Previous Article in Journal
Comprehensive Review of Strategies for Lactic Acid Bacteria Production and Metabolite Enhancement in Probiotic Cultures: Multifunctional Applications in Functional Foods
Previous Article in Special Issue
Two-Step Bio-Based Production of Heme: In Vivo Cell Cultivation Followed by In Vitro Enzymatic Conversion
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Fermentation
Volume 11
Issue 5
10.3390/fermentation11050242
fermentation-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

High-Level Extracellular Expression of Collagenase ColH in Bacillus subtilis for Adipose-Derived Cells Extraction

by
Ling-Feng Xu
1,
Dai Xue
1,2,
Nuo Chen
1,
Chang Su
1,*,
Jin-Song Gong
1,
Jian-Ying Qian
1,
Zhen-Zhen Wang
1,
Xu-Dong Ma
3,
Nan Xie
3,
Zheng-Hong Xu
4 and
Jin-Song Shi
1,*
1
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China
2
Wuxi Children’s Hospital, Wuxi 214023, China
3
Cytori Therapeutics LLC, Shanghai 201802, China
4
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China
*
Authors to whom correspondence should be addressed.
Fermentation 2025, 11(5), 242; https://doi.org/10.3390/fermentation11050242
Submission received: 25 February 2025 / Revised: 7 April 2025 / Accepted: 8 April 2025 / Published: 24 April 2025
(This article belongs to the Special Issue Applied Microorganisms and Industrial/Food Enzymes, 2nd Edition)
Browse Figures
Versions Notes

Abstract

Collagenase has a wide range of applications in the medicine, cosmetic, and food industries. Inefficient expression of collagenase impedes its industrial production and commercial applications. In this study, a secretory expression system for collagenase ColH from Clostridium histolyticum was constructed in Bacillus subtilis. Signal peptide optimization effectively solved the secretion problem of large collagenase with a molecular weight of about 116 kDa, doubling the extracellular enzyme activity. Then, promoter optimization further improved the enzyme activity to 264 U/mL. By the co-optimization of the nitrogen sources and carbon sources, and employing a fed-batch fermentation strategy, the enzyme activity could reach 669 U/mL, which is, currently, the highest level reported in the industry. The recombinant collagenase ColH was purified through a purification process suitable for industrial production with a specific activity of 565.25 U/mg. Based on the purified collagenase, cells were successfully prepared from adipose tissue, indicating its potential use in cell therapy. This study provides a promising candidate for the industrial production of collagenase and highlights its potential application to extract cells from tissues.
Keywords: collagenase; Bacillus subtilis; extracellular expression; cell extraction collagenase; Bacillus subtilis; extracellular expression; cell extraction
Graphical Abstract

Share and Cite

MDPI and ACS Style

Xu, L.-F.; Xue, D.; Chen, N.; Su, C.; Gong, J.-S.; Qian, J.-Y.; Wang, Z.-Z.; Ma, X.-D.; Xie, N.; Xu, Z.-H.; et al. High-Level Extracellular Expression of Collagenase ColH in Bacillus subtilis for Adipose-Derived Cells Extraction. Fermentation 2025, 11, 242. https://doi.org/10.3390/fermentation11050242

AMA Style

Xu L-F, Xue D, Chen N, Su C, Gong J-S, Qian J-Y, Wang Z-Z, Ma X-D, Xie N, Xu Z-H, et al. High-Level Extracellular Expression of Collagenase ColH in Bacillus subtilis for Adipose-Derived Cells Extraction. Fermentation. 2025; 11(5):242. https://doi.org/10.3390/fermentation11050242

Chicago/Turabian Style

Xu, Ling-Feng, Dai Xue, Nuo Chen, Chang Su, Jin-Song Gong, Jian-Ying Qian, Zhen-Zhen Wang, Xu-Dong Ma, Nan Xie, Zheng-Hong Xu, and et al. 2025. "High-Level Extracellular Expression of Collagenase ColH in Bacillus subtilis for Adipose-Derived Cells Extraction" Fermentation 11, no. 5: 242. https://doi.org/10.3390/fermentation11050242

APA Style

Xu, L.-F., Xue, D., Chen, N., Su, C., Gong, J.-S., Qian, J.-Y., Wang, Z.-Z., Ma, X.-D., Xie, N., Xu, Z.-H., & Shi, J.-S. (2025). High-Level Extracellular Expression of Collagenase ColH in Bacillus subtilis for Adipose-Derived Cells Extraction. Fermentation, 11(5), 242. https://doi.org/10.3390/fermentation11050242

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop