Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression
Abstract
:1. Introduction
2. Patients and Methods
2.1. Study Design and Tissue Samples
2.2. Mucociliary Transport Velocity (MCTV) Measurement
2.3. Scanning Electron Microscopy (SEM)
2.4. Total RNA Extraction and Quantitative RT-PCR Analysis
2.5. Immunohistochemistry and Laser Scanning Confocal Microscopy (LSCM) Imaging
2.6. Data Analysis
3. Results
3.1. Background and Characteristics of Subjects in the Study
3.2. Mucociliary Transport Velocity Assessment
3.3. SEM Observation
3.4. Target Gene Expressions in Sinonasal Mucosa and Nasal Polyps
3.4.1. Comparison of mRNA Expressions of PCP Proteins between the Controls and CRS Patients
3.4.2. Correlation with Inflammatory Cytokines
3.5. Immunohistochemical Observation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Controls | CRSsNP | CRSwNP | |
---|---|---|---|
n (male/female) | 30 (17/13) | 25 (13/12) | 28 (15/13) |
Age, mean ± SD | 44.0 ± 17.7 | 60.4 ± 15.0 ††† | 54.9 ± 10.2 †† |
BMI, kg/mm2, mean ± SD | 22.9 ± 4.0 | 22.4 ± 2.8 | 22.6 ± 2.8 |
Allergic rhinitis, % | 19 (63.3%) | 12 (48%) | 19 (67.8%) |
Bronchial asthma, % | 2 (6.7%) | 0 (0%) | 9 (32.14%) **,† |
CT score, mean ± SD | NA | 3.75 ± 3.5 | 12.75 ± 4.98 **** |
Blood eosinophils, %; median, range | 4.4 (0.3–4.4) | 1.9 (0.3–14.4) | 5.55 (0.3–13.9) *** |
Tissue eosinophils, cells/HPF; median, range | 4.8 (0.0–103.3) | 2.6 (0.0–45) | 74.2 (0.0–333.3) *** |
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Hirokane, S.; Kawasumi, T.; Takeno, S.; Okamoto, Y.; Miyamoto, S.; Fujita, R.; Ishikawa, C.; Oda, T.; Horibe, Y.; Ishino, T.; et al. Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression. Immuno 2024, 4, 247-265. https://doi.org/10.3390/immuno4030016
Hirokane S, Kawasumi T, Takeno S, Okamoto Y, Miyamoto S, Fujita R, Ishikawa C, Oda T, Horibe Y, Ishino T, et al. Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression. Immuno. 2024; 4(3):247-265. https://doi.org/10.3390/immuno4030016
Chicago/Turabian StyleHirokane, Sakura, Tomohiro Kawasumi, Sachio Takeno, Yukako Okamoto, Seita Miyamoto, Rikuto Fujita, Chie Ishikawa, Takashi Oda, Yuichiro Horibe, Takashi Ishino, and et al. 2024. "Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression" Immuno 4, no. 3: 247-265. https://doi.org/10.3390/immuno4030016