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Volume 10
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10.3390/fermentation10110548
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Article

In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa

by
Yong Liu
1,2,
Yiru Zhu
1,3,
Jianping Deng
1,2,
Bing Yan
1,2,
Jian Zhan
3,
Yuansong Wei
4,
Hanbing Nie
1,2,* and
Shuanglin Gui
1,2,*
1
Jiangxi Provincial Key Laboratory of Greenhouse Gas Accounting and Carbon Reduction, Institute of Energy Research, Jiangxi Academy of Sciences, Nanchang 330096, China
2
Jiangxi Carbon Neutalization Research Center, Nanchang 330096, China
3
School of Resources and Environment, Nanchang University, Nanchang 330031, China
4
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
*
Authors to whom correspondence should be addressed.
Fermentation 2024, 10(11), 548; https://doi.org/10.3390/fermentation10110548
Submission received: 23 September 2024 / Revised: 17 October 2024 / Accepted: 23 October 2024 / Published: 25 October 2024
(This article belongs to the Special Issue Progress in Microbial Treatment of Wastewater, Solid Wastes and Waste Gases, 2nd Edition)
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Abstract

Anaerobic ammonium oxidation (anammox), as an efficient and low-carbon method for nitrogen removal from wastewater, faces the challenge of slow enrichment of functional bacteria. In this study, the enrichment of anammox bacteria Candidatus Brocadia was successfully accelerated by co-culturing with the quorum-sensing strain Pseudomonas aeruginosa and anoxic sludge from a pig farm. Experimental results showed that the R2, which had Pseudomonas aeruginosa added, exhibited chemical reaction ratios RS (NO2−-N consumption/NH4+-N consumption) and RP (NO3−-N production/NH4+-N consumption) closer to the theoretical values of the anammox reaction since Phase Ⅱ. Bacterial community analysis indicated that the abundance of Candidatus Brocadia in R2 reached 1.63% in cycle 20, significantly higher than the 0.45% in R1. More quorum-sensing signaling molecules, primarily C6-HSL, were detected in R2. C6-HSL was positively correlated with processes such as the secretion of anammox extracellular polymers (EPS) and the regulation of nitric oxide reductase (Nir), which may explain the reason behind the accelerated increase in the abundance of Candidatus Brocadia through co-culturing. Moreover, the metabolism of the dominant genus Paracoccus within the two groups of reactors also showed positive regulation by C6-HSL, with its abundance trend similar to that of Candidatus Brocadia, jointly completing the nitrogen removal process in the reactors. However, it is still unknown which genera secrete large amounts of C6-HSL after inoculation with Pseudomonas aeruginosa. This research provides a novel and low-cost method for the enrichment of anammox bacteria.
Keywords: anammox; quorum sensing; enrichment; co-cultivation; nitrogen removal anammox; quorum sensing; enrichment; co-cultivation; nitrogen removal

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MDPI and ACS Style

Liu, Y.; Zhu, Y.; Deng, J.; Yan, B.; Zhan, J.; Wei, Y.; Nie, H.; Gui, S. In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa. Fermentation 2024, 10, 548. https://doi.org/10.3390/fermentation10110548

AMA Style

Liu Y, Zhu Y, Deng J, Yan B, Zhan J, Wei Y, Nie H, Gui S. In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa. Fermentation. 2024; 10(11):548. https://doi.org/10.3390/fermentation10110548

Chicago/Turabian Style

Liu, Yong, Yiru Zhu, Jianping Deng, Bing Yan, Jian Zhan, Yuansong Wei, Hanbing Nie, and Shuanglin Gui. 2024. "In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa" Fermentation 10, no. 11: 548. https://doi.org/10.3390/fermentation10110548

APA Style

Liu, Y., Zhu, Y., Deng, J., Yan, B., Zhan, J., Wei, Y., Nie, H., & Gui, S. (2024). In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa. Fermentation, 10(11), 548. https://doi.org/10.3390/fermentation10110548

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