Abstract
Insight into bacterial cellular composition and functions can be obtained by the application of metabolomics. Metabolomics enables comprehensive characterization of a set of low-molecular-weight compounds which are starting, intermediate, or end products of metabolic transformations in living organisms. Metabolites are directly related to biochemical, physiological, and pathophysiological processes and they reflect the activity of genes at a particular time in a given environment. They provide information about how the environment affects organisms, including the action of potential drugs. Metabolomic intracellular fingerprinting and extracellular footprinting can provide complementary and classifying information about the mechanisms of action of antibacterial compounds. Both sets of metabolites are affected in a distinct manner when bacteria are under the influence of compounds with intracellular or extracellular targets. Liquid chromatography–mass spectrometry supported by bioinformatics was successfully applied to monitor compounds in mycobacterial cultures. Changes in tuberculosinyladenosine levels or in metabolites related to energy pools (e.g., nicotinic acid ribonucleotide, acetyl phosphate, adenosine 5′-monophosphate, and nicotinamide adenine dinucleotide) were detected after bacterial exposure to natural products.
Funding
This work was funded by the Polish National Science Centre within the project 2022/45/B/NZ7/00412.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Data relevant to the presented talk are available from the author.
Conflicts of Interest
The author declares no conflict of interest.
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