Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY
Abstract
:1. Introduction
2. Materials and Methods
2.1. Algal Strains and Cultivation
2.2. Determination of Biomass Concentration and Biomass Productivity
2.3. Determination of Pigment Contents
2.4. Determination of Chlorophyll Fluorescence
2.5. Determination of Total Carbohydrate Content
2.6. Determination of Total Protein Content
2.7. Determination of Neutral Lipid Content
2.8. Determination of Total Lipid Content and Fatty Acid Composition
2.8.1. Determination of Total Lipid Content
2.8.2. Determination of Fatty Acid Composition
2.9. Statistical Analysis
3. Results
3.1. Analysis of Changes in Growth
3.1.1. The Effect of Glucose Concentration on the Growth of Parachlorella kessleri TY
3.1.2. The Effect of Heterotrophic Culture and Mixotrophic Culture on the Growth of P. kessleri TY
3.2. Analysis of Pigment Content
3.3. Analysis of Chlorophyll Fluorescence Characteristics
3.4. Analysis of Total Carbohydrate Content
3.5. Analysis of Total Protein Content
3.6. Analysis of Neutral Lipid Content
3.7. Analysis of Total Lipid Content and Fatty Acid Composition
3.7.1. Analysis of Total Lipid Content
3.7.2. Analysis of Fatty Acid Composition
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trophic Modes | Biomass Productivity (mg·L−1d−1) |
---|---|
autotrophic | 105.76 b ± 1.22 |
heterotrophic | 77.95 c ± 5.82 |
mixotrophic | 218.43 a ± 10.00 |
Trophic Modes | Lipid Productivity (mg·L−1 d−1) |
---|---|
autotrophic | 35.69 b ± 0.78 |
heterotrophic | 31.00 c ± 0.96 |
mixotrophic | 93.61 a ± 0.37 |
Fatty Acids Composition | Autotrophic (% DW) | Heterotrophic (% DW) | Mixotrophic (% DW) |
---|---|---|---|
C16:0 | 22.00 c ± 0.26 | 23.60 b ± 0.17 | 24.00 a ± 0.07 |
C16:1 | 2.30 c ± 0.05 | 3.31 b ± 0.07 | 3.91 a ± 0.05 |
C16:2 | 6.81 ± 0.06 | - | - |
C18:0 | 7.71 c ± 0.05 | 8.00 b ± 0.08 | 8.32 a ± 0.05 |
C18:1 | 5.31 c ± 0.14 | 15.51 b ± 0.05 | 15.84 a ± 0.03 |
C18:2 | 14.64 c ± 0.11 | 20.50 b ± 0.06 | 21.10 a ± 0.03 |
C18:3 | 28.79 a ± 0.25 | 19.70 b ± 0.07 | 17.95 c ± 0.11 |
C16 + C18 | 87.56 b ± 0.50 | 90.60 a ± 0.08 | 91.12 a ± 0.06 |
others | 12.44 a ± 0.50 | 9.40 b ± 0.80 | 8.88 b ± 0.06 |
SFA | 33.93 c ± 0.06 | 34.88 b ± 0.24 | 35.47 a ± 0.15 |
MUFA | 8.69 c ± 0.06 | 20.77 b ± 0.14 | 21.67 a ± 0.08 |
PUFA | 57.38 a ± 0.11 | 44.37 b ± 0.06 | 42.85 c ± 0.08 |
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Gao, Y.; Li, Y.; Yang, Y.; Feng, J.; Ji, L.; Xie, S. Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY. Fermentation 2023, 9, 891. https://doi.org/10.3390/fermentation9100891
Gao Y, Li Y, Yang Y, Feng J, Ji L, Xie S. Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY. Fermentation. 2023; 9(10):891. https://doi.org/10.3390/fermentation9100891
Chicago/Turabian StyleGao, Yifan, Yuan Li, Yan Yang, Jia Feng, Li Ji, and Shulian Xie. 2023. "Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY" Fermentation 9, no. 10: 891. https://doi.org/10.3390/fermentation9100891