pigment on PDAF. violacea
  4. Aerial mycelium abundant on PDAF. aeria
  4. Aerial mycelium absent to spare on PDA5
5. Colony on PDA light yellow to deep orangeF. matuoi
5. Colony on PDA pinkish orangeF. gigas
  6. Macroconidia filiformF. filiformis
  6. Macroconidia falcate7
7. Producing orange-yellow pigment on PDAF. guangxiensis
7. Producing pink pigment on PDAF. aquaeductuum

4. Discussion

Since the establishment of Fusarium Link in 1809, many fusarioid species have been assigned to the genus and the generic boundary has become obscure. The accumulated morphological and phylogenetic data suggested that the genus was heterogeneous [30]. Efforts were made toward the construction of a monophyletic Fusarium as well as its allies [31,32]. The previously recognized members classified in Fusarium sensu lato are now treated as separate genera, i.e., Albonectria Rossman & Samuels, Atractium Link, Bisifusarium L. Lombard, Crous & W. Gams, Cosmosporella S.K. Huang, R. Jeewon & K.D. Hyde, Cyanonectria Samuels & P. Chaverri, Dialonectria (Sacc.) Cooke, Fusicolla, Geejayessia Schroers, Gräfenhan & Seifert, Macroconia (Wollenw.) Gräfenhan, Seifert & Schroers, Microcera Desm., Neocosmospora E.F. Sm., Pseudofusicolla D. Triest, Rectifusarium (L. Lombard, Crous & W. Gams) and Stylonectria Höhn. [2,33,34].
Several studies have shown that members of Fusicolla are economically important in the fields of human health [11,35,36,37,38], fermentation [39,40], ecology [41,42] and agriculture [13,43,44,45]. For example, Fusicolla species were related to gastric cancer and disorganized lipid metabolism in patients with nonalcoholic fatty liver disease [37,38]. Fusicolla merismoides (Corda) Gräfenhan, Seifert & Schroers (as Fusarium merismoides Corda) was reported as an important source of anticancer agents [35], and F. violacea can produce secondary bioactive metabolites that may be potential biological agents [13,43]. Thus, studies on the biodiversity of Fusicolla are of theoretical and practical importance and should be continuously and extensively carried out.
The phylogenetic overview of Fusicolla based on multilocus sequence analyses showed that the genus is monophyletic [2]. The present phylogeny, including the newly added taxa, inferred from sequences of the acl1, ITS, LSU, rpb2 and tub2 regions, resulted in a similar tree topology to that demonstrated in the previous studies [8,9,46,47]. The result indicated that the four Chinese strains (CGMCC 3.24907, 3.24908, 3.24909 and 3.24910) grouped with the known species of Fusicolla (BIBP/MLBP = 1.0/96%), which confirmed their taxonomic placements. Fusicolla filiformis is associated with, but clearly separated from, F. gigas (BIBP/MLBP = 1.0/100%) and is characterized by filiform macroconidia. Fusicolla aeria is grouped with F. acetilerea and F. elongata, all three species forming abundant aerial mycelia on PDA. Fusicolla coralloidea, representing an independent linage, can be easily distinguished by its coralloid synnemata in culture and rod-shaped to ellipsoidal microconidia.
Among the known species of Fusicolla, F. aquaeductuum, F. betae, F. bharatavarshae Devadatha, V.V. Sarma & E.B.G. Jones, F. epistroma, F. melogrammae Lechat & Aplin, F. ossicola Lechat & Rossman and F. siamensis R.H. Perera, E.B.G. Jones & K.D. Hyde were described with both sexual and asexual stages [2,4,5,12,47,48]. However, F. cassiae-fistulae R.H. Perera, E.B.G. Jones & K.D. Hyde, F. gigantispora Dayar. & K.D. Hyde and F. reyesiana (Sacc.) Forin & Vizzini are only known from their sexual stages, and the remaining taxa are reported solely with their asexual stages [2,5,8,10,34,46,47,49], as well as the newly described species. Large-scale surveys covering different ecosystems and substrates in unexplored regions will further improve our knowledge of the species diversity of the genus and establish connections between the sexual and asexual stages of Fusicolla species, which will permit a better understanding of the whole fungus.

Author Contributions

Conceptualization, Z.-Q.Z. and W.-Y.Z.; methodology, software, validation, formal analysis, investigation, Z.-Q.Z.; resources, W.-Y.Z. and Z.-Q.Z.; data curation, Z.-Q.Z.; writing—original draft preparation, Z.-Q.Z.; writing—review and editing, W.-Y.Z. and Z.-Q.Z.; visualization, Z.-Q.Z.; supervision, Z.-Q.Z. and W.-Y.Z.; project administration, W.-Y.Z.; funding acquisition, W.-Y.Z. and Z.-Q.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by the National Natural Science Foundation of China (32270009, 31750001, 31870012); the Biological Resources Programme, Chinese Academy of Sciences (KFJ-BRP-017-082); and the Frontier Key Program of the Chinese Academy of Sciences (QYZDY-SSW-SMC029).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The names of the new species were formally registered in the database Fungal Names (https://nmdc.cn/fungalnames (accessed on 20 February 2023)). Specimens were deposited in the Herbarium Mycologicum Academiae Sinicae (https://nmdc.cn/fungarium/ (accessed on 18 February 2023)). Cultures were deposited in the China General Microbiological Culture Collection Center (https://cgmcc.net/ (accessed on 4 April 2023)). The newly generated sequences were deposited in GenBank (https://www.ncbi.nlm.nih.gov/genbank (accessed on 29 December 2022)).

Acknowledgments

The authors would like to thank Z.H. Yu, H.D. Zheng, J.X. Deng and Z.X. Zhu for collecting samples jointly for this study and to T. Huang for the technical help.

Conflicts of Interest

The authors declare no conflict of interest.

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Jof 09 00572 g001 550
Figure 1. The BI tree generated based on the combined datasets of acl1, ITS, LSU, rpb2 and tub2 sequences of Fusicolla species. BIPP (left) values greater than 0.9 and MLBP (right) values greater than 70% are shown at the nodes. Macroconia leptosphaeriae and Microcera larvarum were chosen as outgroup taxa.
Figure 1. The BI tree generated based on the combined datasets of acl1, ITS, LSU, rpb2 and tub2 sequences of Fusicolla species. BIPP (left) values greater than 0.9 and MLBP (right) values greater than 70% are shown at the nodes. Macroconia leptosphaeriae and Microcera larvarum were chosen as outgroup taxa.
Jof 09 00572 g001
Table
Table 1. List of Fusicolla species, herbarium/strain numbers and GenBank accession numbers of materials used in this study.
Table 1. List of Fusicolla species, herbarium/strain numbers and GenBank accession numbers of materials used in this study.
SpeciesHerbarium/Strain NumbersGenBank Accession Numbers
acl1ITSLSUrpb2tub2
F. acetilereaBBA 63789 THQ897839HQ897790U88108HQ897701−
F. aeriaCGMCC 3.24908 TOQ134105 aOQ128334 aOQ128338 aOQ134111 aOQ134100 a
CGMCC 3.24909OQ134106 aOQ128335 aOQ128339 aOQ134112 aOQ134101 a
F. aquaeductuumCBS 837.85 THQ897880KM231823KM231699HQ897744−
F. betaeBBA 64317 THQ897917−−HQ897781−
F. bharatavarshaeNFCCI 4423 T−MK152510MK152511MK157022MK376462
F. cassiae-fistulaeMFLUCC 19-0318 T−NR171299NG073862−−
F. coralloideaCGMCC 3.24907 TOQ134104 aOQ128333 aOQ128337 aOQ134110 aOQ134099 a
F. elongataCBS 148934 TON759286ON763203ON763200ON759297ON745628
F. epistromaBBA 62201 THQ897901−AF228352HQ897765−
F. filiformisCGMCC 3.24910 TOQ134103 aOQ128332 aOQ128336 aOQ134109 aOQ134098 a
F. gigantisporaMFLU 16-1206 T−MN047104MN017869−−
F. gigasCGMCC 3.20680 TOQ134107 aOK465362OK465449OQ134113 aOQ134102 a
F. guangxiensisCGMCC 3.20679 TOQ134108 aOK465363OK465450OQ134114 a−
F. hughesiiNFCCI 4234 T−MG779450MG779452−−
F. matuoiCBS 581.78 THQ897858KM231822KM231698HQ897720KM232093
F. melogrammaeCBS 141092 T−KX897140NG058275−MW834305
F. meniscoideaCBS 110189 TMW834043MW827613MW827654MW834010MW834306
F. merismoidesCBS 186.34 T−MH855482MH866963−−
F. ossicolaCBS 140161 T−NR161034MF628021MW834011MW834307
F. quarantenaeURM 8367 T−MW553789MW553788MW556626MW556624
F. septimanifiniscientiaeCBS 144935 T−MK069422MK069418−MK069408
F. siamensisMFLUCC 172577 T−NR171300NG073863−−
F. sporellulaCBS 110191 TMW834044MW827614MW827655MW834012MW834308
F. violaceaCBS 634.76 TKM231059KM231824KM231700HQ897696KM232095
Macroconia leptosphaeriaeCBS 100001HQ897891HQ897810KC291787HQ728164KM232097
Microcera larvarumCBS 738.79/AR 4580KM231060KM231825KM231701KM232387KC291935
T indicates the ex-type culture. a indicates the newly provided sequences.
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Zeng, Z.-Q.; Zhuang, W.-Y. Three New Species of Fusicolla (Hypocreales) from China. J. Fungi 2023, 9, 572. https://doi.org/10.3390/jof9050572

AMA Style

Zeng Z-Q, Zhuang W-Y. Three New Species of Fusicolla (Hypocreales) from China. Journal of Fungi. 2023; 9(5):572. https://doi.org/10.3390/jof9050572

Chicago/Turabian Style

Zeng, Zhao-Qing, and Wen-Ying Zhuang. 2023. "Three New Species of Fusicolla (Hypocreales) from China" Journal of Fungi 9, no. 5: 572. https://doi.org/10.3390/jof9050572

APA Style

Zeng, Z.-Q., & Zhuang, W.-Y. (2023). Three New Species of Fusicolla (Hypocreales) from China. Journal of Fungi, 9(5), 572. https://doi.org/10.3390/jof9050572

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