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Article

Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus

by
César Augusto Sodré da Silva
1,
Karen Vanessa Marimón Sibaja
1,
Sabrina de Ramos Cizilio
2,
José Roberto Miranda Júnior
1,
Rejane de Castro Santana
2,
Marcio Arêdes Martins
3,
Maurício de Oliveira Leite
3,
Eduardo Basílio de Oliveira
1 and
Jane Sélia dos Reis Coimbra
1,*
1
Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Campus Universitário S/N, Centro, Viçosa 36570-900, Brazil
2
Departamento de Química, Universidade Federal de Viçosa, Campus Universitário S/N, Centro, Viçosa 36570-900, Brazil
3
Departamento de Engenharia Agrícola, Universidade Federal de Viçosa, Campus Universitário S/N, Centro, Viçosa 36570-900, Brazil
*
Author to whom correspondence should be addressed.
Phycology 2024, 4(1), 87-103; https://doi.org/10.3390/phycology4010005
Submission received: 30 September 2023 / Revised: 28 December 2023 / Accepted: 23 January 2024 / Published: 30 January 2024
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Abstract

The high protein content of several microalgal species makes them attractive and unconventional candidates for use in the food and pharmaceutical industries. Due to the robust cell walls of microalgae, cell rupture is necessary to improve the extraction of intracellular proteins. Thus, choosing a suitable cell-breaking treatment before protein extraction is a vital downstream processing step. Additionally, it is necessary to use an effective technique for monitoring and measuring the impact of rupture treatments on microalgal cell walls. In our study, Tetradesmus obliquus cells were disrupted using three different mechanical rupture methods: high-pressure homogenization (HPH), ultrasound (US), and ball milling (BM). The ruptured biomass cells were counted, and soluble proteins were extracted and quantified. The cell-counting technique did not detect any differences between intact and damaged cells after BM treatment because the dye (erythrosine B) did not permeate the microalgal biomass accurately. The US treatment promoted the highest yield of total protein extraction (19.95%), while the highest yields in the HPH and BM treatments were 15.68% and 14.11%, respectively. Since the cell breakage method affects protein extraction from microalgal biomass, protein release can be used as a central indicator of the degree of cell disruption.
Keywords: ball milling; high-pressure homogenization; ultrasonication; cell counting ball milling; high-pressure homogenization; ultrasonication; cell counting

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MDPI and ACS Style

da Silva, C.A.S.; Sibaja, K.V.M.; de Ramos Cizilio, S.; Miranda Júnior, J.R.; de Castro Santana, R.; Martins, M.A.; de Oliveira Leite, M.; de Oliveira, E.B.; Coimbra, J.S.d.R. Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus. Phycology 2024, 4, 87-103. https://doi.org/10.3390/phycology4010005

AMA Style

da Silva CAS, Sibaja KVM, de Ramos Cizilio S, Miranda Júnior JR, de Castro Santana R, Martins MA, de Oliveira Leite M, de Oliveira EB, Coimbra JSdR. Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus. Phycology. 2024; 4(1):87-103. https://doi.org/10.3390/phycology4010005

Chicago/Turabian Style

da Silva, César Augusto Sodré, Karen Vanessa Marimón Sibaja, Sabrina de Ramos Cizilio, José Roberto Miranda Júnior, Rejane de Castro Santana, Marcio Arêdes Martins, Maurício de Oliveira Leite, Eduardo Basílio de Oliveira, and Jane Sélia dos Reis Coimbra. 2024. "Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus" Phycology 4, no. 1: 87-103. https://doi.org/10.3390/phycology4010005

APA Style

da Silva, C. A. S., Sibaja, K. V. M., de Ramos Cizilio, S., Miranda Júnior, J. R., de Castro Santana, R., Martins, M. A., de Oliveira Leite, M., de Oliveira, E. B., & Coimbra, J. S. d. R. (2024). Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus. Phycology, 4(1), 87-103. https://doi.org/10.3390/phycology4010005

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